Time Correlated Single Photon Counting
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Time Correlated Single Photon Counting
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Author : Desmond O'Connor
language : en
Publisher: Academic Press
Release Date : 2012-12-02
Time Correlated Single Photon Counting written by Desmond O'Connor and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.
Advanced Time Correlated Single Photon Counting Techniques
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Author : Wolfgang Becker
language : en
Publisher: Springer Science & Business Media
Release Date : 2005-12-19
Advanced Time Correlated Single Photon Counting Techniques written by Wolfgang Becker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-12-19 with Science categories.
In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.
Advanced Photon Counting
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Author : Peter Kapusta
language : en
Publisher: Springer
Release Date : 2015-04-23
Advanced Photon Counting written by Peter Kapusta and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-23 with Science categories.
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
Advanced Time Correlated Single Photon Counting Applications
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Author : Wolfgang Becker
language : en
Publisher: Springer
Release Date : 2015-04-13
Advanced Time Correlated Single Photon Counting Applications written by Wolfgang Becker and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-13 with Science categories.
This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.
Photon Counting Image Sensors
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Author : Eric R. Fossum
language : en
Publisher: MDPI
Release Date : 2018-07-06
Photon Counting Image Sensors written by Eric R. Fossum and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-07-06 with categories.
This book is a printed edition of the Special Issue "Photon-Counting Image Sensors" that was published in Sensors
Photon Counting
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Author : Nikolay Britun
language : en
Publisher: BoD – Books on Demand
Release Date : 2018-03-21
Photon Counting written by Nikolay Britun and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-21 with Science categories.
Photon counting is a unified name for the techniques using single-photon detection for accumulative measurements of the light flux, normally occurring under extremely low-light conditions. Nowadays, this approach can be applied to the wide variety of the radiation wavelengths, starting from X-ray and deep ultraviolet transitions and ending with far-infrared part of the spectrum. As a special tribute to the photon counting, the studies of cosmic microwave background radiation in astronomy, the experiments with muon detection, and the large-scale fundamental experiments on the nature of matter should be noted. The book provides readers with an overview on the fundamentals and state-of-the-art applications of photon counting technique in the applied science and everyday life.
Advanced Photon Counting
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Author : Peter Kapusta
language : en
Publisher:
Release Date : 2015
Advanced Photon Counting written by Peter Kapusta and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.
A Novel Wide Field Time Correlated Single Photon Counting Technique For Luminescence Lifetime Imaging
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Author : Nicolas Sergent
language : en
Publisher:
Release Date : 2009
A Novel Wide Field Time Correlated Single Photon Counting Technique For Luminescence Lifetime Imaging written by Nicolas Sergent and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009 with Fluorescence categories.
This Thesis presents the development and characterisation of a novel wide field, time-correlated single photon technique for luminescence lifetime imaging, and its possible use for life sciences imaging. -- The primary device which was developed is based on a 3-stage image intensifier coupled with a high speed CMOS camera, mounted on a conventional microscope. The post-acquisition image processing software developed for this purpose, in¬volving photon detection and centroiding algorithms, is described and discussed. Lifetime images of artificial ruby and europium compounds are reported, as well as other applications of single photon counting imaging for fluorescence microscopy. Another wide-field TCSPC technique, based on an Electron-Bombarded CCD de¬tector, is presented, along with the characterisation of the device, and its uses as a single photon counting detector for wide-field microscopy. -- This work also presents results of Fluorescence Lifetime Imaging Microscopy and Time-Resolved Anisotropy measurements of biological samples on a commercial laser scanning microscope system. -- Finally, this work presents suggestions of improvement and further developments on these wide-field TCSPC detectors.
The Bh Tcspc Handbook
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Author : Wolfgang Becker
language : en
Publisher:
Release Date : 2012
The Bh Tcspc Handbook written by Wolfgang Becker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Single Molecule Spectroscopy
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Author : R. Rigler
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Single Molecule Spectroscopy written by R. Rigler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.