Advanced Photon Counting

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Advanced Photon Counting

Advanced Photon Counting Author by : Peter Kapusta
Languange Used : en
Release Date : 2015-04-23
Publisher by : Springer
ISBN : 9783319156361
File Size : 40,8 Mb
Total Download : 373

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonan
Category: Science

Advanced Time Correlated Single Photon Counting Techniques

Advanced Time Correlated Single Photon Counting Techniques Author by : Wolfgang Becker
Languange Used : en
Release Date : 2005-12-20
Publisher by : Springer Science & Business Media
ISBN : 3540288821
File Size : 55,8 Mb
Total Download : 750

In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimension
Category: Technology & Engineering

Time Correlated Single Photon Counting

Time Correlated Single Photon Counting Author by : Desmond O'Connor
Languange Used : en
Release Date : 2012-12-02
Publisher by : Academic Press
ISBN : 9780323141444
File Size : 55,6 Mb
Total Download : 710

Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time depen
Category: Science
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