Adipose Tissue Protocols
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Adipose Tissue Protocols
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Author : Gérard Ailhaud
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Adipose Tissue Protocols written by Gérard Ailhaud and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Adipose tissue is recognized to be exquisitely sensitive to hormone action, and is also now recognized as a secretory and endocrine organ required for reproduction and good health. Adipocytes are “smart” cells able within the tissue to communicate with surrounding cells, but also with various organs, particularly via leptin acting on the central nervous system. Brown adipose tissue (BAT) and white adipose tissue (WAT) are known to be distinct tissues, whereas the heterogeneity of WAT depots is well est- lished. Unfortunately, excess WAT leads to obesity, which is the most common health problem in industrialized countries. Therefore, from both a scientific and a technical point of view, the time has come to create a survey of adipose tissues and their neglected adipocytes. In Adipose Tissue Protocols, I have attempted to gather together chapters from all areas of adipose tissue research—from in vivo to in vitro studies—and to provide methods covering a wide variety of techniques, including the choice of adipose tissue depot and of morphological techniques for the study of BAT and WAT; the isolation, subcellular fractionation, and transfection of adipocytes where the low density of these cells must be taken into account; assays of nutrient and ion fluxes and the metabolic aspects of nutrient uptake; assays of lipid-related enzymes; biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT of various species, including human tissue; measurements of adipose secretory products; and assessment of WAT metabolism in vivo.
Human Cell Culture Protocols
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Author : Joanna Picot
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Human Cell Culture Protocols written by Joanna Picot and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Adipose Derived Stem Cells
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Author : Jeffrey M. Gimble
language : en
Publisher: Humana Press
Release Date : 2010-11-19
Adipose Derived Stem Cells written by Jeffrey M. Gimble and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-19 with Science categories.
During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular BiologyTM series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.
Hormones Metabolism And The Benefits Of Exercise
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Author : Bruce Spiegelman
language : en
Publisher: Springer
Release Date : 2018-03-07
Hormones Metabolism And The Benefits Of Exercise written by Bruce Spiegelman and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-07 with Science categories.
The world is faced with an epidemic of metabolic diseases such as obesity and type 2 diabetes. This is due to changes in dietary habits and the decrease in physical activity. Exercise is usually part of the prescription, the first line of defense, to prevent or treat metabolic disorders. However, we are still learning how and why exercise provides metabolic benefits in human health. This open access volume focuses on the cellular and molecular pathways that link exercise, muscle biology, hormones and metabolism. This will include novel “myokines” that might act as new therapeutic agents in the future.
Handbook Of Obesity
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Author : W. P. T. James
language : en
Publisher: CRC Press
Release Date : 2003-12
Handbook Of Obesity written by W. P. T. James and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-12 with Medical categories.
Offering perspectives on the history, prevalence and genetics of obesity, this book examines the origins and etiology of obesity. It considers the relationship between behavioural neuroscience and obesity.
Adipose Tissue Biology
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Author : Michael E. Symonds
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-11-20
Adipose Tissue Biology written by Michael E. Symonds and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-20 with Medical categories.
This book is designed to provide a comprehensive insight into current perspectives and challenges in adipose tissue biology. In Adipose Tissue Biology, scientists and clinicians discuss adipocyte precursors, differentiation and growth, brown and white adipose tissue, gender, inflammation, dietary and genetic determinants of fat mass, together with evolutionary and developmental aspects of adiposity.
Physiology And Physiopathology Of Adipose Tissue
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Author : Jean-Philippe Bastard
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-11-28
Physiology And Physiopathology Of Adipose Tissue written by Jean-Philippe Bastard and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-11-28 with Medical categories.
The scientific advances in the physiology and pathophysiology of adipose tissue over the last two decades have been considerable. Today, the cellular and molecular mechanisms of adipogenesis are well known. In addition, adipose tissue is now recognized as a real endocrine organ that produces hormones such as the leptin acting to regulate food intake and energy balance in the central nervous system, a finding that has completely revolutionized the paradigm of energy homeostasis. Other adipokines have now been described and these molecules are taking on increasing importance in physiology and pathophysiology. Moreover, numerous works have shown that in obesity, but also in cases of lipodystophy, adipose tissue was the site of a local low-grade inflammation that involves immune cells such as macrophages and certain populations of lymphocytes. This new information is an important step in the pathophysiology of both obesity and related metabolic and cardiovascular complications. Finally, it is a unique and original work focusing on adipose tissue, covering biology and pathology by investigating aspects of molecular and cellular biology, general, metabolic, genetic and genomic biochemistry.
Human Cell Culture Protocols
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Author : Ragai R. Mitry
language : en
Publisher: Humana Press
Release Date : 2016-08-23
Human Cell Culture Protocols written by Ragai R. Mitry and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Science categories.
Human cell culture is not a new topic, but the development of new molecular techniques and reagents which can be used to investigate cell function and the responsible intracellular mechanisms make it a continuing requirement. This third edition of Human Cell Culture Protocols expands upon the previous editions with current, detailed protocols for the isolation and culture of a range of primary cells from human tissues. With new chapters on pancreatic cells needed for basic studies on the pathogenesis of diabetes and for their application for islet transplantation, the book also delves into protocols for hepatocytes, skin cells, lung cells, parathyroid cells, gastric cells, renal cells, adipocytes, ovarian cells, bone cells, vascular smooth muscle cells, vascular endothelial cells, regulatory T cells, blood mononuclear cells, as well as new techniques being applied to human cell culture, particularly the use of biocompatible scaffolds to grow cells, the in vitro use of laser microdissection to isolate cells from culture, and automated cell culture. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Human Cell Culture Protocols, Third Edition makes it possible for a worker with basic cell culture training, whether in the fields of cell biology, gene therapy, and cell transplantation, to prepare cell cultures of the specific cell type necessary to forward their vital research.
Best Practice Protocols For Physique Assessment In Sport
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Author : Patria A. Hume
language : en
Publisher: Springer
Release Date : 2017-12-21
Best Practice Protocols For Physique Assessment In Sport written by Patria A. Hume and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-21 with Medical categories.
This book outlines best practice protocols for body composition analysis of high-performance athletes and provides guidance on the use of new technologies for anthropometry. While surface anthropometry has traditionally been used to assess body composition through the internationally recognised methodology of the International Society for the Advancement of Kinanthropometry (ISAK), the recent commercialisation of devices, such as bioelectrical impedance technologies, dual-energy X-ray absorptiometry (DXA), ultrasound, 3D photometry and air-displacement plethysmography (Bod Pod), has led to the non-standardised adoption of new measurement techniques. In this textbook, leading researchers detail standardisation procedures for each technology: in terms of athlete preparation, test protocols, test reporting, equipment calibration and data interpretation. Each chapter covers a different tool: how it works, what it is used to measure, and what the issues are surrounding its validity, practicality and reliability. This book provides an essential reference for device technicians and sport practitioners, ensuring that high-performance athletes are afforded accurate and comparable body composition information to guide their training routines.
Mdct Protocols
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Author : Andrea Laghi
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-04-23
Mdct Protocols written by Andrea Laghi and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-23 with Medical categories.
Multidetector-row computed tomography (MDCT) has become a fundamental imaging technique for the study of many anatomical districts in different clinical situations, as it provides a fast, reliable, and accurate simultaneous evaluation of different organs, including parenchyma, hollow viscera, vessels, and bony structures. It has also equipped the radiologist with the ability to explore areas that in the last decade were largely ignored by CT, especially the coronary arteries and the colon, and to limit invasive diagnostic tests (e.g., catheter angiography) to those cases in which interventional procedures are required. The examination quality and the consequent diagnostic accuracy of MDCT are the results of an optimized study technique, which nonetheless needs to be adapted to the particular clinical situation of the patient, while bearing in mind the radiation exposure. Another fundamental parameter in the optimization of MDCT is the protocol for the intravenous injection of iodinated contrast material; in these studies, multiple variables, some modifiable by the operator and others patient-dependent, must be considered based on the aims of maximizing arterial and venous enhancements whilst minimizing the dose of iodine injected, saving money, and increasing patient safety. Through the presentation of cases addressing different anatomical regions and various clinical indications, including emergency and neuro-imaging, this volume seeks to provide the general radiologist and trainee specialist with a guide to the main study protocols to be implemented in order to optimize examination quality and, consequently, facilitate the diagnostic process.