Advanced Cell And Molecular Techniques

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Advanced Methods In Molecular Biology And Biotechnology
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Author : Khalid Z. Masoodi
language : en
Publisher: Academic Press
Release Date : 2020-10-28
Advanced Methods In Molecular Biology And Biotechnology written by Khalid Z. Masoodi and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-28 with Science categories.
Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. - Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology - Features clear, step-by-step instruction for applying the techniques covered - Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2004
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.
Advanced Cell And Molecular Techniques
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Author : Karuppusamy Arunachalam
language : en
Publisher: Springer Nature
Release Date : 2025-04-23
Advanced Cell And Molecular Techniques written by Karuppusamy Arunachalam and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-04-23 with Science categories.
This detailed volume explores laboratory protocols and methodologies for studying cell cultures, genetic analysis, and various biochemical assays. The chapters within this book cover a wide range of techniques, from basic cell culture methods to more specialized assays used in the study of genotoxicity, apoptosis, and gene expression, not only relevant to academic researchers but also to professionals in the pharmaceutical, biotechnological, and clinical fields. Written for the Springer Protocols Handbooks series, each protocol has been carefully compiled to provide step-by-step instructions, ensuring clarity and reproducibility. Authoritative and practical, Advanced Cell and Molecular Techniques: Protocols for In Vitro and In Vivo Studies serves as both a hands-on guide and a source of inspiration for future research as the fields of molecular biology and toxicology continue to evolve.
Molecular Biology Techniques
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Author : Heather B. Miller
language : en
Publisher: Academic Press
Release Date : 2011-10-18
Molecular Biology Techniques written by Heather B. Miller and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-10-18 with Science categories.
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. - Cover basic concepts and techniques used in molecular biology research labs - Student-tested labs proven successful in a real classroom laboratories - Exercises simulate a cloning project that would be performed in a real research lab - "Project" approach to experiments gives students an overview of the entire process - Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2010-03-04
Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-04 with Medical categories.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Advances In Cell And Molecular Diagnostics
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Author : P.B. Raghavendra
language : en
Publisher: Academic Press
Release Date : 2018-01-02
Advances In Cell And Molecular Diagnostics written by P.B. Raghavendra and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-02 with Science categories.
Advances in Cell and Molecular Diagnostics brings the scientific advances in the translation and validation of cellular and molecular discoveries in medicine into the clinical diagnostic setting. It enumerates the description and application of technological advances in the field of cellular and molecular diagnostic medicine, providing an overview of specialized fields, such as biomarker, genetic marker, screening, DNA-profiling, NGS, cytogenetics, transcriptome, cancer biomarkers, prostate specific antigen, and biomarker toxicologies. In addition, it presents novel discoveries and clinical pathologic correlations, including studies in oncology, infectious diseases, inherited diseases, predisposition to disease, and the description or polymorphisms linked to disease states. This book is a valuable resource for oncologists, practitioners and several members of the biomedical field who are interested in understanding how to apply cutting-edge technologies into diagnostics and healthcare. - Encompasses the current scientific advances in the translation and validation of cellular and molecular discoveries into the clinical diagnostic setting - Explains the application of cellular and molecular diagnostics methodologies in clinical trials - Focuses on translating preclinical tests to the bedside in order to help readers apply the most recent technologies to healthcare
Advanced Lab Practices In Biochemistry Molecular Biology
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Author : Swati Agarwal
language : en
Publisher: I K International Pvt Ltd
Release Date : 2018-08-10
Advanced Lab Practices In Biochemistry Molecular Biology written by Swati Agarwal and has been published by I K International Pvt Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-10 with Medical categories.
This book provides detailed information on various instruments, techniques and experiment protocols of biochemistry and molecular biology. It deals with basic as well as advanced information and in-depth methodology in simple language to help students and professionals to perform experiments with ease. This book not only clears the practical concepts of Biochemistry and Molecular Biology at undergraduate and post-graduation levels, but also helps to pass the Ph.D. course work exam conducted by various universities. This book will develop research aptitude to clear the NET examination. This manual gives a comprehensive idea about the various instruments, their working, troubleshooting and their applications. It provides a wide spectrum of 14 chapters covering basic as well as advanced techniques and instrumentation, viz., Gas Chromatography (GC), Mass Spectrometry (MS), Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) with detailed protocols. Most of the experiments can be easily performed in the laboratory having basic facilities. Historical background, experiment nature, its principle, step-by-step procedure with diagrammatic representation and important precautions are given in the beginning of each experiment.
Advanced Cell And Molecular Biology
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Author : Bolakale Aremu
language : en
Publisher: AB Publisher LLC
Release Date : 2025-03-30
Advanced Cell And Molecular Biology written by Bolakale Aremu and has been published by AB Publisher LLC this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-30 with Science categories.
Unlock the Secrets of the Cell—Beyond the Basics. Are you ready to move beyond foundational biology and dive into the cutting edge of modern science? Advanced Cell and Molecular Biology is your gateway to the next level of understanding. Crafted for upper-level students, researchers, and professionals, this advanced guide explores the intricate molecular mechanisms that govern life, the transformative technologies revolutionizing biological research, and the discoveries shaping the future of medicine, genetics, and biotechnology. From CRISPR gene editing and chromatin remodeling to single-cell analysis, synthetic biology, and cancer cell signaling, each chapter provides a research-informed deep dive into the molecular heartbeat of cells. What sets this book apart is its modular and concise structure, carefully designed to maximize clarity and learning efficiency without sacrificing depth. The bullet-point format and focused subtopics allow readers to quickly grasp complex ideas, making the book ideal for fast reference, exam prep, or on-the-go research support. Inside you'll find: > Clear, concise explanations of complex biological processes > High-quality illustrations and diagrams for enhanced understanding > Up-to-date research insights and real-world scientific applications > A modular chapter layout for targeted, flexible learning > Compact sections that support both deep study and quick review Whether you're preparing for graduate school, conducting cutting-edge research, or working in the biotech or biomedical industries, this book will sharpen your expertise and expand your scientific perspective. Explore the frontier of life science. Master the complexity of the cell—with precision, clarity, and insight.
Molecular Neuroscience
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Author : Rusty Lansford
language : en
Publisher:
Release Date : 2014
Molecular Neuroscience written by Rusty Lansford and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Genes categories.
A wide variety of powerful molecular techniques have been applied to biology in recent decades, ranging from recombinant DNA technologies to state-of-the-art imaging methods. But the plethora of techniques available combined with the complexities of neurobiological systems can make it difficult for neuroscientists to select and carry out an experimental procedure to effectively address the question at hand. This laboratory manual serves as a comprehensive practical guide to molecular and cellular methods for neuroscientists. It consists of five major sections: Working with Cells, Working with DNA, Working with RNA, Gene Transfer, and Imaging. Each includes step-by-step protocols and discussions of basic and cutting-edge procedures for working in that area. Fundamental techniques include maintaining a sterile working environment, purifying and culturing neural cells, isolating and manipulating DNA and RNA, and understanding and using a microscope. Advanced topics include single-neuron isolation and analysis, in vivo gene delivery and imaging, optogenetics, RNA interference, transgenic technologies, high-throughput analysis of gene expression (e.g., RNA-Seq), and constructing and imaging fluorescent proteins. The manual includes protocols developed in the Advanced Techniques in Molecular Neuroscience course offered annually at Cold Spring Harbor Laboratory, as well as protocols drawn from its best-selling lab manuals. It is an essential resource for all neuroscientists, from graduate students upward, who seek to use molecular techniques to probe the complexities of the nervous system.
Introduction To Cell And Tissue Culture
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Author : Jennie P. Mather
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-08-20
Introduction To Cell And Tissue Culture written by Jennie P. Mather and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-08-20 with Science categories.
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.