Antigen Processing And Presentation Protocols
DOWNLOAD
Download Antigen Processing And Presentation Protocols PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Antigen Processing And Presentation Protocols book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Antigen Processing And Presentation Protocols
DOWNLOAD
Author : Joyce C. Solheim
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-10-26
Antigen Processing And Presentation Protocols written by Joyce C. Solheim and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-10-26 with Medical categories.
Antigen processing and presentation, as a field, explores a broad range of protein interactions and functions, both intracellular (in the cytoplasm and in the endoplasmic reticulum) and at the cell surface (between T cells and MHC molecules). To investigate such a diverse array, it is necessary that biochemical, cell biology, and immunological techniques all be employed. The purpose of Antigen Processing and Presentation Protocols is therefore to detail the most up-to-date techniques being used in this burgeoning field. Such techniques include those used to question how MHC-binding peptides are generated, to test how peptides are delivered to MHC molecules, to analyze MHC peptide-binding patterns, and to assay the T-cell response to MHC/peptide. Antigen Processing and Presentation Protocols should aid both those new and those experienced in this area of research in extending the questions that can be asked and answered by the application of these current methods. For editorial assistance, I would like to thank Angela Beninga and Rachael Turnquist.
Protein Structure Stability And Folding
DOWNLOAD
Author : Kenneth P. Murphy
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Protein Structure Stability And Folding written by Kenneth P. Murphy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.
Macrophages
DOWNLOAD
Author : Donna M. Paulnock
language : en
Publisher: OUP Oxford
Release Date : 2000-09-14
Macrophages written by Donna M. Paulnock and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-09-14 with Science categories.
Macrophages are an important part of the immune response and are characterized by their ability to phagocytose foreign matter. However the difficulties involved in macrophage isolation mean they are some of the body's least explored cells. Macrophage Methodology describes how to isolate moderate to high yields of viable cells from a variety of specific tissue sites under both normal and pathological conditions and then goes on to give protocols for macrophage purification. The third chapter covers techniques used to identify and measure endocytic and phagocytic capabilities using immunochemistry and fluorescent analysis. Chapter four identifies the key issues relating to the study of macrophages as antigen presenting cells and has protocols for the major assays used to measure antigen processing and presentation. Also covered are the theoretical and practical issues related to the processing and presentation of intracellular pathogens for which macrophages are the major host cell. The methods described for measuring macrophage secretory products concentrate on bioassays for molecules where no ELISA is available. The next two chapters cover measuring macrophage activity in vitro and in vivo. Finally methods are described for the analysis of gene expression in macrophages. A variety of broad techniques have been brought together in one affordable volume to make Macrophage Methodology an essential buy for anyone studying macrophages.
Capillary Electrophoresis Of Nucleic Acids
DOWNLOAD
Author : Keith R. Mitchelson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Capillary Electrophoresis Of Nucleic Acids written by Keith R. Mitchelson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
The Elisa Guidebook
DOWNLOAD
Author : John R. Crowther
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
The Elisa Guidebook written by John R. Crowther and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research.
Biostatistical Methods
DOWNLOAD
Author : Stephen W. Looney
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Biostatistical Methods written by Stephen W. Looney and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Leading biostatisticians and biomedical researchers describe many of the key techniques used to solve commonly occurring data analytic problems in molecular biology, and demonstrate how these methods can be used in the development of new markers for exposure to a risk factor or for disease outcomes. Major areas of application include microarray analysis, proteomic studies, image quantitation, genetic susceptibility and association, evaluation of new biomarkers, and power analysis and sample size.
Vaccinology
DOWNLOAD
Author : W. John W. Morrow
language : en
Publisher: John Wiley & Sons
Release Date : 2012-09-04
Vaccinology written by W. John W. Morrow and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-04 with Medical categories.
Covering all aspects of vaccine research and development in one volume, this authoritative resource takes a comprehensive and systematic approach to the science of vaccinology focusing not only on basic science, but also on the many stages required to commercialize and navigate the regulatory requirements for human application, both in the United States and Europe. Reviews in detail the process of designing a vaccine, from the initial stages of antigen discovery to human application Includes evaluation of vaccine efficacy and safety Details clinical trial design, including regulatory requirements Discusses the emerging field of active cellular immunotherapy Vaccinology: Principles and Practice provides an invaluable resource for clinicians, scientific and medical researchers, lecturers and postdoctoral fellows working in the field of vaccines.
Antigen Presenting Cells
DOWNLOAD
Author : Lawrence B. Schook
language : en
Publisher:
Release Date : 1988
Antigen Presenting Cells written by Lawrence B. Schook and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Medical categories.
Biochemicals And Reagents
DOWNLOAD
Author :
language : en
Publisher:
Release Date :
Biochemicals And Reagents written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on with categories.
Microbes As Tools For Cell Biology
DOWNLOAD
Author :
language : en
Publisher: Academic Press
Release Date : 1995-02-08
Microbes As Tools For Cell Biology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995-02-08 with Medical categories.
Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents.Key Features* Introduction by renowned microbiologist Dr. Stanley Falkow* Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes* Guides researchers in the study of intracellular pathogenesis* Describes microbial adherence and phagocytosis assays* Focuses on protein trafficking in infected cells* Well-illustrated with color plates, halftones, and diagrams