Applications Of Pcr In Mycology

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Applications Of Pcr In Mycology

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Author : P. D. Bridge
language : en
Publisher: CABI
Release Date : 1998-01-01

Applications Of Pcr In Mycology written by P. D. Bridge and has been published by CABI this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-01-01 with Science categories.

Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.

Applications Of Pcr In Mycology

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Author : P. D. Bridge
language : en
Publisher:
Release Date : 1998

Applications Of Pcr In Mycology written by P. D. Bridge and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with categories.

Polymerase chain reaction in mycology: an overview; Gene cloning using PCR; DDRT-PCR for screening of fungal gene expression; Interpretation of PCR methods for species definition; PCR applications in studies of lichen-forming fungi; Applications of PCR for studying the biodiversity of mycorrhizal fungi; PCR applications in fungal phylogeny; PCR applications to the taxonomy of entomopathogenic fungi; Application of PCR in fungal biotechnology; Application of PCR in studying lignocellulose degradation by basidiomycetes; PCR methdos for the detection o mycotoxin-producing fungi; PCR diagnostics in medical mycology; Applications of PCR in fungal-plant interactions; PCR as a tool for the investigation of seed-borne disease; Future directions for PCR in mycology.

Molecular Markers In Mycology

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Author : Bhim Pratap Singh
language : en
Publisher: Springer
Release Date : 2017-01-21

Molecular Markers In Mycology written by Bhim Pratap Singh and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-21 with Science categories.

The Kingdom fungi encompass a massive diversity of taxa with wide-ranging ecologies, life cycles, and morphologies ranging from unicellular aquatic chytrids to large mushrooms. Before molecular methods came in existence, taxonomists considered this Kingdom to be a member of the plant kingdom due to certain life styles like immobility and growth habitats. Molecular markers (also known as DNA markers), facilitated a better alternative method over traditional morphological methods, employed for the identification, characterization, and to understand the evolution of fungi. The morphological methods used for identification are mainly dependent on spore color or microscopic features whereas molecular markers are based on DNA polymorphism in the genomic organization. Phylogenetic studies reported in last decade, based on molecular markers, have reshaped the classification system of Kingdom fungi, which divided into one subkingdom, seven phyla, and ten subphyla. Recent advances in molecular mycology have opened the way for researchers to identify and characterize novel fungal species from unique environments. Mycology is concerned with the systematic study of fungi, including their genetic and biochemical properties, their use to humans as a source of medicine and food, as well as their dangers, such as poisoning and infections. In the 21st century with the development of DNA sequencing technologies and phylogenetic analysis based on molecular markers, new insights into fungal taxonomy were provided. This book contains a thorough discussion of molecular characterization and detection of different groups of fungi by using PCR-based markers and provides a comprehensive view of the applications and uses of different molecular markers in molecular mycology. It also addresses the recent molecular markers employed to solve the problems of identification and discusses current approaches used in molecular characterization and detection of fungi.

Pcr Protocols

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Author : Michael A. Innis
language : en
Publisher: Academic Press
Release Date : 2012-12-02

Pcr Protocols written by Michael A. Innis and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications

Monitoring Antagonistic Fungi Deliberately Released Into The Environment

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Author : Dan Funck Jensen
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Monitoring Antagonistic Fungi Deliberately Released Into The Environment written by Dan Funck Jensen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Microorganisms, including those genetically modified (GMOs), are increasingly being released deliberately into the environment for different purposes. One major interest is the use of antagonistic organisms for biological control of pests and plant diseases. Both in the development of biocontrol agents and their later risk assessment and product approval, there is a need for methods that facilitate monitoring of such introduced microorganisms. This book particularly addresses the various approaches for monitoring introduced fungi and the current status of techniques which may be relevant to use are described. Such techniques include marker genes and other DNA-based techniques, serology using polyclonals or monoclonals, the use of enzymes or secondary metabolite profiles, and `electronic noses' as well as more traditional methods like dilution plating.

Mycotoxins In Food

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Author : N Magan
language : en
Publisher: Elsevier
Release Date : 2004-07-16

Mycotoxins In Food written by N Magan and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-07-16 with Medical categories.

Mycotoxins, toxic compounds produced by fungi, pose a significant contamination risk in both animal feed and foods for human consumption. With its distinguished editors and international team of contributors, Mycotoxins in food summarises the wealth of recent research on how to assess the risks from mycotoxins, detect particular mycotoxins and control them at differing stages in the supply chain.Part one addresses risk assessment techniques, sampling methods, modelling and detection techniques used to measure the risk of mycotoxin contamination and the current regulations governing mycotoxin limits in food. Part two looks at how the risk of contamination may be controlled, with chapters on the use of HACCP systems and mycotoxin control at different stages in the supply chain. Two case studies demonstrate how these controls work for particular products. The final section details particular mycotoxins, from ochratoxin A and patulin to zearalenone and fumonisins.Mycotoxins in food is a standard reference for all those concerned with ensuring the safety of food. - Discusses the wealth of recent research in this important area - Covers risk assessment, detection of particular mycotoxins and how to control them throughout the supply chain - Describes how the risk of contamination can be controlled, including the use of HACCP systems

Root Physiology From Gene To Function

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Author : Hans Lambers
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-02-03

Root Physiology From Gene To Function written by Hans Lambers and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-03 with Science categories.

In the last decade, enormous progress has been made on the physiology of plant roots, including on a wide range of molecular aspects. Much of that progress has been captured in the chapters of this book. Breakthroughs have been made possible through integration of molecular and whole-plant aspects. The classical boundaries between physiology, biochemistry and molecular biology have vanished. There has been a strong focus on a limited number of model species, including Arabidopsis thaliana. That focus has allowed greater insight into the significance of specific genes for plant development and functioning. However, many species are very different from A. thaliana, in that they are mycorrhizal, develop a symbiosis with N2-fixing microsymbionts, or have other specialised root structures. Also, some have a much greater capacity to resist extreme environments, such as soil acidity, salinity, flooding or heavy-metal toxicities, due to specific adaptations. Research on species other than A. thaliana is therefore pivotal, to develop new knowledge in plant sciences in a comprehensive manner. This fundamental new knowledge can be the basis for important applications in, e.g., agriculture and plant conservation. Although significant progress has been made, much remains to be learnt. It is envisaged that discoveries made in the recent past will likely lead to major breakthroughs in the next decade.

Handbook Of Industrial Mycology

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Author : Zhiqiang An
language : en
Publisher: CRC Press
Release Date : 2004-08-30

Handbook Of Industrial Mycology written by Zhiqiang An and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08-30 with Science categories.

Several excellent books have been published that address one or more aspects of the diverse field of industrial mycology, but none of them cover the entire process of fungal bioactive metabolites discovery. Until now. The Handbook of Industrial Mycology provides, in one volume, an overview of recent developments in industrial mycology with emphasis on the discovery of bioactive metabolites and, most importantly, their underlying biology and genetics. Two additional features distinguish this book from other books in the field: 1) most chapters are prepared using experimental data to illustrate theories and 2) the authors provide methodologies and experimental protocols in their chapters. Presenting a comprehensive overview of recent advances, the book provides a framework of basic methods, tools, and organizational principles for channeling fungal germplasm into the academic, pharmaceutical, and enzyme discovery laboratories. It covers the complex range of processes involved in the discovery, characterization, and profiling of bioactive fungal metabolites. The book includes examples of several recently marketed fungal metabolites and explores the impact of fungi on applications in the pharmaceutical, food and beverage, agricultural, and agrochemical industries.

Patterns Function And Application Of Seed Microbiome

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Author : Mohammad Yaseen Mir
language : en
Publisher: Elsevier
Release Date : 2025-02-06

Patterns Function And Application Of Seed Microbiome written by Mohammad Yaseen Mir and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-06 with Science categories.

Patterns, Function and Application of Seed Microbiome: Bacteria, Fungi and Viruses presents the current understanding of seed and microbiota associations, emphasizing recent findings that highlight the latest insights into these interactions, and how they may ultimately influence plant ecology, health and productivity in both natural and agricultural systems. The development and dispersal of seeds and their transition to seedlings are among the most critical stages of the life cycle of plants and seed health is of crucial global importance for producing both quantity and high quality crops to feed world's population. It is important for seeds to be produced that contain the best hybrid embryo so that it is free of diseases, includes beneficial endophytes and ensures a healthy start of a seedling. Focusing on the role of seed microbiota, their transmission and implications for producing healthier seeds as well as the relationship between the seed microbiome and primed plant resistance this book reveals the targeted use of the seed microbiome to enrich the soil biodiversity and its resilience and its resulting impact on plant health and yield. also explores the importance of nanotechnology in the development and production of crops for both improved plant production, and plant protection. It explores the different microbes that are vectored onto and within the seeds of both cultivated and non-cultivated plants, with an emphasis on the importance and roles of seed microbiomes in sustainable agriculture. It explains how to change seeds to increase microbiome vectoring; repairing damaged ones, and conserves seed microbiota. It also present seed microbiome roles for plant health and design effective microbiome engineering. - Explores seed microbiome transmission and implications for producing healthier seeds - Includes modelling and meta-analysis of seed microbiome - Presents insights into the assembly and structure framework of seed microbiomes - Includes metagenomics and the potential of seed endophytes - Considers the seed microbiome as bio-control agents

Essentials Of Clinical Mycology

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Author : Carol A. Kauffman
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-01-12

Essentials Of Clinical Mycology written by Carol A. Kauffman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-01-12 with Medical categories.

Clinical Mycology offers a comprehensive review of this discipline. Organized by types of fungi, this volume covers microbiologic, epidemiologic and demographic aspects of fungal infections as well as diagnostic, clinical, therapeutic, and preventive approaches. Special patient populations are also detailed.