Automated Real Time Nucleic Acid Amplification Technology For Rapid And Simultaneous Detection Of Tuberculosis And Rifampicin Resistance Xpert Mtb Rif Assay For The Diagnosis Of Pulmonary And Extrapu
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Automated Real Time Nucleic Acid Amplification Technology For Rapid And Simultaneous Detection Of Tuberculosis And Rifampicin Resistance Xpert Mtb Rif Assay For The Diagnosis Of Pulmonary And Extrapu
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Author :
language : en
Publisher:
Release Date : 2013
Automated Real Time Nucleic Acid Amplification Technology For Rapid And Simultaneous Detection Of Tuberculosis And Rifampicin Resistance Xpert Mtb Rif Assay For The Diagnosis Of Pulmonary And Extrapu written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Fundamentals Of Microbiology
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Author : Jeffrey C. Pommerville
language : en
Publisher: Jones & Bartlett Publishers
Release Date : 2014
Fundamentals Of Microbiology written by Jeffrey C. Pommerville and has been published by Jones & Bartlett Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Medical categories.
Every new copy of the print book includes access code to Student Companion Website!The Tenth Edition of Jeffrey Pommerville's best-selling, award-winning classic text Fundamentals of Microbiology provides nursing and allied health students with a firm foundation in microbiology. Updated to reflect the Curriculum Guidelines for Undergraduate Microbiology as recommended by the American Society of Microbiology, the fully revised tenth edition includes all-new pedagogical features and the most current research data. This edition incorporates updates on infectious disease and the human microbiome, a revised discussion of the immune system, and an expanded Learning Design Concept feature that challenges students to develop critical-thinking skills.Accesible enough for introductory students and comprehensive enough for more advanced learners, Fundamentals of Microbiology encourages students to synthesize information, think deeply, and develop a broad toolset for analysis and research. Real-life examples, actual published experiments, and engaging figures and tables ensure student success. The texts's design allows students to self-evaluate and build a solid platform of investigative skills. Enjoyable, lively, and challenging, Fundamentals of Microbiology is an essential text for students in the health sciences.New to the fully revised and updated Tenth Edition:-New Investigating the Microbial World feature in each chapter encourages students to participate in the scientific investigation process and challenges them to apply the process of science and quantitative reasoning through related actual experiments.-All-new or updated discussions of the human microbiome, infectious diseases, the immune system, and evolution-Redesigned and updated figures and tables increase clarity and student understanding-Includes new and revised critical thinking exercises included in the end-of-chapter material-Incorporates updated and new MicroFocus and MicroInquiry boxes, and Textbook Cases-The Companion Website includes a wealth of study aids and learning tools, including new interactive animations**Companion Website access is not included with ebook offerings.
Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis
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Author : Ka-Lun Wong
language : en
Publisher:
Release Date : 2017-01-26
Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis written by Ka-Lun Wong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-26 with categories.
This dissertation, "Development of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Tuberculosis" by Ka-lun, Wong, 王嘉倫, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Tuberculosis (TB), a severe disease caused by Mycobacteria tuberculosis (MTb), remains a globally severe health problem. In modern days, emergence of drug resistant TB is a new threat to public health since it can lead to treatment failure, increased transmission of TB to other hosts and further development of drug resistant complications. The traditional diagnostic method for TB by Acid Fast Bacilli (AFB) smear and Lowenstein-Jensen medium (LJ) culture are poor in sensitivity and time consuming respectively. There is a need for rapid diagnosis and identification of MTb. Nucleic acid amplification like PCR and real-time PCR are options for rapid diagnosis. However, such techniques require sophisticated technique and complex equipment. The high cost would constitute a barrier for countries with a high demand but only limited resources. Loop-mediated isothermal amplification (LAMP) assay is a novel technique, proven by many studies as to its high sensitivity and highly specificity to MTb. Most importantly, LAMP assay is economical and affordable by developing countries. The first objective of this study was to evaluate the analytical sensitivity and specificity of LAMP assay. The specificity of LAMP assay was determined by performing LAMP assay on 19 clinical isolates, which had already been identified previously. The clinical isolates included 14 mycobacteria tuberculosis complex (MTb), and five mycobacteria other than tuberculosis (MOTT) strains that were positive for AFB smear and LJ culture but negative for IS6110 single-tube nested real time PCR. The specificity was 100%. The analytical sensitivity as well as the limit of detection (LOD) were determined by testing on a duplicate set of serial DNA dilution, where each duplicate consisted of dilution of 100,000, 10,000, 1000, 300, 100, 10 and 1 colony forming unit/milliliter (CFU/ml). The LOD of LAMP assay was about 3 CFU per reaction. The second objective of this study evaluated the diagnostic performance of LAMP assay against AFB culture and IS6110 single tube nested real time PCR for identification of MTb in 200 respiratory specimens from 123 patients. All the specimens have already been tested for IS6110 single tube nested real time PCR, and culture results and AFB smears results have been obtained for all the specimens. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of three diagnostic methods (AFB smear microscopy, LAMP assay amplification and IS6110 single-tube nested real time PCR) were calculated with 95% confidence interval using LJ culture as gold standard. The LAMP assay had a sensitivity of 80%, specificity of 92.6%, PPV of 60% and NPV of 97.1%. However, MTb might fail to grow on the LJ culture medium for various reasons, for instance, MTb might already be dead after antibiotic treatment of the patient, or there might be poor laboratory practices during the processing of the specimens. Since the LJ culture method could produce false negatives in the situations described above, an alternative to the LJ culture method, ''Hybrid Method'' was used as the gold standard. Under this method, a specimen was regarded as positive if the LJ culture result was positive. On the other hand, if a specimen generated a negative result using the LJ culture method, the results from the LAMP assay and IS6110 nested real time PCR would be considered, i.e., if both the LAMP assay