Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis
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Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis
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Author : 王嘉倫
language : en
Publisher:
Release Date : 2013
Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis written by 王嘉倫 and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Gene amplification categories.
Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis
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Author : Ka-Lun Wong
language : en
Publisher:
Release Date : 2017-01-26
Development Of Loop Mediated Isothermal Amplification Assay For Rapid Diagnosis Of Tuberculosis written by Ka-Lun Wong and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-01-26 with categories.
This dissertation, "Development of Loop-mediated Isothermal Amplification Assay for Rapid Diagnosis of Tuberculosis" by Ka-lun, Wong, 王嘉倫, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Tuberculosis (TB), a severe disease caused by Mycobacteria tuberculosis (MTb), remains a globally severe health problem. In modern days, emergence of drug resistant TB is a new threat to public health since it can lead to treatment failure, increased transmission of TB to other hosts and further development of drug resistant complications. The traditional diagnostic method for TB by Acid Fast Bacilli (AFB) smear and Lowenstein-Jensen medium (LJ) culture are poor in sensitivity and time consuming respectively. There is a need for rapid diagnosis and identification of MTb. Nucleic acid amplification like PCR and real-time PCR are options for rapid diagnosis. However, such techniques require sophisticated technique and complex equipment. The high cost would constitute a barrier for countries with a high demand but only limited resources. Loop-mediated isothermal amplification (LAMP) assay is a novel technique, proven by many studies as to its high sensitivity and highly specificity to MTb. Most importantly, LAMP assay is economical and affordable by developing countries. The first objective of this study was to evaluate the analytical sensitivity and specificity of LAMP assay. The specificity of LAMP assay was determined by performing LAMP assay on 19 clinical isolates, which had already been identified previously. The clinical isolates included 14 mycobacteria tuberculosis complex (MTb), and five mycobacteria other than tuberculosis (MOTT) strains that were positive for AFB smear and LJ culture but negative for IS6110 single-tube nested real time PCR. The specificity was 100%. The analytical sensitivity as well as the limit of detection (LOD) were determined by testing on a duplicate set of serial DNA dilution, where each duplicate consisted of dilution of 100,000, 10,000, 1000, 300, 100, 10 and 1 colony forming unit/milliliter (CFU/ml). The LOD of LAMP assay was about 3 CFU per reaction. The second objective of this study evaluated the diagnostic performance of LAMP assay against AFB culture and IS6110 single tube nested real time PCR for identification of MTb in 200 respiratory specimens from 123 patients. All the specimens have already been tested for IS6110 single tube nested real time PCR, and culture results and AFB smears results have been obtained for all the specimens. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of three diagnostic methods (AFB smear microscopy, LAMP assay amplification and IS6110 single-tube nested real time PCR) were calculated with 95% confidence interval using LJ culture as gold standard. The LAMP assay had a sensitivity of 80%, specificity of 92.6%, PPV of 60% and NPV of 97.1%. However, MTb might fail to grow on the LJ culture medium for various reasons, for instance, MTb might already be dead after antibiotic treatment of the patient, or there might be poor laboratory practices during the processing of the specimens. Since the LJ culture method could produce false negatives in the situations described above, an alternative to the LJ culture method, ''Hybrid Method'' was used as the gold standard. Under this method, a specimen was regarded as positive if the LJ culture result was positive. On the other hand, if a specimen generated a negative result using the LJ culture method, the results from the LAMP assay and IS6110 nested real time PCR would be considered, i.e., if both the LAMP assay
Evolution Genomic Adaptation Of Emerging And Re Emerging Rna Viruses
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Author : Kai Huang
language : en
Publisher: Frontiers Media SA
Release Date : 2022-01-06
Evolution Genomic Adaptation Of Emerging And Re Emerging Rna Viruses written by Kai Huang and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-06 with Science categories.
Current Advancements In Real Time Plant Pathogen Diagnostics From Lab Assays To In Field Detection
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Author : Ravinder Kumar
language : en
Publisher: Frontiers Media SA
Release Date : 2023-08-31
Current Advancements In Real Time Plant Pathogen Diagnostics From Lab Assays To In Field Detection written by Ravinder Kumar and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-08-31 with Science categories.
Evolving Landscape Of Molecular Diagnostics
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Author : Mrutyunjay Suar
language : en
Publisher: Elsevier
Release Date : 2024-06-27
Evolving Landscape Of Molecular Diagnostics written by Mrutyunjay Suar and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-27 with Science categories.
Evolving Landscape of Molecular Diagnostics: Applications and Techniques presents cutting-edge reviews on current and emerging technologies in the diagnosis of microbial infections. The book discusses the fundamentals of Molecular Diagnostics for bacteria, fungi, viruses and a variety of state-of-the-art diagnostics tools and techniques for obtaining qualitative and quantitative results. It includes topics such as Next generation sequencing and application of "OMICS" for early disease diagnosis. Sections cover the entire spectrum of emerging diagnostic tools and techniques, as well as the principles, pros and cons of each method and applications for diagnosis of infectious disease in plants, humans and veterinary. This is a resource for both researchers and students working in clinical microbiology, infectious biology, applied life sciences and scientists working in the clinical diagnostic industry. - Provides a wide range of diagnostics tools and technologies in the field of infectious biology - Presents discussions on emerging technologies for early disease diagnosis and novel diagnostics techniques for detection of COVID, Urea Breath Tests for Detection of Helicobacter pylori, Loop-Mediated Isothermal Amplification as Point-of-Care Diagnosis, Molecular imaging, Lab-on-a-chip Technologies, and several others - Analyzes literature reviews, underpinning methodologies, and opportunities and limitations - Includes case studies and examples that highlight applications using the underpinning techniques
Handbook Of Global Tuberculosis Control
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Author : Yichen Lu
language : en
Publisher: Springer
Release Date : 2017-06-09
Handbook Of Global Tuberculosis Control written by Yichen Lu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-09 with Medical categories.
This ambitious reference surveys worldwide efforts at controlling the spread of tuberculosis, with special emphasis on the developing world. Case studies from China, Pakistan, Nigeria, Indonesia, and other frontline countries demonstrate a wealth of information on clinical, cultural, socioeconomic, and other relevant factors. This compilation provides a valuable resource for creating successful intervention and prevention strategies. State-of-the-science snapshots pinpoint where short- and long-term initiatives stand today, from early detection and vaccination programs to new genetic technologies and drug therapies. This diverse group of perspectives and approaches offers innovative paths toward control and realistic odds for containing the threat, especially in the face of current co-epidemics and new drug-resistant strains. Among the topics in the Handbook: Diagnosis of tuberculosis: current pipeline, unmet needs, and new developments Concurrence of tuberculosis and other major diseases The tuberculosis outbreak response, investigation, and control The promise of new TB vaccines DNA fingerprinting of Mycobacterium tuberculosis: a rich source of fundamental and daily applicable knowledge Global tuberculosis surveillance The Handbook of Global Tuberculosis Control is urgent reading for leadership and staff of non-governmental organizations, government agencies, academic institutions, research centers, hospitals, and potentially businesses with interests in tuberculosis control. Additionally, the book's focus on TB in developing countries will attract a wider audience of practitioners, particularly those working in the broader fields of global public health, epidemiology, international development, and the socioeconomics of infectious diseases.
The Role Of New Technologies In Medical Microbiological Research And Diagnosis
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Author : John P. Hays
language : en
Publisher: Bentham Science Publishers
Release Date : 2012
The Role Of New Technologies In Medical Microbiological Research And Diagnosis written by John P. Hays and has been published by Bentham Science Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with Medical categories.
This e-book provides a comprehensive overview of state of the art applications of biomolecular techniques that are currently used, or are in development in the field of microbiological diagnostics research. In this respect, the topics covered include, genomics, proteomics, immunologics, biosensors, microarrays and nano-culture technologies. The broad range of techniques covered by the book will be invaluable to readers from professions allied to (but not exclusive to) microbiology analysts and researchers, laboratory technicians, (medical) microbiologists, molecular biologists, analytical phys.
Pcr Technology
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Author : Tania Nolan
language : en
Publisher: CRC Press
Release Date : 2013-06-13
Pcr Technology written by Tania Nolan and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-13 with Law categories.
PCR's simplicity as a molecular technique is, in some ways, responsible for the huge amount of innovation that surrounds it, as researchers continually think of new ways to tweak, adapt, and re-formulate concepts and applications. PCR Technology: Current Innovations, Third Edition is a collection of novel methods, insights, and points of view that
Tuberculosis E Book
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Author : H. Simon Schaaf
language : en
Publisher: Elsevier Health Sciences
Release Date : 2009-03-24
Tuberculosis E Book written by H. Simon Schaaf and has been published by Elsevier Health Sciences this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-24 with Medical categories.
This book provides all the vital information you need to know about tuberculosis, especially in the face of drug-resistant strains of the disease. Coverage includes which patient populations face an elevated risk of infection, as well as which therapies are appropriate and how to correctly monitor ongoing treatment so that patients are cured. Properly administer screening tests, interpret their results, and identify manifestations of the disease, with authoritative guidance from expert clinicians from around the world. Discusses screening tests for tuberculosis so you can interpret their results and identify not only common manifestations of the disease, but also those that are comparatively rare—such as tuberculosis in pregnant women. Covers all clinical aspects of tuberculosis in children, including current practices on managing those infected with HIV. Provides details on how best to interact with the public health system in both industrialized and developing countries. Addresses the social aspects of tuberculosis and presents the latest advances on new and potential vaccines against tuberculosis. Offers the expertise of internationally recognized tuberculosis clinicians to provide you with well-rounded, global coverage. Features numerous illustrations to provide clear and detailed depictions of rare manifestations of tuberculosis.
Diagnosis Of Mycobacterium
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Author : Amit Singh
language : en
Publisher: Springer Nature
Release Date : 2023-11-14
Diagnosis Of Mycobacterium written by Amit Singh and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-14 with Science categories.
This book covers all the aspects related to the laboratory diagnosis of Mycobacterium. Laboratory diagnosis includes microscopic to advance molecular diagnosis. Moreover, it deals with the methods used to detect antimycobacterial susceptibility tests and drug resistance. Chapters cover most potential methods and techniques from traditional to advanced future diagnostics for identifying tuberculosis, NTM, and latent TB infections. Also discussed the challenges and opportunities in the diagnostic science of infectious disease. This book offers assistance to students, postgraduate medical students, researchers, medical practitioners, postdoctoral fellows, microbiologists, nurses, and public health officers working in tuberculosis control programs to understand various modalities for diagnosis and drug-resistance tuberculosis.