Baculovirus Expression Systems And Biopesticides
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Baculovirus Expression Systems And Biopesticides
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Author : Michael L. Shuler
language : en
Publisher: Wiley-Liss
Release Date : 1995
Baculovirus Expression Systems And Biopesticides written by Michael L. Shuler and has been published by Wiley-Liss this book supported file pdf, txt, epub, kindle and other format this book has been release on 1995 with Science categories.
Developing effective baculovirus-insect culture systems. Insect cell culture methods and their use in virus research. Comparison of mammalian and insect cell cultures. protein production and processing from baculovirus. Development and testing of genetically improved baculovirus insecticides. Fundamentals of baculovirus-insect cell attachment and infection. Development and evaluation of host insect cells. Bioreactor design and scale-up issues. The effect of hydrodynamic forces on insect cells. Commercial application of insect cell culture. Baculovirus-mediated production of proteins in insect cells. Potential application of insect cell-based expression systems in the bio/pharmaceutical industry.
Baculovirus Expression Vectors
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Author : David R. O'Reilly
language : en
Publisher:
Release Date : 1994
Baculovirus Expression Vectors written by David R. O'Reilly and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994 with Baculoviruses categories.
Baculoviruses have proven to be the most powerful and versatile eukaryotic expression vectors available. This unique laboratory manual is designed to help both beginning and experienced researchers construct and use baculovirus vector systems. It simplifies selection of the most appropriatebaculovirus vector design for a given problem, then describes each step of the implementation process--from vector construction to large-scale protein production. The book provides an understanding of how the vectors work; a biological overview of cells, viruses, plasmids, and promoters; guidelinesfor choosing optimum vectors; protocols for growing insect cells and recombinant viruses; methods of analyzing protein products and scaling up protein production; techniques for producing proteins in insect larvae; and easy-to-use maps charting available expression vectors. This comprehensiveapproach has many benefits for researchers and students alike. It allows them to understand how and why the vector system works and offers a rapid comparison of options for choosing the right virus, plasmid or promoter for vector design and construction, with a minimum amount of lost time. Themanual is an invaluable resource for every individual engaged in the production of proteins for any purpose.
The Baculovirus Expression System
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Author : Linda King
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
The Baculovirus Expression System written by Linda King and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The decision to write a book about the practical aspects of the baculovirus expression system stems from the numerous phone calls for help we have had, and from the many visitors to our labora tories requiring assistance to find the elusive polyhedrin-negative virus containing their favourite gene. We have also organized two expression system workshops and from the manuals we wrote for these, it seemed a logical progression to extend them into book form. We appreciate that those who are 'old-hands' at the baculovirus expression system may have differing views on some of our procedures, but the methods in this book are presented in the light of our own experiences in the laboratory and from our practical workshops, and we hope that the book will be especially useful to those new to the system. The first three chapters give the background information to the baculovirus expression system, and includes advice on how to choose the right transfer vector and discusses the various methods that are available to select recombinant viruses. The practical chapters concentrate on those aspects which are novel to the baculovirus system (insect cell culture, virus amplification and titration, etc. ) and, in general, leave the standard molecular biological techniques to the other excellent laboratory manuals that are available. However, for completeness sake and to avoid constant reference to other manuals, we have included brief details of some standard techniques where they are integral to the success of the baculovirus protocols.
Baculovirus Expression Protocols
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Author : Christopher D. Richardson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Baculovirus Expression Protocols written by Christopher D. Richardson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
Baculovirus Expression Protocols offers both industrial and university-based researchers a comprehensive comilation of the latest baculovirus techniques along with step-by-step instructions and time-saving techniques. The contributors-leading authorities in the field-present the assorted expression plasmids currently in use, guide the reader through the process of generating and selecting recombinant virus, and describe specific examples of recombinant protein production and purification. The emphasis is on alternative and simpler screening techniques for the selection of recombinant baculovirus. The book also surveys the various insect cell lines currently compatible with the baculovirus system. Highlights include production of recombinant virus using linearized DNA and vectors that contain a b-galactosidase indicator and a complete list of expression vectors currently available. Detailed descriptions for the scale-up of protein production using spinner flasks, bioreactors, and insect larvae are also included. Baculovirus Expression Protocols offers both industrial and university-based researchers an outstanding collection of reproducible, step-by-step laboratory protocols. It will immediately become indispensable for anyone working with baculoviruses and their application in the expression of recombinant proteins in insect cells.
Insect Viruses
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Author : Byrony C Bonning
language : en
Publisher: Academic Press
Release Date : 2006-09-22
Insect Viruses written by Byrony C Bonning and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-09-22 with Science categories.
Baculoviruses are perhaps unique among viruses in the breadth of their biotechnological applications: these insect specific viruses are used not only for insect pest management purposes, but also as laboratory research tools for production of recombinant proteins and for protein display, and as potential vectors for human gene therapy. In addition to highlighting recent advances, this volume provides a comprehensive review of the biotechnological applications of these and other insect viruses in both the academic and private sectors.
Baculovirus And Insect Cell Expression Protocols
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Author : David W. Murhammer
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-04-15
Baculovirus And Insect Cell Expression Protocols written by David W. Murhammer and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-04-15 with Medical categories.
The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.
Microbial Biopesticides
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Author : Opender Koul
language : en
Publisher: CRC Press
Release Date : 2001-10-25
Microbial Biopesticides written by Opender Koul and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-10-25 with Science categories.
Biotechnological research has provided key developments in pest control agents, focusing on pathogens of insect pests as formulated biological pesticides. Emphasis has been placed on bacteria and viruses as they are well understood and easily manipulated. Microbial Biopesticides provides a comprehensive overview of the advances made in the use of b
Antibody Expression And Production
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Author : Mohamed Al-Rubeai
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-05-16
Antibody Expression And Production written by Mohamed Al-Rubeai and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-16 with Medical categories.
Engineered antibodies currently represent over 30% of biopharmaceuticals in clinical trials and their total worldwide sales continue to increase significantly. The importance of antibody applications is reflected in their increasing clinical and industrial applications as well as in the progression of established and emerging production strategies. This volume provides detailed coverage of the generation, optimization, characterization, production and applications of antibody. It provides the necessary theoretical background and description of methods for the expression of antibody in microbial and animal cell cultures and in transgenic animals and plants. There is a strong focus on those issues related to the production of intrabodies, bispecific antibody and antibody fragments and also to novel applications in cancer immunotherapy.
Glycotechnology
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Author : E. G. Berger
language : en
Publisher: Springer
Release Date : 2012-10-14
Glycotechnology written by E. G. Berger and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-14 with Science categories.
Glycotechnology brings together in one place important contributions and up-to-date research results in this fast moving area. Glycotechnology serves as an excellent reference, providing insight into some of the most challenging research issues in the field.
Insect Cell Cultures
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Author : Just M. Vlak
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-04-11
Insect Cell Cultures written by Just M. Vlak and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-04-11 with Science categories.
A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.