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Biological Low Voltage Scanning Electron Microscopy

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Biological Low Voltage Scanning Electron Microscopy

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Author : James Pawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-03

Biological Low Voltage Scanning Electron Microscopy written by James Pawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-03 with Science categories.

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Biological Field Emission Scanning Electron Microscopy 2 Volume Set

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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-04-29

Biological Field Emission Scanning Electron Microscopy 2 Volume Set written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-29 with Science categories.

The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Image Formation In Low Voltage Scanning Electron Microscopy

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Author : Ludwig Reimer
language : en
Publisher: SPIE Press
Release Date : 1993

Image Formation In Low Voltage Scanning Electron Microscopy written by Ludwig Reimer and has been published by SPIE Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Science categories.

While most textbooks about scanning electron microscopy (SEM) cover the high-voltage range from 5-50 keV, this volume considers the special problems in low-voltage SEM and summarizes the differences between LVSEM and conventional SEM. Chapters cover the influence of lens aberrations and design on electron-probe formation; the effect of elastic and inelastic scattering processes on electron diffusion and electron range; charging and radiation damage effects; the dependence of SE yield and the backscattering coefficient on electron energy, surface tilt, and material as well as the angular and energy distributions; and types of image contrast and the differences between LVSEM and conventional SEM modes due to the influence of electron-specimen interactions.

Low Voltage Electron Microscopy

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Author : David C. Bell
language : en
Publisher: John Wiley & Sons
Release Date : 2013-02-11

Low Voltage Electron Microscopy written by David C. Bell and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-02-11 with Science categories.

Part of the Wiley-Royal Microscopical Society Series, this book discusses the rapidly developing cutting-edge field of low-voltage microscopy, a field that has only recently emerged due to the rapid developments in the electron optics design and image processing. It serves as a guide for current and new microscopists and materials scientists who are active in the field of nanotechnology, and presents applications in nanotechnology and research of surface-related phenomena, allowing researches to observe materials as never before.

Biological Field Emission Scanning Electron Microscopy

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Author : Roland A. Fleck
language : en
Publisher: John Wiley & Sons
Release Date : 2019-02-06

Biological Field Emission Scanning Electron Microscopy written by Roland A. Fleck and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-06 with Science categories.

Scanning Electron Microscopy For The Life Sciences

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Author : Heide Schatten
language : en
Publisher: Cambridge University Press
Release Date : 2013

Scanning Electron Microscopy For The Life Sciences written by Heide Schatten and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with Science categories.

A guide to modern scanning electron microscopy instrumentation, methodology and techniques, highlighting novel applications to cell and molecular biology.

Multidimensional Microscopy

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Author : P.-C. Cheng
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Multidimensional Microscopy written by P.-C. Cheng and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Modern cell biology is being revolutionized by the wedding of microscopy and computers. This book describes the new instrumentation and methods which allow three-dimensional reconstruction of specimens. Multidimensional Microscopy will be of interest to cell biologists, microscopists, and basic biomedical researchers whose work involves microscopic techniques. This book presents current results on a very active field in modern biology: methods in light and electron microscopy that allow the reconstruction of three-dimensional objects with the aid of computers. The book emphasizes the methods that can be used and examples of biological systems to which they have been applied. It includes extensive descriptions of confocal microscopy and its applications, as well as chapters on X-ray microscopy, low-voltage electron microscopy, and image reconstruction. This is an impressive summary of state-of-the-art methods in microscopy, in which microscopes and computers are being joined to permit specimens to be examined and reconstructed in three dimensions. Will be of interest to cell biologists, biomedical researchers, and microscopists.

Principles And Practice Of Variable Pressure Environmental Scanning Electron Microscopy Vp Esem

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Author : Debbie Stokes
language : en
Publisher: John Wiley & Sons
Release Date : 2008-11-20

Principles And Practice Of Variable Pressure Environmental Scanning Electron Microscopy Vp Esem written by Debbie Stokes and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-20 with Science categories.

Offers a simple starting point to VPSEM, especially for new users, technicians and students containing clear, concise explanations Crucially, the principles and applications outlined in this book are completely generic: i.e. applicable to all types of VPSEM, irrespective of manufacturer. Information presented will enable reader to turn principles into practice Published in association with the Royal Microscopical Society (RMS) -www.rms.org.uk

Advances In Electronics And Electron Physics

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Author :
language : en
Publisher: Academic Press
Release Date : 1992-08-17

Advances In Electronics And Electron Physics written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-08-17 with Computers categories.

Advances in Electronics and Electron Physics

Colloidal Gold

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Author :
language : en
Publisher: Elsevier
Release Date : 2012-12-02

Colloidal Gold written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Technology & Engineering categories.

Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. - Among the first books to cover principles and methodology of colloidal gold in microscopy - Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book - Discusses problems and limitations of techniques - Guides users to avoid problems and choose the correct procedures for specific applications - Contributors are eminent authorities in their fields - Topics covered in Volume 3 include: Production of monoclonal and polyclonal antibodies, Lowicryl, polar, and apolar resins - Preserving and localizing antigenicity; sectioning; high-resolution morphological localization - Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation - Applications to biomedical studies - Colloidal gold in conjunction with video-enhanced microscopy

Biological Low Voltage Scanning Electron Microscopy

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