Biomolecular Patterning In Microfabricated Systems With Avidin Biotin And Microcontact Printing
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Biomolecular Patterning In Microfabricated Systems With Avidin Biotin And Microcontact Printing
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Author : Reid Nelson Orth
language : en
Publisher:
Release Date : 2001
Biomolecular Patterning In Microfabricated Systems With Avidin Biotin And Microcontact Printing written by Reid Nelson Orth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Biomolecular Patterning On Micro And Nanofabricated Surfaces For Biosensor Applications And Cell Based Research
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Author : Reid Nelson Orth
language : en
Publisher:
Release Date : 2003
Biomolecular Patterning On Micro And Nanofabricated Surfaces For Biosensor Applications And Cell Based Research written by Reid Nelson Orth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003 with categories.
In this study, biomaterials are patterned at the micro- and nanometer scale for biosensor applications and cell-based research. Two enabling technologies were photoactivatable biotin and the polymer lift-off technique. Avidin-biotin complexes have been immobilized on planar silicon substrates and to the inner surfaces of capillary tubes. The latter serves as a model for patterning inside microfluidic systems. Several biomaterials have been patterned using the polymer lift-off method, including lipids, metal, microspheres, biotin, and proteins (e.g., antibodies, protein A, and NeutrAvidin). The patterned lipids formed supported lipid bilayers with diffusion coefficients comparable to cell membranes as confirmed by fluorescence recovery after photobleaching (FRP). The author's tailored patterns comprised 2, 4-dinitrophenyl (DNP) or biotin functionalized lipids with features ranging from 1.3 micrometers to 76 micrometers. Non-lipid molecules were patterned in features as small as 700 nanometers. A characterization of the surfaces has shown a highly uniform patterning. Several of these materials also were applied in patterned regions inside microtrenches. Micro- and nanometer-scale patterns of functionalized biomaterials served as spatially controlled stimuli for cell surface ligands in this research. This technology has been applied to rat basophilic leukocyte (RBL) cells, eosinophils, lymphocytes, macrophages, and fibroblasts. For RBL experiments, DNP-conjugated lipids have been incorporated in the lipid bilayers with fluorescent lipid DiIC16 and used to stimulate RBL cells sensitized with anti-DNP IgE on the Fc receptors. Receptor aggregation was observed with confocal microscopy. This technique allows researchers to study the effects of antigen density on cellular response, binding kinetics, and the redistribution after individual binding events. (1 table, 71 figures).
Biomaterial Based Approaches To Study The Tumour Microenvironment
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Author : Jessica O Winter
language : en
Publisher: Royal Society of Chemistry
Release Date : 2022-12-07
Biomaterial Based Approaches To Study The Tumour Microenvironment written by Jessica O Winter and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-07 with Technology & Engineering categories.
The tumour microenvironment is increasingly recognized as an important contributor to cancer progression and treatment. However, most cancer studies continue to be performed in 2D tissue culture dishes that do not capture the characteristics of the tumour niche. This book provides an introduction to the rich chemical, topographical, and mechanical cues in the tumour microenvironment and then introduces readers to bioengineering strategies, including scaffold design and synthesis, chemical signalling and delivery, and co-culture, microfluidics, and organ-on-a-chip tools that can be used to mimic tumour microenvironment features. This book also includes discussion of emerging imaging methods compatible with tumour microenvironment mimicking biomaterials and discusses applications of such models in immuno-oncology, metastasis, and drug screening. Edited by two leaders in the field, this book will appeal to graduate students and researchers working in biomaterials science, chemical and biomedical engineering departments.
Microfabrication Of Stimuli Responsive Polymers
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Author : Chuanliang Feng
language : en
Publisher: Springer Nature
Release Date : 2021-03-08
Microfabrication Of Stimuli Responsive Polymers written by Chuanliang Feng and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-03-08 with Technology & Engineering categories.
This book introduces readers to interfacial reactions in confinement on stimuli-responsive homopolymer and diblock copolymer films. It also includes investigations concerning the immobilization of (bio)molecules and the fabrication of biomolecular patterns by reactive microcontact printing on these reactive polymer films. In turn, the book takes advantage of the microphase separation of diblock copolymer films to study the fabrication of nanopatterns, which could contribute to the future development of a model system that allows us to area-selectively deposit and address (bio)molecules. Given its scope, the book broadens readers’ perspective on the microfabrication of stimuli-responsive polymers.
Conducting Polymers Fundamentals And Applications
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Author : Prasanna Chandrasekhar
language : en
Publisher: Springer
Release Date : 2018-02-28
Conducting Polymers Fundamentals And Applications written by Prasanna Chandrasekhar and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-02-28 with Technology & Engineering categories.
The second edition of this popular textbook thoroughly covers the practical basics and applications of conducting polymers. It also addresses materials that have gained prominence since the first edition of this book was published, namely carbon nanotubes and graphene. The features of this new edition include: New and updated chapters on novel concepts in conducting polymers Details on interdisciplinary applications of conducting polymers An in depth description of classes of conducting polymers
Biomems And Biomedical Nanotechnology
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Author : Mihrimah Ozkan
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-04-03
Biomems And Biomedical Nanotechnology written by Mihrimah Ozkan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-04-03 with Technology & Engineering categories.
Contributions reporting on fundamental and applied investigations of the material science, biochemistry, and physics of biomedical microdevices with applications to Genomics and Proteomics. Topics include gene expression profiling utilizing microarray technology; imaging and sensing for gene detection and use in DNA analysis; and coverage of advanced microfluidic devices and the Humane Genome Project.
Microfluidics For Single Cell Analysis
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Author : Jin-Ming Lin
language : en
Publisher: Springer Nature
Release Date : 2019-08-28
Microfluidics For Single Cell Analysis written by Jin-Ming Lin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with Science categories.
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Nanobioengineering
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Author : Bhupinder Singh
language : en
Publisher: CRC Press
Release Date : 2018-11-02
Nanobioengineering written by Bhupinder Singh and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-02 with Science categories.
The objective of this book is to provide the fundamental comprehension of a broad range of topics in an integrated volume such that readership hailing from diverse disciplines can rapidly acquire the necessary background for applying it in pertinent research and development field.
Combinatorial Methods For Chemical And Biological Sensors
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Author : Radislav A. Potyrailo
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-03-21
Combinatorial Methods For Chemical And Biological Sensors written by Radislav A. Potyrailo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-21 with Science categories.
Chemical sensors are in high demand for applications as varied as water pollution detection, medical diagnostics, and battlefield air analysis. Designing the next generation of sensors requires an interdisciplinary approach. The book provides a critical analysis of new opportunities in sensor materials research that have been opened up with the use of combinatorial and high-throughput technologies, with emphasis on experimental techniques. For a view of component selection with a more computational perspective, readers may refer to the complementary volume of Integrated Analytical Systems edited by M. Ryan et al., entitled “Computational Methods for Sensor Material Selection”.
Introduction To Biomems
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Author : Albert Folch
language : en
Publisher: CRC Press
Release Date : 2016-04-19
Introduction To Biomems written by Albert Folch and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-19 with Medical categories.
The entire scope of the BioMEMS field-at your fingertipsHelping to educate the new generation of engineers and biologists, Introduction to BioMEMS explains how certain problems in biology and medicine benefit from and often require the miniaturization of devices. The book covers the whole breadth of this dynamic field, including classical microfabr