Microfluidics For Single Cell Analysis

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Microfluidics For Single Cell Analysis
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Author : Jin-Ming Lin
language : en
Publisher: Springer Nature
Release Date : 2019-08-28
Microfluidics For Single Cell Analysis written by Jin-Ming Lin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with Science categories.
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Microfluidics For Single Cell Analysis
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Author :
language : en
Publisher:
Release Date : 2019
Microfluidics For Single Cell Analysis written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Cytology categories.
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor's laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Biosensors For Single Cell Analysis
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Author : Jian Chen
language : en
Publisher: Elsevier
Release Date : 2021-10-28
Biosensors For Single Cell Analysis written by Jian Chen and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-28 with Science categories.
Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization Features case studies that illustrate the application of biosensors for single-cell analysis Considers microfluidic approaches for each single-cell property discussed Explores future directions for single-cell analysis and biosensor technology
Open Space Microfluidics
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Author : Emmanuel Delamarche
language : en
Publisher: John Wiley & Sons
Release Date : 2018-01-18
Open Space Microfluidics written by Emmanuel Delamarche and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Technology & Engineering categories.
Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.
Microfluidics
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Author : Yu Song
language : en
Publisher: John Wiley & Sons
Release Date : 2018-05-07
Microfluidics written by Yu Song and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-07 with Science categories.
The first book offering a global overview of fundamental microfluidics and the wide range of possible applications, for example, in chemistry, biology, and biomedical science. As such, it summarizes recent progress in microfluidics, including its origin and development, the theoretical fundamentals, and fabrication techniques for microfluidic devices. The book also comprehensively covers the fluid mechanics, physics and chemistry as well as applications in such different fields as detection and synthesis of inorganic and organic materials. A useful reference for non-specialists and a basic guideline for research scientists and technicians already active in this field or intending to work in microfluidics.
Nanotechnology For Microfluidics
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Author : Xingyu Jiang
language : en
Publisher: John Wiley & Sons
Release Date : 2020-09-08
Nanotechnology For Microfluidics written by Xingyu Jiang and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09-08 with Technology & Engineering categories.
The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.
Droplet Microfluidics
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Author : Carolyn Ren
language : en
Publisher: Royal Society of Chemistry
Release Date : 2020-11-20
Droplet Microfluidics written by Carolyn Ren and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-11-20 with Science categories.
Droplet microfluidics offers tremendous potential as an enabling technology for high-throughput screening. It promises to yield novel techniques for personalised medicine, drug discovery, disease diagnosis, establishing chemical libraries, and the discovery of new materials. Despite the enormous potential to contribute to a broad range of applications, the expected adoption has not yet been seen, partly due to the interdisciplinary nature and the fact that, up until now, information has been scattered across the literature. This book goes a long way to addressing these issues. Edited by two leaders, this book has drawn together expertise from around the globe to form a unified, cohesive resource for the droplet microfluidics community. Starting with the basic theory of droplet microfluidics before introducing its use as a tool, the reader will be treated to chapters on important techniques, including robust passive and active droplet manipulations and applications such as single cell analysis, which is key for drug discovery. This book is a go-to resource for the community yearning to adopt and promote droplet microfluidics into different applications and will interest researchers and practitioners working across chemistry, biology, physics, materials science, micro- and nano-technology, and engineering.
Functional Metagenomics Tools And Applications
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Author : Trevor C. Charles
language : en
Publisher: Springer
Release Date : 2017-10-23
Functional Metagenomics Tools And Applications written by Trevor C. Charles and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-23 with Science categories.
In this book, the latest tools available for functional metagenomics research are described.This research enables scientists to directly access the genomes from diverse microbial genomes at one time and study these “metagenomes”. Using the modern tools of genome sequencing and cloning, researchers have now been able to harness this astounding metagenomic diversity to understand and exploit the diverse functions of microorganisms. Leading scientists from around the world demonstrate how these approaches have been applied in many different settings, including aquatic and terrestrial habitats, microbiomes, and many more environments. This is a highly informative and carefully presented book, providing microbiologists with a summary of the latest functional metagenomics literature on all specific habitats.
Fundamentals Of Microfluidics And Lab On A Chip For Biological Analysis And Discovery
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Author : Paul C.H. Li
language : en
Publisher: CRC Press
Release Date : 2010-02-24
Fundamentals Of Microfluidics And Lab On A Chip For Biological Analysis And Discovery written by Paul C.H. Li and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-02-24 with Medical categories.
Lab-on-a-chip technology permits us to make many important discoveries that can only be observed at the microscale or the nanoscale. Using this technology, biological and biochemical analyses translate into greater sensitivity, more accurate results, and more valuable findings. Authored by one of the field's pioneering researchers, Fundamentals of
Encyclopedia Of Microfluidics And Nanofluidics
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Author : Dongqing Li
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-08-06
Encyclopedia Of Microfluidics And Nanofluidics written by Dongqing Li and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-08-06 with Technology & Engineering categories.
Covering all aspects of transport phenomena on the nano- and micro-scale, this encyclopedia features over 750 entries in three alphabetically-arranged volumes including the most up-to-date research, insights, and applied techniques across all areas. Coverage includes electrical double-layers, optofluidics, DNC lab-on-a-chip, nanosensors, and more.