Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy

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Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy

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Author : T.G. Dewey
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-06-29

Biophysical And Biochemical Aspects Of Fluorescence Spectroscopy written by T.G. Dewey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-06-29 with Science categories.

Fluorescence spectroscopy has traditionally found wide application in bio chemistry and cell biology. Since there are relatively few naturally occurring fluorescent biomolecules, fluorescence spectroscopy offers a combination of great specificity and sensitivity. Historically, these features have been ex ploited with great success utilizing both intrinsic and extrinsic probes. Re cent applications have built upon these traditional strengths and have re sulted in the development of new instrumental techniques, novel and convenient fluorescent probes, and a deeper, theoretical understanding of fundamental processes. Frequently, fluorescence techniques are tailored to attack a specific biological problem. These new methods in turn produce new physical situations and phenomena which are often of interest to the physical chemist. Thus, progress in one area stimulates renewed interest in other areas. The goal of this book is to provide detailed monographs on the use of fluorescence to investigate problems at the forefront of biochemistry and cell biology. This book is not meant to be a comprehensive survey but rather to highlight areas of recent developments. It is designed to be readable to the novice and yet provide sufficient detail for the expert to keep abreast of recent developments. The book is organized so that it proceeds from simple biochemical sys tems to more complex cell biological ones. Chapter I on fluorescence quenching of biological structures is a good introductory chapter. It intro duces a number of elementary concepts and discusses applications to pro teins and biomembranes.

Principles Of Fluorescence Spectroscopy

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Author : Joseph R. Lakowicz
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-12-05

Principles Of Fluorescence Spectroscopy written by Joseph R. Lakowicz and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-12-05 with Science categories.

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

National Library Of Medicine Current Catalog

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Author : National Library of Medicine (U.S.)
language : en
Publisher:
Release Date : 1993

National Library Of Medicine Current Catalog written by National Library of Medicine (U.S.) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Medicine categories.

Energetics Of Biological Macromolecules Part C

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Author :
language : en
Publisher: Elsevier
Release Date : 2000-08-09

Energetics Of Biological Macromolecules Part C written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-08-09 with Science categories.

Volume 323 of Methods in Enzymology is dedicated to the energetics of biological macromolecules. Understanding the molecular mechanisms underlying a biological process requires detailed knowledge of the structural relationships within the system and an equally detailed understanding of the energetic driving forces that control the structural interactions. This volume presents modern thermodynamic techniques currently being utilized to study the energetic driving forces in biological systems. It will be a useful reference source and textbook for scientists and students whose goal is to understand the energetic relationships between macromoleculer structures and biological functions. This volume supplements Volumes 259 and Volume 295 of Methods in Enzymology.Key Features* Probing Stability of Helical Transmembrane Proteins* Energetics of Vinca Alkaloid Interactions with Tubulin* Deriving Complex Ligand Binding Formulas* Mathematical Modeling of Cooperative Interactions in Hemoglobin* Analysis of Interactions of Regulatory Protein TyrR with DNA* Parsing Free Energy of Drug-DNA Interactions* Use of Fluorescence as Thermodynamics Tool

Green Fluorescent Proteins

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Author : American Society for Cell Biology
language : en
Publisher: Gulf Professional Publishing
Release Date : 1999

Green Fluorescent Proteins written by American Society for Cell Biology and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.

This volume is an authoritative and comprehensive treatment of the approaches and techniques used for Green Fluorescent Proteins (GFP). The primary focus of this work is on research using biological systems. The volume covers all aspects of GFP, from its expression in different organisms to specific microscopic and data analysis methods. Key Features * Only volume on Green Fluorescent Protein research * Covers all aspects of GFP * Provides specific microscopic and data analysis methods * Discusses the design and construction of GFP fusion proteins * Covers GFP expression in animals, insects, plants, and microbes * Details procedures for time lapse imaging of living cells * Explains how to implement single molecule fluorescence detection with GFP * Discusses dual label GFP strategies for multicolor fluorescence * Presents fluorescence resonance energy transfer methods with GFPs * Details quantitative fluorescence imaging techniques * Extensively illustrated with color photographs

Spectrophotometry And Spectrofluorimetry

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Author : Mike Gore
language : en
Publisher: OUP Oxford
Release Date : 2000-05-04

Spectrophotometry And Spectrofluorimetry written by Mike Gore and has been published by OUP Oxford this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-05-04 with Science categories.

Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.

Analytical Instrumentation Handbook Second Edition

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Author : Galen Wood Ewing
language : en
Publisher: CRC Press
Release Date : 1997-08-29

Analytical Instrumentation Handbook Second Edition written by Galen Wood Ewing and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-08-29 with Science categories.

Intended for both the novice and professional, this text aims to approach problems with currently available tools and methods in the modern analytical chemistry domain. It covers all fields from basic theory and principles of analytical chemistry to instrumentation classification, design and purchasing. This edition includes information on X-ray methods and analysis, capillary electrophoresis, infrared and Raman technique comparisons, and more.

Analytical Instrumentation Handbook

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Author : Jack Cazes
language : en
Publisher: CRC Press
Release Date : 2004-11-30

Analytical Instrumentation Handbook written by Jack Cazes and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-11-30 with Science categories.

Compiled by the editor of Dekker's distinguished Chromatographic Science series, this reader-friendly reference is as a unique and stand-alone guide for anyone requiring clear instruction on the most frequently utilized analytical instrumentation techniques. More than just a catalog of commercially available instruments, the chapters are wri

Applied Fluorescence In Chemistry Biology And Medicine

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Author : Wolfgang Rettig
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Applied Fluorescence In Chemistry Biology And Medicine written by Wolfgang Rettig and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The light of the sun - the source of life and a god in ancient times - was always associated with warmth or even burning heat. When man learnt to tame fire, he had another source of light, which was also hot. All the more puzzling for the amazed observer has been the dance of fireflies glimmering in the dark. How can an animal emit light? Or what about the faint glow of walls in some pre historic tombs due to luminescent bacteria? This sort of "cold light" is known today as chemiluminescence. A related phenomenon is the aurora borealis with its swiftly moving curtains oflight. This is a special sort of electroluminescence, of "cold light". another kind The basis of "hot light" is the thermal generation of electronically excited states (normally of atoms or ions). The source of "cold light", on the other hand, is the chemical or electrochemical generation of excited states, possible also for larger molecules. By using light instead of chemical or electrical energy, we can generate yet another type of "cold light", the ordinary luminescence: fluo rescence or phosphorescence. The possibilities here have increased enormously because we can color-tune the exciting light and gain specificity, and we can modulate it in time or even in polarization opening new dimensions of research and applications.

Molecular Fluorescence

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Author : Bernard Valeur
language : en
Publisher: John Wiley & Sons
Release Date : 2013-03-27

Molecular Fluorescence written by Bernard Valeur and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-27 with Science categories.

Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.