Bioreactors For Stem Cell Expansion And Differentiation

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Bioreactors For Stem Cell Expansion And Differentiation

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Author : Joaquim M.S. Cabral
language : en
Publisher: CRC Press
Release Date : 2018-09-03

Bioreactors For Stem Cell Expansion And Differentiation written by Joaquim M.S. Cabral and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-09-03 with Medical categories.

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for tissue engineering, including sophisticated and cost-effective manufacturing strategies geared to overcome technological shortcomings that currently preclude advances towards product commercialization. This book in the fields of stem cell expansion, bioreactors, bioprocessing, and bio and tissue engineering, gives the reader a full understanding of the state-of-art and the future of these fields. Key selling features: Describes various bioreactors or stem cell culturing systems Reviews methods for stem cell expansion and differentiation for neural, cardiac, hemopoietic, mesenchymal, hepatic and other tissues cell types Distinguishes different types of bioreactors intended for different operational scales of tissue engineering and cellular therapies Includes contributions from an international team of leaders in stem cell research

Biopharmaceutical Processing

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Author : Gunter Jagschies
language : en
Publisher: Elsevier
Release Date : 2018-01-18

Biopharmaceutical Processing written by Gunter Jagschies and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-01-18 with Technology & Engineering categories.

Biopharmaceutical Processing: Development, Design, and Implementation of Manufacturing Processes covers bioprocessing from cell line development to bulk drug substances. The methods and strategies described are essential learning for every scientist, engineer or manager in the biopharmaceutical and vaccines industry. The integrity of the bioprocess ultimately determines the quality of the product in the biotherapeutics arena, and this book covers every stage including all technologies related to downstream purification and upstream processing fields. Economic considerations are included throughout, with recommendations for lowering costs and improving efficiencies. Designed for quick reference and easy accessibility of facts, calculations and guidelines, this book is an essential tool for industrial scientists and managers in the biopharmaceutical industry. - Offers a comprehensive, go-to reference for daily work decisions - Covers both upstream and downstream processes - Includes case studies that emphasize financial outcomes - Presents summaries, decision grids, graphs and overviews for quick reference

Bioreactors

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Author : Carl-Fredrik Mandenius
language : en
Publisher: John Wiley & Sons
Release Date : 2016-02-16

Bioreactors written by Carl-Fredrik Mandenius and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-02-16 with Science categories.

In this expert handbook both the topics and contributors are selected so as to provide an authoritative view of possible applications for this new technology. The result is an up-to-date survey of current challenges and opportunities in the design and operation of bioreactors for high-value products in the biomedical and chemical industries. Combining theory and practice, the authors explain such leading-edge technologies as single-use bioreactors, bioreactor simulators, and soft sensor monitoring, and discuss novel applications, such as stem cell production, process development, and multi-product reactors, using case studies from academia as well as from industry. A final section addresses the latest trends, including culture media design and systems biotechnology, which are expected to have an increasing impact on bioreactor design. With its focus on cutting-edge technologies and discussions of future developments, this handbook will remain an invaluable reference for many years to come.

Stem Cell Bioprocessing And Manufacturing

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Author : Joaquim M. S. Cabral
language : en
Publisher: MDPI
Release Date : 2021-01-20

Stem Cell Bioprocessing And Manufacturing written by Joaquim M. S. Cabral and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-01-20 with Science categories.

The next healthcare revolution will apply regenerative medicines using human cells and tissues. The aim of the regenerative medicine approach is to create biological therapies or substitutes in vitro for the replacement or restoration of tissue function in vivo lost through failure or disease. However, whilst science has revealed the potential, and early products have shown the power of such therapies, there is an immediate and long-term need for expertise with the necessary skills to face the engineering and life science challenges before the predicted benefits in human healthcare can be realized. Specifically, there is a need for the development of bioprocess technology for the successful transfer of laboratory-based practice of stem cell and tissue culture to the clinic as therapeutics through the application of engineering principles and practices. This Special Issue of Bioengineering on Stem Cell Bioprocessing and Manufacturing addresses the central role in defining the engineering sciences of cell-based therapies, by bringing together contributions from worldwide experts on stem cell biology and engineering, bioreactor design and bioprocess development, scale-up, and manufacturing of stem cell-based therapies.

Molecular Cellular And Tissue Engineering

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Author : Joseph D. Bronzino
language : en
Publisher: CRC Press
Release Date : 2018-10-08

Molecular Cellular And Tissue Engineering written by Joseph D. Bronzino and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-08 with Medical categories.

Known as the bible of biomedical engineering, The Biomedical Engineering Handbook, Fourth Edition, sets the standard against which all other references of this nature are measured. As such, it has served as a major resource for both skilled professionals and novices to biomedical engineering. Molecular, Cellular, and Tissue Engineering, the fourth volume of the handbook, presents material from respected scientists with diverse backgrounds in molecular biology, transport phenomena, physiological modeling, tissue engineering, stem cells, drug delivery systems, artificial organs, and personalized medicine. More than three dozen specific topics are examined, including DNA vaccines, biomimetic systems, cardiovascular dynamics, biomaterial scaffolds, cell mechanobiology, synthetic biomaterials, pluripotent stem cells, hematopoietic stem cells, mesenchymal stem cells, nanobiomaterials for tissue engineering, biomedical imaging of engineered tissues, gene therapy, noninvasive targeted protein and peptide drug delivery, cardiac valve prostheses, blood substitutes, artificial skin, molecular diagnostics in personalized medicine, and bioethics.

Encyclopedia Of Tissue Engineering And Regenerative Medicine

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Author :
language : en
Publisher: Academic Press
Release Date : 2019-06-03

Encyclopedia Of Tissue Engineering And Regenerative Medicine written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-06-03 with Science categories.

Encyclopedia of Tissue Engineering and Regenerative Medicine, Three Volume Set provides a comprehensive collection of personal overviews on the latest developments and likely future directions in the field. By providing concise expositions on a broad range of topics, this encyclopedia is an excellent resource. Tissue engineering and regenerative medicine are relatively new fields still in their early stages of development, yet they already show great promise. This encyclopedia brings together foundational content and hot topics in both disciplines into a comprehensive resource, allowing deeper interdisciplinary research and conclusions to be drawn from two increasingly connected areas of biomedicine. Provides a ‘one-stop’ resource for access to information written by world-leading scholars in the fields of tissue engineering and regenerative medicine Contains multimedia features, including hyperlinked references and further readings, cross-references and diagrams/images Represents the most comprehensive and exhaustive product on the market on the topic

Cell And Tissue Reaction Engineering

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Author : Regine Eibl
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-09-30

Cell And Tissue Reaction Engineering written by Regine Eibl and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-09-30 with Science categories.

The completion of the Human Genome Project and the rapid progress in cell bi- ogy and biochemical engineering, are major forces driving the steady increase of approved biotech products, especially biopharmaceuticals, in the market. Today mammalian cell products (“products from cells”), primarily monoclonals, cytokines, recombinant glycoproteins, and, increasingly, vaccines, dominate the biopharmaceutical industry. Moreover, a small number of products consisting of in vitro cultivated cells (“cells as product”) for regenerative medicine have also been introduced in the market. Their efficient production requires comprehensive knowledge of biological as well as biochemical mammalian cell culture fundamentals (e.g., cell characteristics and metabolism, cell line establishment, culture medium optimization) and related engineering principles (e.g., bioreactor design, process scale-up and optimization). In addition, new developments focusing on cell line development, animal-free c- ture media, disposables and the implications of changing processes (multi-purpo- facilities) have to be taken into account. While a number of excellent books treating the basic methods and applications of mammalian cell culture technology have been published, only little attention has been afforded to their engineering aspects. The aim of this book is to make a contribution to closing this gap; it particularly focuses on the interactions between biological and biochemical and engineering principles in processes derived from cell cultures. It is not intended to give a c- prehensive overview of the literature. This has been done extensively elsewhere.

The Biomedical Engineering Handbook

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Author : Joseph D. Bronzino
language : en
Publisher: CRC Press
Release Date : 2018-10-03

The Biomedical Engineering Handbook written by Joseph D. Bronzino and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-03 with Medical categories.

The definitive bible for the field of biomedical engineering, this collection of volumes is a major reference for all practicing biomedical engineers and students. Now in its fourth edition, this work presents a substantial revision, with all sections updated to offer the latest research findings. New sections address drugs and devices, personalized medicine, and stem cell engineering. Also included is a historical overview as well as a special section on medical ethics. This set provides complete coverage of biomedical engineering fundamentals, medical devices and systems, computer applications in medicine, and molecular engineering.

Bioprocess Engineering For A Green Environment

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Author : V. Sivasubramanian
language : en
Publisher: CRC Press
Release Date : 2018-05-04

Bioprocess Engineering For A Green Environment written by V. Sivasubramanian and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-04 with Medical categories.

Bioprocess Engineering for a Green Environment examines numerous bioprocesses that are crucial to our day-to-day life, specifically the major issues surrounding the production of energy relating to biofuels and waste management. The nuance of this discussion is reflected by the text’s chapter breakdown, providing the reader with a fulsome investigation of the energy sector; the importance of third-generation fuels; and the application of micro- and macroalgae for the production of biofuels. The book also provides a detailed exploration of biocatalysts and their application to the food industry; bioplastics production; conversion of agrowaste into polysaccharides; as well as the importance of biotechnology in bio-processing. Numerous industries discharge massive amounts of effluents into our rivers, seas, and air systems. As such, two chapters are dedicated to the treatment of various pollutants through biological operation with hopes of achieving a cleaner, greener, environment. This book represents the most comprehensive study of bioprocessing—and its various applications to the environment—available on the market today. It was furthermore written with various researchers in mind, ranging from undergraduate and graduate students looking to enhance their knowledge of the topics presented to scholars and engineers interested in the bioprocessing field, as well as members of industry and policy-makers. Provides a comprehensive overview of bioprocesses that apply to day-to-day living. Is learner-centered, providing detailed diagrams for easy understanding. Explores the importance of biocatalysts and their applications to the food industry, as well as bioplastics production. Examines the unique capabilities of bioprocess engineering and its ability to treat various pollutants. .

Development Of Miniaturized Bioreactors For Stem Cell Culture

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Author : Mariam Sarvi
language : en
Publisher:
Release Date : 2014

Development Of Miniaturized Bioreactors For Stem Cell Culture written by Mariam Sarvi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with categories.

Embryonic stem cells (ESCs) are pluripotent cells capable of indefinite self renewal in vitro while maintaining the ability to differentiate into cell types of all three germ layers. ESCs are outstanding options of in vitro cell models for regenerative medicine, functional genomics, human developmental biology and drug discovery study. Stem cell research is among the most promising fields of biotechnology, and which provides the potential of developing novel approaches to repair or replace damaged tissues and cells. The present day, exponentially growing effort of stem cell research emphasizes a major need for convergence of more efficient and appropriate laboratory technologies to sustain the growth, proliferation and differentiation potential of stem cells.Although so far, a variety of conventional bioreactors with different configurations (such as spinner flasks, rotary, perfusion bioreactor, etc.) have been designed and adapted for stem cell expansion and differentiation, bioreactors can be disadvantageous in bench-top research because they need large space, consume huge amount of reagents and need more time to operate and maintain (sterilizing, cleaning, assembling, and disassembling of the bioreactor components). The requirements for costly equipment and generating shear stress due to the fluid flow, and the lack of physical similarity between microenvironments of bioreactor and actual cell microenvironment, make using bioreactors undesirable.In tissue engineering, micro-scale technology is an approach that combines micro-techniques with materials science and surface engineering, and results in a profound exploration of the microenvironment where cells are embedded. These technologies are able to address some of the limitations imposed by conventional tissue engineering methods. Indeed, developing successful novel small-scale technologies for in vitro cultivation of different types of cells can assist in increasing our knowledge on conditions that control stem cell growth and differentiation and organ development. In fact, small scale bioreactors are miniaturized versions of conventional bioreactors, where high- throughput cell based assays can be carried out at low cost compared with their macro-scale counter- parts.The first aim of this thesis was to develop a disposable miniaturized bioreactor through a novel and inexpensive method for effective stem cell proliferation. To this end, an effective surface functionalization method was developed for enhancing the biocompatibility of a PDMS surface that is protein resistant while facilitating cell proliferation (expansion) and maintaining the pluripotency potential of cells. The micro-bioreactor was fabricated in the form of a fixed bed bioreactor with a microchannel reactor bed. The microchannel was functionalized to enable cell adhesion and resistance to protein adsorption. The functionalized surface was found to be biocompatible with cancer and embryonic stem cells (ESCs), and while facilitating cell proliferation.Differentiation of ESCs into a variety of cell types is an important characteristic of these types of cells, which is commonly achieved in vitro by spontaneously self-assembling in low adhesion culture conditions into 3D cell aggregates called embryoid bodies (EBs). Formation of EBs that simulates many of the characteristics of early embryonic development is considered as a vital step to induce differentiation of stem cells. Formation of three dimensional configurations of ESCs as EBs provides possibilities to mechanistically study early differentiation events of pluripotent cells. In fact, EB formation is of paramount importance for in vitro investigation of the embryonic development and differentiation of both the mouse and human ES cells.The second aim of this thesis was to introduce a novel concept of a miniaturized bioreactor made of liquid marble (LM). A novel application of liquid marbles for formation of embryoid bodies (EBs) was then presented. This study showed that the confined internal space of liquid marble, along with the porous and non-adhesive property of the highly hydrophobic liquid marble shell, can facilitate the formation of uniform EBs inside the liquid marbles. The efficiency of liquid marble-born-EBs compared to the liquid suspension (LS) technique as the chosen control method in terms of cell viability and EB uniformity revealed that cells in liquid marble are more viable than those in suspension. Measuring EB size distribution as an indicator of uniformity also confirmed that EBs obtained by the LM are morphologically more uniform and of a narrower size distribution compared to those formed in LS. The feasibility of using liquid marble bioreactors for cardiomyocyte differentiation of mouse ES (mES) cells after formation of EBs inside the liquid marble was further investigated. The results demonstrated that ES cells can differentiate into myocyte cells through the liquid marble as a facile, cost effective, and straightforward method. We proposed for the first time that liquid marbles greatly contribute to ES cardiac differentiation, which provides a new technology platform for ES biology and genetic studies.It is worth mentioning that although the majority of our knowledge in modern biology has been provided by classical two dimensional (2D) cell culture techniques, the most common negative aspects of these systems is deficiency of support from extra-cellular matrix, which represents an important role for cell growth and development. It is now well accepted that cells reside, proliferate, and differentiate in complex 3D microenvironments. The concept of using three dimensional (3D) biodegradable scaffolds as alternatives for extracellular matrix (ECM), which more closely reform cells' native structure, is an interesting area of study in current tissue engineering. Because of their unique function, stem cells need to reside in the specialized, 3D microenvironment that surrounds them in native tissues.The third section of this thesis (chapter 5) focuses on the investigating of the feasibility of forming embryoid bodies using a novel hydrogel as bioreactor embedding material. This hydrogel is porous and biodegradable and is prepared by modifying hydroxypropylcellulose (HPC), with bio-functional methacrylates (MA). Observation of EB formation inside hydrogel implied that the stem cells attached and penetrated to the pores, and proliferated well, while forming uniform EBs. Uniformity of EBs formed inside hydrogel, compared with those formed via liquid suspension (LS) method, as one of the most widely used EB formation techniques. It was observed that porous hydrogel allows the formation of more homogeneous EBs. It was found that cells inside hydrogel-born EBs are more viable compared to those formed in LS method. Expression of germ markers via quantitative PCR and immunostaining confirmed that the hydrogel-born EBs had expressed 3 germ layers with further in vitro differentiation potential. These EBs were allowed to differentiate further in hydrogel, where positive immunostaining of different cardiac markers and observation of beating EBs showed the potential of EBs to further differentiate into cardiac cells lineage.In summary, this thesis first presents a novel, facile and cost effective method via surface bio- functionalization of PDMS bioreactor for better stem cell adhesion and proliferation, and later introduces two novel methods to prepare bioreactor material, namely liquid marble and porous hydrogel (HPC-MA) for formation of embryoid bodies, which is considered as a critical step for in vitro differentiation in ESCs.