Calcium Signaling Protocols
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Calcium Signaling Protocols
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Author : David G. Lambert
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Calcium Signaling Protocols written by David G. Lambert and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
In the first edition of Calcium Signaling Protocols I began by writing “The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. ” This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.
Methods In Molecular Biology Calcium Signaling Protocols
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Author : John M. Walker
language : en
Publisher:
Release Date : 1984
Methods In Molecular Biology Calcium Signaling Protocols written by John M. Walker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1984 with Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols categories.
Calcium Signalling
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Author : Anna Raffaello
language : en
Publisher:
Release Date : 2019
Calcium Signalling written by Anna Raffaello and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with Biochemistry categories.
This volume looks at the latest developments in studying calcium signaling. The chapters in this book discuss topics such as in vitro and in vivo analyses in model systems; methods to measure Ca2+ in different subcellular compartments; single Ca2+ channels; methods to measure cellular ROS and ATP; and the functionality of the ATP synthase. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Calcium Signalling: Methods and Protocols is a valuable resource that covers both conceptual and methodological viewpoints to aid beginners and experts in furthering their studies in the developing field of calcium homeostasis research.
Calcium Signaling
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Author : Caroline M. Gorvin
language : en
Publisher: Springer Nature
Release Date : 2024-10-12
Calcium Signaling written by Caroline M. Gorvin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-12 with Science categories.
This detailed volume explores techniques for performing calcium signaling in diverse cell types, alongside detailed methods for data analysis. Beginning with chapters on cellular assays to assess calcium signaling, the book continues with sections on imaging methods, methods to assess calcium within primary cilia, the endoplasmic reticulum, and the mitochondria, as well as tissue-specific calcium signaling methods, including the establishment of primary cell or tissue models and measuring calcium in whole organisms such as Drosophila. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Calcium Signaling: Methods and Protocols serves as an ideal guide for laboratories of all types seeking to measure calcium robustly.
Flavoprotein Protocols
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Author : Steven K. Chapman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flavoprotein Protocols written by Steven K. Chapman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Calcium Signaling
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Author : James W. Putney Jr.
language : en
Publisher: CRC Press
Release Date : 2005-11-21
Calcium Signaling written by James W. Putney Jr. and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-11-21 with Medical categories.
The first edition of James Putney's Calcium Signaling offered readers a comprehensive view of the fascinating diversity of technologies that the new field of calcium signaling employed. And while that work is still regarded as a premier text on the basics of calcium signaling, progress has been so dramatic that an update is now required. In Cal
Human Retrovirus Protocols
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Author : Tuofu Zhu
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Human Retrovirus Protocols written by Tuofu Zhu and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A cutting-edge collection of basic and state-of-the-art methods optimized for investigating the molecular biology of this class of retrovirus. These readily reproducible techniques range from methods for the isolation and detection of human retroviruses to cutting-edge methods for exploring the interplay between the viruses and the host. Here, the researcher will find up-to-date techniques for the isolation and propagation of HIV, HTLV, and foamy virus from a variety of sources. There are also assays for determining the cell tropism of HIV-1, the coreceptor usage of HIV-1, and human gene expression with HIV-1 infection by microarrays, as well as for phenotyping HIV-1 infected monocytes and examining their fitness. Highlights include the detection and quantification of HIV-1 in resting CD4+, a new cloning system for making recombinent virus, cDNA microarrays, and the determination of genetic polymorphisms in two recently identified HIV-1 co-factors that are critical for HIV-1 infection.
Receptor Binding Techniques
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Author : Mary Keen
language : en
Publisher: Springer Science & Business Media
Release Date : 1999
Receptor Binding Techniques written by Mary Keen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Medical categories.
This cutting-edge collection of step-by-step experimental protocols demonstrates
Cell Imaging Techniques
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Author : Douglas J. Taatjes
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Cell Imaging Techniques written by Douglas J. Taatjes and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Receptor Binding Techniques
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Author : Anthony P. Davenport
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Receptor Binding Techniques written by Anthony P. Davenport and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.