Receptor Binding Techniques
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Receptor Binding Techniques
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Author : Anthony P. Davenport
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Receptor Binding Techniques written by Anthony P. Davenport and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
A comprehensive collection of readily reproducible methods for studying receptors in silico, in vitro, and in vivo. These cutting-edge techniques cover mining from curated databases, identifying novel receptors by high throughput screening, molecular methods to identify mRNA encoding receptors, radioligand binding assays and their analysis, quantitative autoradiography, and imaging receptors by positron emission tomography (PET). Highlights include phenotypic characterization of receptors in knockout mice, imaging receptors using green fluorescent protein and fluorescent resonance energy transfer, and quantitative analysis of receptor mRNA by TaqMan PCR. These book equips the researcher with techniques for exploring the unprecedented number of new receptor systems now emerging and the so-called "orphan" receptors whose activating ligand has not been identified.
Principles And Methods In Receptor Binding
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Author : F. Cattabeni
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Principles And Methods In Receptor Binding written by F. Cattabeni and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Medical categories.
One of the major advances in the understanding of the mechanism of action of hormones, neurotransmitters and drugs had arisen from the hypothesis that the physiological or pharmacological responses are trig gered by their interaction with specific cell compo nents, termed receptors. However, the presence of receptors has been inferred from indirect data, and only recently has it been possible to study the kinetics of the interaction between drug and receptors directly, through the so called "binding technique. " This NATO-ASI on "Principles and Methods in Receptor Binding" was devoted mainly to the following aspects of the study of receptors: the principles underlying the use of the binding technique; the mathematical models necessary to interpret binding data; the application of binding methods to specific receptors; and, finally, a few selected examples of coupling between receptors and physiological re sponses. In the chapters of this book, special interest is devoted to the analysis of the simplest models for the interaction of receptors with their ligands (either hormones or neurotransmitters or drugs). The graphical techniques used to analyze the data from binding exper iments are extensively discussed, together with the statistics that have to be used in binding analysis. Moreover, the basic concepts to analyze binding data using a personal computer are presented. v vi PREFACE The factors which should be considered when setting up a binding assay (such as choice of ligand and incubation conditions, preparation of tissue, termina tion of incubation) are discussed.
Receptor Binding In Drug Research
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Author : Robert A. O'Brien
language : en
Publisher: CRC Press
Release Date : 2020-08-26
Receptor Binding In Drug Research written by Robert A. O'Brien and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-08-26 with Science categories.
This book describes the applications of receptor techniques in many different areas in addition to conventional drug and neurotransmitter binding sites. It reviews humoral modulators such as a leukotrienes, interferon, platelet-derived growth factor, and novel endogenous ligands.
Principles And Methods In Receptor Binding
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Author : Flaminio Cattabeni
language : en
Publisher:
Release Date : 2014-01-15
Principles And Methods In Receptor Binding written by Flaminio Cattabeni and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Receptor Binding Techniques
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Author : Mary Keen
language : en
Publisher: Springer Science & Business Media
Release Date : 1999
Receptor Binding Techniques written by Mary Keen and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999 with Binding Sites categories.
This cutting-edge collection of step-by-step experimental protocols demonstrates
Receptor Binding
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Author : Alan A. Boulton
language : en
Publisher: Humana
Release Date : 2013-08-22
Receptor Binding written by Alan A. Boulton and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-08-22 with Medical categories.
Techniques in the neurosciences are evolving rapidly. There are currently very few volumes dedicated to the methodology - ployed by neuroscrentists, and those that are available often seem either out of date or limited in scope. This series is about the methods most widely used by modern-day neuroscientists and 1s written by their colleagues who are practicing experts. Volume 1 will be useful to all neuroscientists since it concerns those procedures used routinely across the widest range of s- drsciplines. Collecting these general techniques together in a single volume stnkes us not only as a service, but will no doubt prove of exceptional utilitarian value as well. Volumes 2 and 3 describe all current procedures for the analyses of ammes and theirmetabolites and of amino acrds, respectively. These collections will clearly be of value to all neuroscientists working in or contemplating research in these fields. Similar reasons exist for Volume 4 on receptor binding techniques since experimental details are provided for many types of ligand-receptor binding, including chapters on general prin- ples, drug discovery and development, and a most useful app- dix on computer programs for Scatchard, nonlinear, and compe- tive displacement analyses. Volume 5 provides procedures for the assessment of enzymes involved in biogenic amine synthesis and catabolism. Volumes in the NEUROMETHODS series will be useful to neurochemists, -pharmacologists, -physrologists, -anatomists, psychopharmacologists, psychiatrists, neurologists, and chemists (organic, analytical, pharmaceutical, medicinal); in fact, everyone involved in the neurosciences, both basic and clinical.
Cell Surface Receptors A Short Course On Theory And Methods
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Author : Lee E. Limbird
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Cell Surface Receptors A Short Course On Theory And Methods written by Lee E. Limbird and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Cell Surface Receptors: A Short Course on Theory and Methods, Second Edition is a primer for the study of cell surface receptors. The simplified discussion of methods and their underlying principles removes the usual intimidation caused by the specialized vocabulary or sophisticated mathematics that characterize many of the primary papers in this field. In this way, the basic concepts become emphasized. This volume is a starting point: a textbook as well as a manual to which the investigator can return for a refresher course, when needed.
Brain Receptor Methodologies Pt A
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Author : Paul J. Marangos
language : en
Publisher: Elsevier
Release Date : 2012-12-02
Brain Receptor Methodologies Pt A written by Paul J. Marangos and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-02 with Science categories.
Brain Receptor Methodologies, Part A, General Methods and Concepts: Amines and Acetylcholine provides information pertinent to neurotransmitter and neuromodulator receptors in brain. This book explores the methodologies that can used to address several basic and clinical problems. Organized into two sections encompassing 18 chapters, this book starts with an overview of the receptor concept, which can be validated from indirect evidence obtained in studies of the quantitative aspects of drug antagonism. This text then examines the radioligand–receptor binding interactions. Other chapters consider immunocytochemistry, which has a primary role in determining the precise distribution of regulatory peptides to neural and endocrine elements of the diffuse neuroendocrine system. The final chapter discusses the use of the radioligand binding procedure for the study of muscarinic receptors, which has expanded the area of muscarinic receptor pharmacology. Biochemists, pharmacologists, physiologists, and researchers engaged in the fields of neurobiology and neuroscience will find this book extremely useful.
Current Directions In Radiopharmaceutical Research And Development
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Author : Steven J. Mather
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Current Directions In Radiopharmaceutical Research And Development written by Steven J. Mather and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
Radiophannaceutical research has recently undergone a major change in direction. In past years it has been concerned mainly with the development of perfusion tracers, the biodistribution of which reflect the regional blood flow to areas of major organs such as the heart and brain. However, a major new direction of interest now lies in the development of receptor-binding radio-tracers which can be used to perform in-vivo characterisation of diseased tissues and it is likely that much of the future research in this field will follow this direction. The difficulties in developing such tracers are considerable. The researcher must first identify a promising target for radiopharmaceutical development. High specific activity radioactive molecules must be designed and synthesised which will both bind to the target receptor with high affinity, and also have the physicochemical characteristics which will allow them to reach the target site in sufficient quantity while at the same time showing minimal uptake in non-target tissues. Thus the knowledge base required for radiophannaceutical development has now expanded beyond the limits of radiopharmaceutical chemistry to include aspects of biochemistry, molecular biology and conventional drug design. The portfolio of basic knowledge required to support current radiopharmaceutical development is changing and scientists working in this arena need to be trained in this regard. At the same time, the very latest developments in the field need to be communicated to the scientific community in order to stimulate the advancement of this exciting new direction of research.
Receptor Localization
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Author : Marjorie Ariano
language : en
Publisher: John Wiley & Sons
Release Date : 1998-07-16
Receptor Localization written by Marjorie Ariano and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-07-16 with Medical categories.
The detection of neurotransmitter receptor locations and distribution densities within the central nervous system and peripheral tissues is receiving intense attention within the neuroscience research community. Neurotransmitter receptors, which receive the chemical signals sent from one neuron to another, are critical links in a highly complex information-processing chain. Pinpointing receptor sites and systems is crucial for understanding neurological function as well as dysfunction. It is also essential for understanding how receptors process information when impacted by such substances as heroin or nicotine, or when affected by neurodegenerative disease. Receptor Localization: Laboratory Methods and Procedures is the first user-friendly guide to the latest techniques and approaches being employed to examine the localization of neurotransmitter receptors in the central nervous system and peripheral tissues. It covers detection methods that are applicable to a wide variety of receptor systems, ranging from genes and ligands to in vito receptors in individuals; and to numerous receptor subtypes, such as nicotine, muscarine, tackykinins, dopamine, adenosine, and GABA. The standard laboratory "recipes" or "tricks" employed in these detection methods are fully discussed, as are the advantages and limitations of each procedure. With contributions from leading experts and extensively illustrated, this book: * Discusses receptor ligand binding methods using irreversible and reversible compounds * Presents antireceptor antisera technology using synthetic peptides and fusion proteins at both the cellular and subcellular resolution levels * Examines molecular assessments of receptors * Describes in situ hybridization, reverse transcriptase-PCR, and fluorescent in situ transcription * Covers new visualization paradigms * Includes physiological analysis of receptor function, cellular detection in the brain slice, and cultured neurons * Discusses the use of PET and SPECT to assess in vivo receptor distributions in animals and humans Receptor Localization: Laboratory Methods and Procedures is an invaluable guide for researchers in the related fields of neurology, biochemistry, and pharmacology. Its lucid descriptions of new detection methods, inclusion of experimental examples, and emphasis on how these experimental approaches are applicable to particular research areas will appeal to both the experienced researcher and novice investigator.