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Capturing Chromosome Conformation


Capturing Chromosome Conformation
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Capturing Chromosome Conformation


Capturing Chromosome Conformation
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Author : Beatrice Bodega
language : en
Publisher:
Release Date : 2021

Capturing Chromosome Conformation written by Beatrice Bodega and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021 with Biology categories.




Capturing Chromosome Conformation


Capturing Chromosome Conformation
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Author : Beatrice Bodega
language : en
Publisher: Humana
Release Date : 2020-10-29

Capturing Chromosome Conformation written by Beatrice Bodega and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-29 with Science categories.


This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.



Chromatin Immunoprecipitation Assays


Chromatin Immunoprecipitation Assays
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Author : Philippe Collas
language : en
Publisher: Humana Press
Release Date : 2012-03-05

Chromatin Immunoprecipitation Assays written by Philippe Collas and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-05 with Science categories.


Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.



Plant Chromatin Dynamics


Plant Chromatin Dynamics
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Author : Célia Baroux
language : en
Publisher:
Release Date : 2018

Plant Chromatin Dynamics written by Célia Baroux and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018 with Botany categories.




Hi C Data Analysis


Hi C Data Analysis
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Author : Silvio Bicciato
language : en
Publisher: Humana
Release Date : 2022-09-04

Hi C Data Analysis written by Silvio Bicciato and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-09-04 with Science categories.


This volume details a comprehensive set of methods and tools for Hi-C data processing, analysis, and interpretation. Chapters cover applications of Hi-C to address a variety of biological problems, with a specific focus on state-of-the-art computational procedures adopted for the data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Hi-C Data Analysis: Methods and Protocols aims to help computational and molecular biologists working in the field of chromatin 3D architecture and transcription regulation.



Research In Computational Molecular Biology


Research In Computational Molecular Biology
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Author : Minghua Deng
language : en
Publisher: Springer
Release Date : 2013-03-22

Research In Computational Molecular Biology written by Minghua Deng and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-22 with Computers categories.


This book constitutes the refereed proceedings of the 17th Annual International Conference on Research in Computational Molecular Biology, RECOMB 2013, held in Beijing, China, in April 2013. The 32 revised full papers were carefully reviewed and selected from 167 submissions. The papers cover a wide range of topics including molecular sequence analysis; genes and regulatory elements; molecular evolution; gene expression; biological networks; sequencing and genotyping technologies; genomics; epigenomics; metagenomics; population, statistical genetics; systems biology; computational proteomics; computational structural biology; imaging; large-scale data management.



Eukaryotic Transcriptional And Post Transcriptional Gene Expression Regulation


Eukaryotic Transcriptional And Post Transcriptional Gene Expression Regulation
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Author : Narendra Wajapeyee
language : en
Publisher:
Release Date : 2016

Eukaryotic Transcriptional And Post Transcriptional Gene Expression Regulation written by Narendra Wajapeyee and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016 with Biomedicine categories.


This volume describes a variety of protocols that will allow the readers to study different aspects of transcriptional and posttranscriptional gene expression regulation in eukaryotic cells. Chapters focus on the latest use of CRISPRi and RNAi technologies for studying various aspects of transcriptional and posttranscriptional regulation and tools to navigate protocols on key bioinformatics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Eukaryotic Transcription and Post-Transcription Gene Expression Regulation aims to ensure successful results in the further study of this vital field.



Innate Antiviral Immunity


Innate Antiviral Immunity
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Author : Karen Mossman
language : en
Publisher:
Release Date : 2017

Innate Antiviral Immunity written by Karen Mossman and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017 with Biomedicine categories.


This book explores methods to study the complex and evolving interplay between a virus and its host that range from model systems to the detection of chemical molecules. The collection starts with the application of humanized mice and zebrafish as model organisms to study virus-host interactions and induction of innate immune responses. Subsequent chapters outline diverse methods to detect small interfering RNAs, microRNAs, and virus-derived dsRNA from a variety of cells, tissues, and organisms, as well as to interrogating the cytosolic RNA and DNA sensing pathways, including using RNA PAMPs as molecular tools, purification of cGAMP from virus particles and infected cells, and mechanisms to visualize the subcellular localization and activation of the adaptor proteins MAVS and STING. Cutting-edge methods, including high-throughput and genome-wide CRISPR/Cas9 screens, chromosome conformation capture, and whole-exome sequencing, are described to identify novel mediators, pathways, and variants underlying host susceptibility. Given the importance of studying these pathways and players under physiologic conditions, methods describing the isolation of primary mouse sensory neurons and group 2 innate lymphoid cells are also provided. Finally, this collection comes full circle back to the whole organism level and concludes with epidemiological methods to investigate virus-host interactions and the induction of innate immunity. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Innate Antiviral Immunity: Methods and Protocols spans a diverse array of approaches to study and elucidate the intricacies of this vital area of study.



Hic Pro An Optimized And Flexible Pipeline For Hi C Data Processing


Hic Pro An Optimized And Flexible Pipeline For Hi C Data Processing
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Author : Oldenburg Oldenburg Press
language : en
Publisher:
Release Date : 2016-01-29

Hic Pro An Optimized And Flexible Pipeline For Hi C Data Processing written by Oldenburg Oldenburg Press and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-29 with categories.


HiC-Pro is an optimized and flexible pipeline for processing Hi-C data from raw reads to normalized contact maps. HiC-Pro maps reads, detects valid ligation products, performs quality controls and generates intra- and inter-chromosomal contact maps. It includes a fast implementation of the iterative correction method and is based on a memory-efficient data format for Hi-C contact maps. In addition, HiC-Pro can use phased genotype data to build allele-specific contact maps. We applied HiC-Pro to different Hi-C datasets, demonstrating its ability to easily process large data in a reasonable time. Source code and documentation are available at http://github.com/nservant/HiC-Pro.



Plant Epigenetics Methods And Protocols


Plant Epigenetics Methods And Protocols
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Author : Igor Kovalchuk
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-11-05

Plant Epigenetics Methods And Protocols written by Igor Kovalchuk and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-11-05 with Science categories.


This volume provides a variety of protocols to analyze various epigenetic changes, including differential expression of non-coding RNAs, changes in DNA methylation, and histone modifications in plants. Chapters detail protocols with different degrees of complexity, and describe bioinformatics approaches for data processing and analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.