Cell Analysis
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Computational Methods For Single Cell Data Analysis
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Author : Guo-Cheng Yuan
language : en
Publisher: Humana Press
Release Date : 2019-02-14
Computational Methods For Single Cell Data Analysis written by Guo-Cheng Yuan and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-14 with Science categories.
This detailed book provides state-of-art computational approaches to further explore the exciting opportunities presented by single-cell technologies. Chapters each detail a computational toolbox aimed to overcome a specific challenge in single-cell analysis, such as data normalization, rare cell-type identification, and spatial transcriptomics analysis, all with a focus on hands-on implementation of computational methods for analyzing experimental data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computational Methods for Single-Cell Data Analysis aims to cover a wide range of tasks and serves as a vital handbook for single-cell data analysis.
Microfluidics For Single Cell Analysis
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Author : Jin-Ming Lin
language : en
Publisher: Springer Nature
Release Date : 2019-08-28
Microfluidics For Single Cell Analysis written by Jin-Ming Lin and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-08-28 with Science categories.
This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Single Cell Analysis
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Author : Dario Anselmetti
language : en
Publisher: John Wiley & Sons
Release Date : 2009-03-09
Single Cell Analysis written by Dario Anselmetti and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-03-09 with Science categories.
The first-ever comprehensive overview of the methods used in this key technology in modern biology provides the latest working knowledge needed by every scientist entering this growing field. It covers all the current technology and application areas, from microscopy and spectroscopy to proteomics and microfluidics.
Cell Analysis On Microfluidics
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Author : Jin-Ming Lin
language : en
Publisher: Springer
Release Date : 2017-10-25
Cell Analysis On Microfluidics written by Jin-Ming Lin and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-10-25 with Science categories.
This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Essentials Of Single Cell Analysis
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Author : Fan-Gang Tseng
language : en
Publisher: Springer
Release Date : 2016-01-21
Essentials Of Single Cell Analysis written by Fan-Gang Tseng and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-01-21 with Technology & Engineering categories.
This book provides an overview of single-cell isolation, separation, injection, lysis and dynamics analysis as well as a study of their heterogeneity using different miniaturized devices. As an important part of single-cell analysis, different techniques including electroporation, microinjection, optical trapping, optoporation, rapid electrokinetic patterning and optoelectronic tweezers are described in detail. It presents different fluidic systems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry) and their integration with sensor technology, optical and hydrodynamic stretchers etc., and demonstrates the applications of single-cell analysis in systems biology, proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics, biomedicine and drug delivery systems. It also discusses the future challenges for single-cell analysis, including the advantages and limitations. This book is enjoyable reading material while at the same time providing essential information to scientists in academia and professionals in industry working on different aspects of single-cell analysis. Dr. Fan-Gang Tseng is a Distinguished Professor of Engineering and System Science at the National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.
Single Cell Analysis In Biotechnology And Systems Biology
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Author : Fan-Gang Tseng
language : en
Publisher: MDPI
Release Date : 2018-10-01
Single Cell Analysis In Biotechnology And Systems Biology written by Fan-Gang Tseng and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-01 with Science categories.
This book is a printed edition of the Special Issue "Single Cell Analysis in Biotechnology and Systems Biology" that was published in IJMS
Micro Nanofluidic Devices For Single Cell Analysis
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Author : Fan-Gang Tseng
language : en
Publisher: MDPI
Release Date : 2018-10-04
Micro Nanofluidic Devices For Single Cell Analysis written by Fan-Gang Tseng and has been published by MDPI this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-10-04 with Computers categories.
This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines
The Individual Microbe Single Cell Analysis And Agent Based Modelling
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Author : Johan H. J. Leveau
language : en
Publisher: Frontiers Media SA
Release Date : 2019-02-19
The Individual Microbe Single Cell Analysis And Agent Based Modelling written by Johan H. J. Leveau and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-19 with categories.
Recent technological advances in single-cell microbiology, using flow cytometry, microfluidics, x-ray fluorescence microprobes, and single-cell -omics, allow for the observation of individuals within populations. Simultaneously, individual-based models (or more generally agent-based models) allow for individual microbes to be simulated. Bridging these techniques forms the foundation of individual-based ecology of microbes (µIBE). µIBE has elucidated genetic and phenotypic heterogeneity that has important consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. Individual-based models can help us to understand how these sets of traits of individual microbes influence the above. This eBook compiles all publications from a recent Research Topic in Frontiers in Microbiology. It features recent research where individual observational and/or modelling techniques are applied to gain unique insights into the ecology of microorganisms. The Research Topic “The Individual Microbe: Single-Cell Analysis and Agent-Based Modelling” arose from the 2016 @ASM conference of the same name hosted by the American Society for Microbiology at its headquarters in Washington, D.C. We are grateful to ASM for funding and hosting this conference.
Biosensors For Single Cell Analysis
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Author : Jian Chen
language : en
Publisher: Academic Press
Release Date : 2021-10-26
Biosensors For Single Cell Analysis written by Jian Chen and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-26 with Science categories.
Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers. - Includes chapters focused on key single-cell properties, such as proteomic, metabolic and mechanical characterization - Features case studies that illustrate the application of biosensors for single-cell analysis - Considers microfluidic approaches for each single-cell property discussed - Explores future directions for single-cell analysis and biosensor technology
Emerging Tools For Single Cell Analysis
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Author : Gary Durack
language : en
Publisher: John Wiley & Sons
Release Date : 2004-03-24
Emerging Tools For Single Cell Analysis written by Gary Durack and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-03-24 with Science categories.
The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies. Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis. By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,