Cellular Imaging
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Methods In Cellular Imaging
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Author : Ammasi Periasamy
language : en
Publisher: Springer
Release Date : 2013-05-27
Methods In Cellular Imaging written by Ammasi Periasamy and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-27 with Science categories.
Advances in technology have revolutionized the development of light microscopy techniques in biomedical research, thus improving visualization of the microstructure of cells and tissues under physiological conditions. Fluorescence microscopy methods are non-contact and non-invasive and provide high spatial and temporal resolution that other laboratory techniques cannot. This well-illustrated book targets graduate students and scientists who are new to the state-of-the-art fluorescence microscopy techniques used in biological and clinical imaging. It explains basic concepts and imaging procedures for wide-field, confocal, multiphoton excitation, fluorescence resonance energy transfer (FRET), lifetime imaging (FLIM), spectral imaging, fluorescence recovery after photobleaching (FRAP), optical tweezers, total internal reflection, high spatial resolution atomic force microscopy (AFM), and bioluminescence imaging for gene expression. The usage of these techniques in various biological applications, including calcium, pH, membrane potential, mitochondrial signaling, protein-protein interactions under various physiological conditions, and deep tissue imaging, is clearly presented. The authors describe the approaches to selecting epifluorescence microscopy, the detectors, and the image acquisition and processing software for different biological applications. Step-by-step directions on preparing different digital formats for light microscopy images on websites are also provided.
Cellular Imaging Techniques For Neuroscience And Beyond
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Author : Floris G. Wouterlood
language : en
Publisher: Academic Press
Release Date : 2012-12-06
Cellular Imaging Techniques For Neuroscience And Beyond written by Floris G. Wouterlood and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques
Cellular Imaging
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Author : Eric Hanssen
language : en
Publisher: Springer
Release Date : 2017-12-30
Cellular Imaging written by Eric Hanssen and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-30 with Science categories.
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
Molecular And Cellular Mr Imaging
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Author : Michel M.J. Modo
language : en
Publisher: CRC Press
Release Date : 2007-03-28
Molecular And Cellular Mr Imaging written by Michel M.J. Modo and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-03-28 with Medical categories.
The ability of molecular and cellular imaging to track the survival, migration, and differentiation of cells in vivo as well as monitor particular gene expression in living subjects is rapidly moving from the research laboratory into daily clinical settings. The interdisciplinary nature of the field mandates a constant dialogue among molecular and
In Vivo Cellular Imaging Using Fluorescent Proteins
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Author : Robert M. Hoffman
language : en
Publisher: Humana Press
Release Date : 2016-05-01
In Vivo Cellular Imaging Using Fluorescent Proteins written by Robert M. Hoffman and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.
This Methods in Molecular Biology book ranges from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes to intravital fluorescent imaging, 3-dimensional imaging and making new far-red and infrared fluorescent proteins."
Fluorescent Materials For Cell Imaging
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Author : Fu-Gen Wu
language : en
Publisher: Springer Nature
Release Date : 2020-10-26
Fluorescent Materials For Cell Imaging written by Fu-Gen Wu and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-26 with Medical categories.
This book focuses on the latest fluorescent materials for cell imaging. Cell imaging is a widely used basic technique that helps scientists gain a better understanding of biological functions through studies of cellular structure and dynamics. In the past decades, the development of a variety of new fluorescent materials has significantly extended the applications of cellular imaging techniques. This book presents recently developed fluorescent materials, including semiconductor quantum dots, carbon dots, silicon nanoparticles, metal nanoclusters, upconversion nanoparticles, conjugated polymers/polymer dots, aggregation-induced emission (AIE) probes, and coordination compounds, used for various cellular imaging purposes. It will appeal to cell biologists and other researchers in academia, industry and clinical settings who are interested in the technical development and advanced applications of fluorescence imaging in cells, tissues and organisms to explore the mechanisms of biological functions and diseases.
Cellular Image Classification
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Author : Xiang Xu
language : en
Publisher: Springer
Release Date : 2016-11-17
Cellular Image Classification written by Xiang Xu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-11-17 with Technology & Engineering categories.
This book introduces new techniques for cellular image feature extraction, pattern recognition and classification. The authors use the antinuclear antibodies (ANAs) in patient serum as the subjects and the Indirect Immunofluorescence (IIF) technique as the imaging protocol to illustrate the applications of the described methods. Throughout the book, the authors provide evaluations for the proposed methods on two publicly available human epithelial (HEp-2) cell datasets: ICPR2012 dataset from the ICPR'12 HEp-2 cell classification contest and ICIP2013 training dataset from the ICIP'13 Competition on cells classification by fluorescent image analysis. First, the reading of imaging results is significantly influenced by one’s qualification and reading systems, causing high intra- and inter-laboratory variance. The authors present a low-order LP21 fiber mode for optical single cell manipulation and imaging staining patterns of HEp-2 cells. A focused four-lobed mode distribution is stable and effective in optical tweezer applications, including selective cell pick-up, pairing, grouping or separation, as well as rotation of cell dimers and clusters. Both translational dragging force and rotational torque in the experiments are in good accordance with the theoretical model. With a simple all-fiber configuration, and low peak irradiation to targeted cells, instrumentation of this optical chuck technology will provide a powerful tool in the ANA-IIF laboratories. Chapters focus on the optical, mechanical and computing systems for the clinical trials. Computer programs for GUI and control of the optical tweezers are also discussed. to more discriminative local distance vector by searching for local neighbors of the local feature in the class-specific manifolds. Encoding and pooling the local distance vectors leads to salient image representation. Combined with the traditional coding methods, this method achieves higher classification accuracy. Then, a rotation invariant textural feature of Pairwise Local Ternary Patterns with Spatial Rotation Invariant (PLTP-SRI) is examined. It is invariant to image rotations, meanwhile it is robust to noise and weak illumination. By adding spatial pyramid structure, this method captures spatial layout information. While the proposed PLTP-SRI feature extracts local feature, the BoW framework builds a global image representation. It is reasonable to combine them together to achieve impressive classification performance, as the combined feature takes the advantages of the two kinds of features in different aspects. Finally, the authors design a Co-occurrence Differential Texton (CoDT) feature to represent the local image patches of HEp-2 cells. The CoDT feature reduces the information loss by ignoring the quantization while it utilizes the spatial relations among the differential micro-texton feature. Thus it can increase the discriminative power. A generative model adaptively characterizes the CoDT feature space of the training data. Furthermore, exploiting a discriminant representation allows for HEp-2 cell images based on the adaptive partitioned feature space. Therefore, the resulting representation is adapted to the classification task. By cooperating with linear Support Vector Machine (SVM) classifier, this framework can exploit the advantages of both generative and discriminative approaches for cellular image classification. The book is written for those researchers who would like to develop their own programs, and the working MatLab codes are included for all the important algorithms presented. It can also be used as a reference book for graduate students and senior undergraduates in the area of biomedical imaging, image feature extraction, pattern recognition and classification. Academics, researchers, and professional will find this to be an exceptional resource.
Imaging And Spectroscopic Analysis Of Living Cells
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Author : P. Michael Conn
language : en
Publisher: Academic Press
Release Date : 2012-02
Imaging And Spectroscopic Analysis Of Living Cells written by P. Michael Conn and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02 with Medical categories.
This volume of Methods in Enzymology is the second of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. Expert authors who are leaders in the field Extensively referenced and useful figures and tables Provides hints and tricks to facilitate reproduction of methods
Imaging In Cellular And Tissue Engineering
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Author : Hanry Yu
language : en
Publisher: CRC Press
Release Date : 2013-05-16
Imaging In Cellular And Tissue Engineering written by Hanry Yu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-05-16 with Medical categories.
Details on specific imaging modalities for different cellular and tissue engineering applications are scattered throughout articles and chapters in the literature. Gathering this information into a single reference, Imaging in Cellular and Tissue Engineering presents both the fundamentals and state of the art in imaging methods, approaches, and app
Imaging Cellular And Molecular Biological Functions
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Author : Spencer L. Shorte
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-09-12
Imaging Cellular And Molecular Biological Functions written by Spencer L. Shorte and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-12 with Science categories.
This book offers a comprehensive selection of essays by leading experts, which covers all aspects of modern imaging, from its application and up-scaling to its development. The chapter content ranges from the basics to the most complex overview of method and protocols. There is ample practical and detailed "how-to" content on important, but rarely addressed topics. This first edition features all-colour-plate chapters, licensed software and a unique, continuously updated website forum.