Cellular Imaging Techniques For Neuroscience And Beyond
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Cellular Imaging Techniques For Neuroscience And Beyond
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Author : Floris G. Wouterlood
language : en
Publisher: Academic Press
Release Date : 2012-12-06
Cellular Imaging Techniques For Neuroscience And Beyond written by Floris G. Wouterlood and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
The imaging of small cellular components requires powerful instruments, and an entire family of equipment and techniques based on the confocal principle has been developed over the past 30 years. Such methods are commonly used by neuroscience researchers, but the majority of these users do not have a microscopy or a cell biology backgrounds and do can encounter difficulties in obtaining and interpreting results. This volume brings experts in high-resolution optical microscopy applications in neuroscience and cell biology together to document the state of the art. Outlining what is currently possible, the volume also discusses promising developments for the future and aids readers in selecting the most scientifically meaningful approach to solve their questions. Each chapter discusses instrumentation and technology in relationship to application in research. All of the common and cutting edge trends are covered - fluorescence / laser electron / nonlinear microscopy, infrared fluorescence, multiphoton imaging, tomography, FRAP, live imaging, STED, PALM/STORM, etc. Single and multiphoton confocal microscopy, and 4-pi confocal microscopy Obtaining nanoresolution via photoactivation localization microscopy (PALM) Several procedures that correlate observations in optical fluorescence microscopy and electron microscopy Study of morphology and function via high-resolution fluorescence procedures Additional high-resolution microscopic techniques
Cellular Imaging Techniques For Neuroscience And Beyond
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Author : Floris G. Wouterlood
language : en
Publisher: Academic Press
Release Date : 2012-08-08
Cellular Imaging Techniques For Neuroscience And Beyond written by Floris G. Wouterlood and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-08-08 with Medical categories.
In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).
Imaging Neurons
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Author : Rafael Yuste
language : en
Publisher:
Release Date : 2000
Imaging Neurons written by Rafael Yuste and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medical categories.
In the past decade, advances in microscopy have been coupled with new methods of culturing and labeling cells to generate the new science of imaging. Imaging technologies allow investigators to look directly inside living cells and probe their form and function in unprecedented detail. This approach is revolutionizing many aspects of biomedical research, particularly neuroscience, in which visual techniques have traditionally been so important. This manual is the first comprehensive description of the range of imaging technologies being applied to living cells. With its origins in a laboratory course taught at Cold Spring Harbor Laboratory by the editors and contributors, it is packed with the kind of technical detail and practical advice that are essential for success, yet seldom found in the research literature. It covers both established methods and cutting-edge techniques such as multiphoton excitation microscopy and imaging of genetically engineered probes. Although it is neurons to which these technologies are most commonly applied, the methods described are readily adaptable to many other cell types. This book will therefore be an invaluable aid to investigators in cell and developmental biology and immunology as well as neuroscience who wish to take advantage of the extraordinary insights into cellular function offered by imaging technologies.
Imaging In Neuroscience And Development
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Author : Rafael Yuste
language : en
Publisher: CSHL Press
Release Date : 2005
Imaging In Neuroscience And Development written by Rafael Yuste and has been published by CSHL Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Brain Imaging
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Author : Najam Arfeen Sharif
language : en
Publisher:
Release Date : 1989
Brain Imaging written by Najam Arfeen Sharif and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989 with Medical categories.
Advanced Optical Methods For Brain Imaging
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Author : Fu-Jen Kao
language : en
Publisher: Springer
Release Date : 2018-05-14
Advanced Optical Methods For Brain Imaging written by Fu-Jen Kao and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-14 with Technology & Engineering categories.
This book highlights the rapidly developing field of advanced optical methods for structural and functional brain imaging. As is known, the brain is the most poorly understood organ of a living body. It is indeed the most complex structure in the known universe and, thus, mapping of the brain has become one of the most exciting frontlines of contemporary research. Starting from the fundamentals of the brain, neurons and synapses, this book presents a streamlined and focused coverage of the core principles, theoretical and experimental approaches, and state-of-the-art applications of most of the currently used imaging methods in brain research. It presents contributions from international leaders on different photonics-based brain imaging modalities and techniques. Included are comprehensive descriptions of many of the technology driven spectacular advances made over the past few years that have allowed novel insights of the structural and functional details of neurons. The book is targeted at researchers, engineers and scientists who are working in the field of brain imaging, neuroscience and connectomics. Although this book is not intended to serve as a textbook, it will appeal to undergraduate students engaged in the specialization of brain imaging.
In Vivo Optical Imaging Of Brain Function Second Edition
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Author : Ron D. Frostig
language : en
Publisher: CRC Press
Release Date : 2009-05-06
In Vivo Optical Imaging Of Brain Function Second Edition written by Ron D. Frostig and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-05-06 with Science categories.
These are exciting times for the field of optical imaging of brain function. Rapid developments in theory and technology continue to considerably advance understanding of brain function. Reflecting changes in the field during the past five years, the second edition of In Vivo Optical Imaging of Brain Function describes state-of-the-art techniques and their applications for the growing field of functional imaging in the live brain using optical imaging techniques. New in the Second Edition: Voltage-sensitive dyes imaging in awake behaving animals Imaging based on genetically encoded probes Imaging of mitochondrial auto-fluorescence as a tool for cortical mapping Using pH-sensitive dyes for functional mapping Modulated imaging Calcium imaging of neuronal activity using 2-photon microscopy Fourier approach to optical imaging Fully updated chapters from the first edition Leading Authorities Explore the Latest Techniques Updated to reflect continuous development in this emerging research area, this new edition, as with the original, reaches across disciplines to review a variety of non-invasive optical techniques used to study activity in the living brain. Leading authorities from such diverse areas as biophysics, neuroscience, and cognitive science present a host of perspectives that range from a single neuron to large assemblies of millions of neurons, captured at various temporal and spatial resolutions. Introducing techniques that were not available just a few years ago, the authors describe the theory, setup, analytical methods, and examples that highlight the advantages of each particular method.
Visualizing Chemistry
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2006-06-01
Visualizing Chemistry written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-06-01 with Science categories.
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Neurohistology And Imaging Techniques
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Author : Radek Pelc
language : en
Publisher: Humana
Release Date : 2020-06-25
Neurohistology And Imaging Techniques written by Radek Pelc and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-25 with Medical categories.
This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Neurohistology and Imaging Techniques is a valuable resource for both expert and novice users of major light microscopic imaging techniques, and those interested in exploring alternate imaging tools.
Adult Neurogenesis Beyond Rats And Mice
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Author : Luca Bonfanti
language : en
Publisher: Frontiers Media SA
Release Date : 2019-02-11
Adult Neurogenesis Beyond Rats And Mice written by Luca Bonfanti and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-11 with categories.
Researchers working on adult neurogenesis have focused largely on inbred laboratory rodents. While this provides a strong advantage of restricting genetic variation in the group, it also narrows our perspective on adult neurogenesis as a biological phenomenon. Many unsolved issues and open questions cannot be resolved without the contribution of comparative studies spanning through widely different species: how did adult neurogenesis evolve, what is the link between adult neurogenesis and brain complexity, how do adult neurogenesis and animal behavior influence each other, how does adult neurogenesis contribute to brain plasticity, cognition and, possibly, repair, and how do experimental conditions affect adult neurogenesis. The main message from the comparative approach to adult neurogenesis is that the relative exclusive focus on laboratory rodents can result in a bias on how we think about this biological process.