Characterization Of Seca N Terminus For Membrane Binding And Activity
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Characterization Of Seca N Terminus For Membrane Binding And Activity
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Author : Jeanetta Holley Floyd
language : en
Publisher:
Release Date : 2007
Characterization Of Seca N Terminus For Membrane Binding And Activity written by Jeanetta Holley Floyd and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Biological transport categories.
SecA is an essential component for the translocation of proteins across bacterial membranes. Though SecA is known to function in the membrane the mechanism for this process remains unclear. In this study we identify two specific regions of SecA that may be important for N-terminal membrane interactions. Molecular modeling of SecA from the E. coli and B. subtilus crystal structures, previously determined, revealed that the first 30 amino acids of SecA consists of a helix of amino acids 1-11 connected by a linker (amino acids 12-16) to an amphipathic helix of amino acids 17-30. The first helix is dispensable for SecA activity; however, deletions in the second N-terminal helix, at amino acids 21-25, result in decrease of SecA activity and a deletion of 26 amino acids no longer complements E. coli ts mutant BL21.19. We show that the deletions of N-terminal amino acids that result in the decrease of SecA activity are correlated to the loss of SecA membrane binding and integration in these deletion mutants. In this study we also test the accuracy of a new membrane protein prediction software PSSM_SVM. This program predicted an embedded membrane (EM) region at SecA amino acids 110-118. The predicted sequence represents an unusual prediction for an EM region as most membrane integral regions consist of 15-30 amino acids. Molecular modeling indicated that the region 110-118 is a part of a helix composed of amino acids 107-121 in E. coli SecA, and is indicative of a membrane embedded domain. Site-directed mutagenesis was carried out with several conserved residues, which included L110, L114, and L118 to determine if substitutions at these positions would affect SecA activity. Our data shows that most SecA mutants (including some predicted to be inactive) are active in vivo; however, L110E and L114R mutations rendered the mutated SecA non-functional. All together this study shows that the N-terminal limit of SecA resides at amino acid 26 and that amino acids 21-25 may form a N-terminal membrane binding determinate. Moreover, the predicted EM region may indeed correspond to a functional embedded membrane region for SecA.
Characterization Of Membrane Associated Seca
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Author : Carl E. Cheadle
language : en
Publisher:
Release Date : 2014
Characterization Of Membrane Associated Seca written by Carl E. Cheadle and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Chemical bonds categories.
Proteins are synthesized in the cytoplasm of bacterial cells and approximately 30 percent of these are exported across the inner membrane and are integrated into the inner membrane or reside in the periplasm or the outer membrane. One system of protein export is the general secretory system which exports precursor proteins through the transmembrane protein complex SecYEG that acts as the selective pore in the inner membrane through which the ATPase SecA translocates the substrate proteins. We have developed an in vitro translocation assay where purified SecYEG and SecA are reconstituted simultaneously into proteoliposomes. Quantifying the number of active translocons in both the proteoliposomes and inverted membrane vesicles with two different precursor proteins revealed that the stoichiometry of SecYEG in the active translocase depends upon the precursor being translocated. We investigated the nature of the interaction between SecA and the membrane using mutant SecA species that lacked the N-terminal ten residues, the C-terminal twenty-one residues, or that lacked the coordinated zinc in the C-terminal twenty-one residues. We observed that SecA species that have the N-terminal ten residues had a higher affinity for the membrane than the SecA deletion mutant SecAdN10 that lacks the ten N-terminal residues. We also identified a role for the N-terminal ten residues of SecA in the translocation process. SecAdN10 is deficient in a step of the translocation process where a 2-kDa segment of the precursor protein is translocated through SecYEG. This deficiency is abolished if a SecA species that contains the N-terminal ten residues is added, and when SecA species that contain the N-terminal ten residues are added to assays with wild-type SecA the rate of the 2-kDa translocation step increases.
Dissertation Abstracts International
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Author :
language : en
Publisher:
Release Date : 2008
Dissertation Abstracts International written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Dissertations, Academic categories.
Protein Movement Across Membranes
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Author : Jerry Eichler
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-02-26
Protein Movement Across Membranes written by Jerry Eichler and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-26 with Science categories.
This book addresses the most recent advances in the transport of proteins across a variety of biological membranes. In addressing this topic, this volume includes several new twists not previously addressed in the literature. In the last few years, the study of protein translocation has been revolutionized by the availability of structural information on many of the components and complexes involved in the process. Unlike earlier books written on protein translocation, this volume considers these advances. In addition, several chapters discuss facets of protein translocation from a systems biology perspective, considered by many to be the next paradigm for biological study. Readers of this book will come away with a deeper understanding of the problems facing researchers of protein translocation and see how the most modern biological techniques and approaches are being recruited to answer those questions. The chapters are also written such that problems awaiting future investigation are clearly presented.
Self Assembling Peptide Systems In Biology Medicine And Engineering
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Author : A. Aggeli
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-05-08
Self Assembling Peptide Systems In Biology Medicine And Engineering written by A. Aggeli and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-05-08 with Science categories.
One ofthe major drivers in biological research is the establishment ofstructures and functions of the 50,000 or so proteins in our bodies. Each has a characteristic- dimensional structure, highly "ordered" yet "disordered"! This structure is essential for a protein's function and, significantly, it must be sustained in the competitive and complex environment of the living cell. It is now being recognised that when a cell loses control, proteins can se- assemble into more complex supermolecular structures such as the amyloid fibres and plaques associated with the pathogenesis of prion (CJD) or age-related (Alzheimer's) diseases. This is a pointer to the wider significance of the self-assembling properties of polypeptides. It has been long known that, in silk, polypeptides are assembled into- sheet structures which impart on the material its highly exploitable properties of flexibility combined with high tensile strength. But only now emerging is the recognition that peptides can Self-assemble into a wide variety of non-protein-like structures, including fibrils, fibres, tubules, sheets and monolayers. These are exciting observations and, more so, the potential for materials and medical exploitations is so wide ranging that over 80 scientists from Europe, USA, Japan and Israel. met 1-6 July 1999 in Crete, to discuss the wide-ranging implications of these novel developments. There was a spirit of excitement about the workshop indicative of an important new endeavor. The emerging perception is that of a new class of materials set to become commercially viable early in the 21st century.
Cumulated Index Medicus
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Author :
language : en
Publisher:
Release Date : 2000
Cumulated Index Medicus written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Medicine categories.
Protein Targeting And Translocation
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Author : D. A. Phoenix
language : en
Publisher:
Release Date : 2016-04-03
Protein Targeting And Translocation written by D. A. Phoenix and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-03 with categories.
Protein targeting is a fast-moving field that has encompassed areas from biophysics to molecular biology to try to gain insight into how proteins are directed to their final functional location and how such macromolecules are able to cross semi-permeable membrane barriers during their journey. This text reviews our current state of knowledge regarding the interaction of proteins at the membrane interface and the assembly of proteins into biological membranes, before proceeding to look at targeting pathways in both prokaryotic and eukaryotic systems. The reviews have been written by some of the leading researchers in the field, with contributions from around the world and with more than 1,800 references. The text is aimed at graduate students and at researchers with an interest in protein targeting, but may also be of use to final-year undergraduates. Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.
The Journal Of Cell Biology
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Author :
language : en
Publisher:
Release Date : 2007
The Journal Of Cell Biology written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Biochemistry categories.
No. 2, pt. 2 of November issue each year from v. 19 (1963)-47 (1970) and v. 55 (1972)- contain the Abstracts of papers presented at the Annual Meeting of the American Society for Cell Biology, 3d (1963)-10th (1970) and 12th (1972)-
Progress In Nucleic Acid Research And Molecular Biology
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Author :
language : en
Publisher: Elsevier
Release Date : 2000-10-19
Progress In Nucleic Acid Research And Molecular Biology written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-10-19 with Science categories.
Nucleic acids are the fundamental building blocks of DNA and RNA and are found in virtually every living cell. Molecular biology is a branch of science that studies the physicochemical properties of molecules in a cell, including nucleic acids, proteins, and enzymes. Increased understanding of nucleic acids and their role in molecular biology will further many of the biological sciences including genetics, biochemistry, and cell biology. Progress in Nucleic Acid Research and Molecular Biology provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references. Provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology Features contributions from leaders in their fields Contains abundant references
Cambridge Scientific Biochemistry Abstracts
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Author :
language : en
Publisher:
Release Date : 1992
Cambridge Scientific Biochemistry Abstracts written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992 with Biochemistry categories.