Chromatography In Biotechnology
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Chromatography In Biotechnology
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Author : Csaba Horváth
language : en
Publisher:
Release Date : 1993
Chromatography In Biotechnology written by Csaba Horváth and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Language Arts & Disciplines categories.
The most up-to-date compilation of significant research on preparative liquid chromatography used for the separation of biomolecules and proteins. Presents recent advances in high-performance liquid chromatographic techniques for the isolation and purification of bioproducts in the laboratory and manufacturing plant. Discusses novel approaches to the preparative/process chromatography of complex carbohydrate and glycoconjugates. Also describes recent advances in column materials.
High Performance Liquid Chromatography In Biotechnology
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Author : William S. Hancock
language : en
Publisher: Wiley-Interscience
Release Date : 1990-10-24
High Performance Liquid Chromatography In Biotechnology written by William S. Hancock and has been published by Wiley-Interscience this book supported file pdf, txt, epub, kindle and other format this book has been release on 1990-10-24 with Science categories.
High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock This important book details the broad range of analytical techniques that have made HPLC so successful in the separation and quantitation of product variants-a procedure essential to quality assurance of protein pharmaceuticals. Outlined are the major chromatographic techniques-reversed phase, ion exchange, affinity and hydrophobic interaction chromatography-as well as their role in specific separations. Also described are the methods required for a quality control program. Reflecting the heightened concern for product integrity since the advent of new protein pharmaceuticals by recombinant-DNA techniques, this critical reference will enhance the libraries of analytical chemists, biochemists, and chemical engineers working with HPLC. 1990 (0-471-82584-0) 624 pp.
Chromatographic And Membrane Processes In Biotechnology
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Author : C.A. Costa
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Chromatographic And Membrane Processes In Biotechnology written by C.A. Costa and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
Separation processes in biotechnology are of increasing industrial importance since they entail the major costs of bioprocessing especially when high purity is required. Chromatography and membranes are two of the most important technologies used for direct treatment of fermentation broths as well as for high resolution steps in product purification. The theoretical foundations of chromatographic and membrane processes are well understood for the case of small molecules. Nevertheless there is a need to adapt and further develop that knowledge to the processing of large biological molecules. This is being achieved with the contribution of other areas like molecular biology and materials science. The objective of this NATO Advanced Study Institute is to present an updated treatment of the fundamentals of chromatographic and membrane processes with special relevance in bioprocessing.This volume collects the lectures presented at this Institute. The lectures are arranged in five chapters. Chapter I deals with chromatographic processes covering topics like equilibrium, kinetics and contacting devices. Membrane processes and some applications in biotechnology are treated in chapter 2. Chapter 3 is devoted to affinity chromatographic and membrane processes. Chapter 4 considers the current developments on chromatographic supports and membranes both from the constitutive materials and form points of view. Scale-up, optimization and reaction/separation integration are the topics covered in chapter 5. We are very grateful to all lecturers and participants that made possible this Institute. Financial support from NATO Scientific Affairs Division, INIC, JNICT, FLAD, University of Ac;ores and DRT Ac;ores is gratefully acknowledged.
High Performance Liquid Chromatography
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Author : Elena Katz
language : en
Publisher: John Wiley & Sons
Release Date : 1996-04-11
High Performance Liquid Chromatography written by Elena Katz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-04-11 with Science categories.
This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Protein Chromatography
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Author : Giorgio Carta
language : en
Publisher: John Wiley & Sons
Release Date : 2020-06-02
Protein Chromatography written by Giorgio Carta and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-06-02 with Science categories.
An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.
High Performance Liquid Chromatography Principles And Methods In Biotechnology
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Author : Ed: Elena D. Katz
language : en
Publisher: John Wiley & Sons
Release Date : 1996
High Performance Liquid Chromatography Principles And Methods In Biotechnology written by Ed: Elena D. Katz and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Biotechnology categories.
This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Modern Advances In Chromatography
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Author : Ruth Freitag
language : en
Publisher: Springer
Release Date : 2003-07-01
Modern Advances In Chromatography written by Ruth Freitag and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-07-01 with Science categories.
Chromatography is the separation method for biological molecules from the analytical to the preparative scale. Few of the recent advances in life science, whether in "proteomics" or in the industrial production of recombinant therapeutics, would have been possible without the help of chromatographic separations. Concomitantly, chromatography nowadays stands for much more than a batch column packed with porous particles. In this book eminent experts from academia and industry introduce the reader to some of the recent new developments in this exciting area. Ranging from evolving nanoanalytical techniques to advances in the material sciences and artificial antibodies and finally continuous large scale separations of the most fragile biologicals, this book should provide interesting reading material for students and practitioners from various fields.
Applications Of Ion Chromatography For Pharmaceutical And Biological Products
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Author : Lokesh Bhattacharyya
language : en
Publisher: John Wiley & Sons
Release Date : 2012-02-10
Applications Of Ion Chromatography For Pharmaceutical And Biological Products written by Lokesh Bhattacharyya and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-10 with Medical categories.
This is a comprehensive source of information on the application of ion chromatography (IC) in the analysis of pharmaceutical drugs and biologicals. This book, with contributors from academia, pharma, the biotech industry, and instrument manufacturing, presents the different perspectives, experience, and expertise of the thought leaders of IC in a comprehensive manner. It explores potential IC applications in different aspects of product development and quality control testing. In addition, an appendix section gives information on critical physical and chromatographic parameters related to IC and information on current manufacturers of IC systems, columns, and other components.
Preparative Chromatography For Separation Of Proteins
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Author : Arne Staby
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-02
Preparative Chromatography For Separation Of Proteins written by Arne Staby and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-02 with Technology & Engineering categories.
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Affinity Chromatography
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Author : Michael Zachariou
language : en
Publisher: Springer Science & Business Media
Release Date : 2008
Affinity Chromatography written by Michael Zachariou and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Medical categories.
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.