Preparative Chromatography For Separation Of Proteins
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Preparative Chromatography For Separation Of Proteins
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Author : Arne Staby
language : en
Publisher: John Wiley & Sons
Release Date : 2017-03-06
Preparative Chromatography For Separation Of Proteins written by Arne Staby and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-03-06 with Technology & Engineering categories.
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Preparative Liquid Chromatography
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Author : B.A. Bidlingmeyer
language : en
Publisher: Elsevier
Release Date : 1987-07-01
Preparative Liquid Chromatography written by B.A. Bidlingmeyer and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-07-01 with Science categories.
This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.
Hplc Of Peptides And Proteins
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Author : Marie-Isabel Aguilar
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Hplc Of Peptides And Proteins written by Marie-Isabel Aguilar and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Preparative Chromatography Techniques
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Author : K. Hostettmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14
Preparative Chromatography Techniques written by K. Hostettmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Science categories.
Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.
A Practical Handbook Of Preparative Hplc
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Author : Donald A. Wellings
language : en
Publisher: Elsevier Science Limited
Release Date : 2006
A Practical Handbook Of Preparative Hplc written by Donald A. Wellings and has been published by Elsevier Science Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Medical categories.
This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA. This book… •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems
Fundamentals Of Preparative And Nonlinear Chromatography
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Author : Georges Guiochon
language : en
Publisher: Academic Press
Release Date : 2006-03-21
Fundamentals Of Preparative And Nonlinear Chromatography written by Georges Guiochon and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-21 with Medical categories.
The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.
Membrane Protein Purification And Crystallization
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Author : Carola Hunte
language : en
Publisher: Elsevier
Release Date : 2003-01-05
Membrane Protein Purification And Crystallization written by Carola Hunte and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-05 with Science categories.
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. - Provides general guidelines and strategies for isolation and crystallization of membrane proteins - Gives detailed protocols that have wide application, and low specialized equipment needs - Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins - Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes - Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization - Presents a comprehensive guide to 2D-crystallization of membrane proteins
Protein Purification
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Author : Jan-Christer Janson
language : en
Publisher: John Wiley & Sons
Release Date : 1998
Protein Purification written by Jan-Christer Janson and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998 with Medical categories.
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Liquid Chromatography
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Author : Salvatore Fanali
language : en
Publisher: Elsevier
Release Date : 2017-06-23
Liquid Chromatography written by Salvatore Fanali and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-23 with Science categories.
Liquid Chromatography: Applications, Second Edition,is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their knowledge of the wide variety of applications in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making
Ion Exchange Chromatography Of Proteins
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Author : Shuichi Yamamoto
language : en
Publisher: CRC Press
Release Date : 1988-04-14
Ion Exchange Chromatography Of Proteins written by Shuichi Yamamoto and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988-04-14 with Science categories.
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.