Preparative Chromatography Techniques
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Preparative Chromatography Techniques
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Author : Kurt Hostettmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Preparative Chromatography Techniques written by Kurt Hostettmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Science categories.
Over the last few years several new instrumental techniques have been introduced for chromatographie separations. In addition, rapid developments in existing methods, such as preparative HPLC, have taken place. Despite these advances, how ever, a handbook covering the various preparative aspects ofthe new separation tech niques does not exist. This book is an attempt to fill the gap and to present a compila tion of modern separation techniques that will be useful for researchers faced with day-to-day preparative problems. Numerous examples of separations have been selected in order to show the possibilities (and also the limits) of each technique treated. These are often either applications from our own laboratory or else they reflect the approach we have been following for the isolation of natural products from plant sourees. Owing to the large number of published papers and the diversity of secondary plant constituents. an exhaustive survey of the literature has not been undertaken. We hope, however, that the examples selected will suggest to the reader which technique(s) and which conditions to choose for a particular isolation problem in the field of natural products. For invaluable help in the preparation of the manuscript for this book, we would like to thank Corinne Appolonia and Christine Marston. Lausanne, November 1985 K. Hostettmann M. Hostettmann A . . Marston Foreword Although not many people realize this, isolation and purification of biologically active materials is becoming increasingly crucial.
Preparative Chromatography Techniques
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Author : K. Hostettmann
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-14
Preparative Chromatography Techniques written by K. Hostettmann and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-14 with Science categories.
Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.
Preparative Liquid Chromatography
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Author : B.A. Bidlingmeyer
language : en
Publisher: Elsevier
Release Date : 1987-07-01
Preparative Liquid Chromatography written by B.A. Bidlingmeyer and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1987-07-01 with Science categories.
This volume provides a straightforward approach to isolation and purification problems with a thorough presentation of preparative LC strategy including the interrelationship between the input and output of the instrumentation, while keeping to an application focus.The book stresses the practical aspects of preparative scale separations from TLC isolations through various laboratory scale column separations to very large scale production. It also gives a thorough description of the performance parameters (e.g. throughput, separation quality, etc.) as a function of operational parameters (e.g. particle size, column size, solvent usage, etc.). Experts in the field have contributed a well balanced presentation of separation development strategies from preparative TLC to commercial preparative process with practical examples in a wide variety of application areas such as drugs, proteins, nucleotides, industrial extracts, organic chemicals, enantiomers, polymers, etc.
Preparative Chromatography For Separation Of Proteins
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Author : Arne Staby
language : en
Publisher: John Wiley & Sons
Release Date : 2017-02-02
Preparative Chromatography For Separation Of Proteins written by Arne Staby and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-02-02 with Technology & Engineering categories.
Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Preparative And Production Scale Chromatography
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Author : G. Ganetsos
language : en
Publisher: CRC Press
Release Date : 1992-09-16
Preparative And Production Scale Chromatography written by G. Ganetsos and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1992-09-16 with Science categories.
Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.
A Practical Handbook Of Preparative Hplc
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Author : Donald A. Wellings
language : en
Publisher: Elsevier Science Limited
Release Date : 2006
A Practical Handbook Of Preparative Hplc written by Donald A. Wellings and has been published by Elsevier Science Limited this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Medical categories.
This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA. This book… •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems •Provides practical, hands-on advice based on years of experience •Will help ensure optimal design, equipment and separation results for your particular task •Presents system layouts from laboratory to process scale •Will help you to devise or improve record-keeping and documentation systems
Techniques And Practice Of Chromatography
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Author : Raymond P.W. Scott
language : en
Publisher: CRC Press
Release Date : 2020-01-29
Techniques And Practice Of Chromatography written by Raymond P.W. Scott and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-29 with Science categories.
This work introduces scientists of all disciplines to the chromatographic process and how it functions. The basic principles of chromatographic separation and specific chromatographic procedures, including gas, liquid and thin-layer chromatography, are covered. For each separation method the book details its characteristics, the instrumentation required, the procedures necessary for effective use, areas of application and examples of its use.;This work is intended for analytical chemists, laboratory technicians, and upper-level undergraduate and graduate students in analytical chemistry or separation science courses.
Fundamentals Of Preparative And Nonlinear Chromatography
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Author : Georges Guiochon
language : en
Publisher: Academic Press
Release Date : 2006-03-21
Fundamentals Of Preparative And Nonlinear Chromatography written by Georges Guiochon and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-21 with Medical categories.
The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.
Principles And Techniques Of Biochemistry And Molecular Biology
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Author : Keith Wilson
language : en
Publisher: Cambridge University Press
Release Date : 2010-03-04
Principles And Techniques Of Biochemistry And Molecular Biology written by Keith Wilson and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-04 with Medical categories.
Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.
Natural Products Isolation
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Author : Satya D. Sarker
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Natural Products Isolation written by Satya D. Sarker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
The term “natural products” spans an extremely large and diverse range of chemical compounds derived and isolated from biological sources. Our interest in natural products can be traced back thousands of years for their usefulness to humankind, and this continues to the present day. Compounds and extracts derived from the biosphere have found uses in medicine, agriculture, cosmetics, and food in ancient and modern societies around the world. Therefore, the ability to access natural products, understand their usefulness, and derive applications has been a major driving force in the field of natural product research. The first edition of Natural Products Isolation provided readers for the first time with some practical guidance in the process of extraction and isolation of natural products and was the result of Richard Cannell’s unique vision and tireless efforts. Unfortunately, Richard Cannell died in 1999 soon after completing the first edition. We are indebted to him and hope this new edition pays adequate tribute to his excellent work. The first edition laid down the “ground rules” and established the techniques available at the time. Since its publication in 1998, there have been significant developments in some areas in natural product isolation. To capture these developments, publication of a second edition is long overdue, and we believe it brings the work up to date while still covering many basic techniques known to save time and effort, and capable of results equivalent to those from more recent and expensive techniques.