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Cryo Em Part A Sample Preparation And Data Collection


Cryo Em Part A Sample Preparation And Data Collection
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Cryo Em Part A Sample Preparation And Data Collection


Cryo Em Part A Sample Preparation And Data Collection
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30

Cryo Em Part A Sample Preparation And Data Collection written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.


Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide



Cryo Em


Cryo Em
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Author : Grant J. Jensen
language : en
Publisher:
Release Date : 2010

Cryo Em written by Grant J. Jensen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with Electron microscopy categories.




Challenges And Solutions In Sample Preparation For High Resolution Cryo Electron Microscopy


Challenges And Solutions In Sample Preparation For High Resolution Cryo Electron Microscopy
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Author : Felix Weis
language : en
Publisher: Frontiers Media SA
Release Date : 2022-12-05

Challenges And Solutions In Sample Preparation For High Resolution Cryo Electron Microscopy written by Felix Weis and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-12-05 with Science categories.




Single Particle Cryo Electron Microscopy


Single Particle Cryo Electron Microscopy
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Author : Joachim Frank
language : en
Publisher: World Scientific Publishing Company
Release Date : 2017-12-31

Single Particle Cryo Electron Microscopy written by Joachim Frank and has been published by World Scientific Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-31 with Electron microscopy categories.


The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.



Serpin Structure And Evolution


Serpin Structure And Evolution
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-11-07

Serpin Structure And Evolution written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-11-07 with Science categories.


Serpins are a group of proteins with similar structures that were first identified as a set of proteins able to inhibit proteases. This volume in the Methods in Enzymology series comprehensively covers this topic. With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems. - This volume in the Methods in Enzymology series comprehensively covers the topic of serpins - With an international board of authors, this volume covers subjects such as Crystallography of serpins and serpin complexes, Serpins as hormone transporters, and Production of serpins using cell free systems



Introduction To Biological Imaging


Introduction To Biological Imaging
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Author : Manfred Auer
language : en
Publisher: John Wiley & Sons
Release Date : 2024-06-10

Introduction To Biological Imaging written by Manfred Auer and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-10 with Medical categories.


Introduction to Biological Imaging Discover what biological imaging is able to accomplish in this up-to-date textbook One of the fundamental goals of biology is to understand how living organisms establish and maintain their spatiotemporal organization of the biochemical, cell biological and developmental biology processes that sustain life. Biological systems are inherently complex with a large number of components needed to sustain cellular function. In order to understand any complex system, one must determine its composition by identifying the components it is made of, how each of these components function and carry out their specific task, and how they interact with one another to function together. To grasp the link of such changes to physiological cell and tissue function and/or pathogenesis/disease progression, we need to understand how modifications alter macromolecular function, macromolecular interactions, and/or spatiotemporal distribution and overall supramolecular structural organization. Biological imaging holds the key to understanding spatiotemporal organization, and will thus be increasingly important for the next generations of biological and biochemical researchers. Introduction to Biological Imaging provides the first comprehensive textbook surveying this subject. It elucidates the fundamental principles underlying the capture and production of bioimages, the requirements of image analysis and interpretation, and some key problems and solutions in bioimaging. It includes everything experimental biologists need to incorporate appropriate bioimaging solutions into their work. Introduction to Biological Imaging readers will also find: Coverage of all major types of biological imaging, including medical imaging, cellular imaging, macromolecular imaging, and more Advice on preparing samples for various imaging methods Specific examples in each chapter connecting bioimaging process to the production of real experimental data Introduction to Biological Imaging is a valuable introduction for undergraduate or graduate students in courses relating to bioimaging, as well as scientists and researchers in the biological and medical fields who want a one-stop reference for the full range of imaging techniques.



Cryo Em Part C


Cryo Em Part C
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30

Cryo Em Part C written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.


This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide



Cryo Electron Microscopy In Structural Biology


Cryo Electron Microscopy In Structural Biology
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Author : Krishnarao Appasani
language : en
Publisher: CRC Press
Release Date : 2024-10-17

Cryo Electron Microscopy In Structural Biology written by Krishnarao Appasani and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-17 with Science categories.


Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics. Key Features • Focuses on the applications of cryo-EM to structural biology • Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry • Reviews the applications of high-resolution structures of viruses • Emphasizes structural insights of nuclear and gene machineries • Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development



Methods In Molecular Biophysics


Methods In Molecular Biophysics
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Author : Igor N. Serdyuk
language : en
Publisher: Cambridge University Press
Release Date : 2017-05-18

Methods In Molecular Biophysics written by Igor N. Serdyuk and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-05-18 with Medical categories.


A comprehensive graduate textbook explaining key physical methods in biology, reflecting the very latest research in this fast-moving field.



Imaging And Spectroscopic Analysis Of Living Cells


Imaging And Spectroscopic Analysis Of Living Cells
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-03-30

Imaging And Spectroscopic Analysis Of Living Cells written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-30 with Science categories.


This volume of Methods in Enzymology is the third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. - The third of 3 parts looking at current methodology for the imaging and spectroscopic analysis of live cells - The chapters provide hints and tricks not available in primary research publications - It is an invaluable resource for academics, researchers and students alike