Cryo Em Part B 3 D Reconstruction
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Cryo Em Part B 3 D Reconstruction
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-30
Cryo Em Part B 3 D Reconstruction written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-30 with Science categories.
This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied. - Includes time-tested core methods and new innovations applicable to any researcher - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide
The Resolution Revolution Recent Advances In Cryoem
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Author :
language : en
Publisher: Academic Press
Release Date : 2016-08-26
The Resolution Revolution Recent Advances In Cryoem written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-26 with Science categories.
cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers research methods that determine the structures of biological molecules, a vital step for understanding their function - Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted
Macromolecular Crystallography
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Author : Maria Armenia Carrondo
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-12-01
Macromolecular Crystallography written by Maria Armenia Carrondo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-01 with Science categories.
This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
Research On Nitrification And Related Processes Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-06-15
Research On Nitrification And Related Processes Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Science categories.
The global nitrogen cycle is the one most impacted by mankind. The past decade has changed our view on many aspects of the microbial biogeochemical cycles, including the global nitrogen cycle, which is mainly due to tremendous advances in methods, techniques and approaches. Many novel processes and the molecular inventory and organisms that facilitate them have been discovered only within the last 5 to 10 years, and the process is in progress. Research on Nitrification and Related Processes, Part B provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments. - Provides state-of-the-art update on methods and protocols - Deals with the detection, isolation and characterization of macromolecules and their hosting organisms - Deals with the challenges of very diverse environments
Constitutive Activity In Receptors And Other Proteins Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2010-11-24
Constitutive Activity In Receptors And Other Proteins Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-24 with Science categories.
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods
Cryo Electron Microscopy In Structural Biology
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Author : Krishnarao Appasani
language : en
Publisher: CRC Press
Release Date : 2024-10-17
Cryo Electron Microscopy In Structural Biology written by Krishnarao Appasani and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-10-17 with Science categories.
Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics. Key Features • Focuses on the applications of cryo-EM to structural biology • Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry • Reviews the applications of high-resolution structures of viruses • Emphasizes structural insights of nuclear and gene machineries • Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development
Single Particle Cryo Electron Microscopy
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Author : Joachim Frank
language : en
Publisher: World Scientific Publishing Company
Release Date : 2017-12-31
Single Particle Cryo Electron Microscopy written by Joachim Frank and has been published by World Scientific Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-31 with Electron microscopy categories.
The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.
Synthetic Biology Part A
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Author : Chris Voigt
language : en
Publisher: Academic Press
Release Date : 2011-07-08
Synthetic Biology Part A written by Chris Voigt and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-07-08 with Science categories.
Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. - Encompasses a variety of different approaches, methodologies and disciplines - Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation
Protein Engineering For Therapeutics Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-02-23
Protein Engineering For Therapeutics Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-23 with Science categories.
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds - Chapters provide an invaluable resource for academics, researchers and students alike - Iinternational board of authors - This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds
Protein Engineering For Therapeutics Part A
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Author : K. Dane Wittrup
language : en
Publisher: Academic Press
Release Date : 2012-01-11
Protein Engineering For Therapeutics Part A written by K. Dane Wittrup and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-01-11 with Medical categories.
This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds Chapters provide an invaluable resource for academics, researchers and students alike Iinternational board of authors This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds