Cytochrome P450 Protocols
DOWNLOAD
Download Cytochrome P450 Protocols PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Cytochrome P450 Protocols book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Cytochrome P450 Protocols
DOWNLOAD
Author : Ian R. Phillips
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-04
Cytochrome P450 Protocols written by Ian R. Phillips and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-04 with Science categories.
For this second edition of their much praised Cytochrome P450, the editors have collected accounts of the essential core techniques that use the latest methodologies for the investigation of P450s. Highlights include protocols for spectral analysis and purification of P450s, enzymatic assays of P450s and flavin-containing monooxygenases (FMOs), expression of P450s and FMOs in heterologous systems, and the production and use of antipeptide antibodies. Additional chapters contain readily reproducible techniques for the transfection of hepatocytes for gene regulation studies, P450 reporter gene assays, in situ hybridization, and analysis of genetic polymorphisms. Although the emphasis is on P450s of mammalian origin, many of the readily reproducible methods described are suitable for P450s from any source.
Flavoprotein Protocols
DOWNLOAD
Author : Steven K. Chapman
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flavoprotein Protocols written by Steven K. Chapman and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.
Cytochrome P450
DOWNLOAD
Author : Paul R. Ortiz de Montellano
language : en
Publisher: Springer Science & Business Media
Release Date : 2005
Cytochrome P450 written by Paul R. Ortiz de Montellano and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005 with Medical categories.
Since the first edition of this title in 1986, the cytochrome P450 field has completed the transition to a discipline in which structure and mechanism, even regulation and biological function, are dealt with in molecular terms. The present updated edition has dropped some of the chapter topics from the first edition to make room for new or expanded topics. The volume is divided into four sections: model systems; structurally defined bacterial enzymes; structures and mechanisms of membrane-bound P450 enzymes; and regulatory mechanisms and physiological roles of cytochrome P450.
Guide To Cytochromes
DOWNLOAD
Author : David F. V. Lewis
language : en
Publisher: CRC Press
Release Date : 2001-08-23
Guide To Cytochromes written by David F. V. Lewis and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001-08-23 with Medical categories.
Cytochromes are coloured iron-containing proteins that transfer electrons during cellular respiration and photosynthesis. The Cytochrome P450 family of enzymes catalyze reactions whereby water-insoluble drugs or metabolites, that would otherwise reach toxic levels in cell membranes, are rendered suitably water-soluble to leave the cell and be excreted in the urine. Due to the extensive nature of this subject, which is an area of intense scientific interest, the field is rapidly advancing and there is a need for new textbooks to keep abreast of the latest developments. The book fulfils that role in providing a fast-track approach for those coming into the P450 field, either at postgraduate level or in particular within the pharmaceutical industry. A Guide to Cytochrome P450 Structure and Function acts as an adjunct to the previous book Cytochromes P450: Structure, Function and Mechanism. It reviews the current status of the P450 field in terms of our present knowledge and understanding of the enzymes structure and function, including their multiplicity of forms, diversity of substrates, and selectivity. This is brought together with the latest research topics, including pharmacogenetics, regulation, human DMEs, toxicity screening and molecule modeling, to provide a fast-track approach for those new to the field.
The Nucleic Acid Protocols Handbook
DOWNLOAD
Author : Ralph Rapley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-06-29
The Nucleic Acid Protocols Handbook written by Ralph Rapley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-29 with Science categories.
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Pcr Protocols
DOWNLOAD
Author : John M. S. Bartlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Pcr Protocols written by John M. S. Bartlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
Handbook Of Porphyrin Science With Applications To Chemistry Physics Materials Science Engineering Biology And Medicine Volumes 1 5
DOWNLOAD
Author : Karl M Kadish
language : en
Publisher: World Scientific
Release Date : 2010-03-16
Handbook Of Porphyrin Science With Applications To Chemistry Physics Materials Science Engineering Biology And Medicine Volumes 1 5 written by Karl M Kadish and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-03-16 with Science categories.
This is the first set of Handbook of Porphyrin Science.Porphyrins, phthalocyanines and their numerous analogues and derivatives are materials of tremendous importance in chemistry, materials science, physics, biology and medicine. They are the red color in blood (heme) and the green in leaves (chlorophyll); they are also excellent ligands that can coordinate with almost every metal in the Periodic Table. Grounded in natural systems, porphyrins are incredibly versatile and can be modified in many ways; each new modification yields derivatives demonstrated new chemistry, physics and biology, with a vast array of medicinal and technical applications.As porphyrins are currently employed as platforms for study of theoretical principles and applications in a wide variety of fields, the Handbook of Porphyrin Science represents a timely ongoing series dealing in detail with the synthesis, chemistry, physicochemical and medical properties and applications of polypyrrole macrocycles. Professors Karl Kadish, Kevin Smith and Roger Guilard are internationally recognized experts in the research field of porphyrins, each having his own separate area of expertise in the field. Between them, they have published over 1500 peer-reviewed papers and edited more than three dozen books on diverse topics of porphyrins and phthalocyanines. In assembling the new volumes of this unique Handbook, they have selected and attracted the very best scientists in each sub-discipline as contributing authors of the chaptersThis Handbook will prove to be a modern authoritative treatise on the subject as it is a collection of up-to-date works by world-renowned experts in the field. Complete with hundreds of figures, tables and structural formulas, and thousands of literature citations, all researchers and graduate students in this field will find the Handbook of Porphyrin Science an essential, major reference source for many years to come.
Cytochrome P450
DOWNLOAD
Author : Zhengyin Yan
language : en
Publisher: Humana
Release Date : 2021-08-10
Cytochrome P450 written by Zhengyin Yan and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-08-10 with Medical categories.
This collection explores detailed experimental protocols necessary for setting up a variety of in vitro cytochrome P450 (CYP) assays that are vital in selecting drug candidates in a drug discovery pipeline. Major factors affecting drug metabolism include CYP expression levels, kinetic parameters for individual CYP enzymes, CYP inhibition and induction, time-dependent inhibition (TDI), CYP stability, non-CYP stability, UDP-glucuronosyltransferases (UGT) stability, excretion mechanisms, and drug-drug interactions (DDI), all addressed in this volume. Written for the Methods in Pharmacology and Toxicology series, chapters include helpful background information on the in vitro assay, a list of all the materials, reagents, and equipment necessary to carry out the assay, a step-by-step protocol, notes containing common and unexpected experimental problems in the assay, as well as references containing important supplementary reading. Authoritative and practical, Cytochrome P450: In Vitro Methods and Protocols serves as a key guide for researchers in the area of discovery and development of new medicines.
Human Cell Culture Protocols
DOWNLOAD
Author : Joanna Picot
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Human Cell Culture Protocols written by Joanna Picot and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Chemotherapy Protocols And Infusion Sequence
DOWNLOAD
Author : Iago Dillion Lima Cavalcanti
language : en
Publisher: Springer Nature
Release Date : 2022-10-18
Chemotherapy Protocols And Infusion Sequence written by Iago Dillion Lima Cavalcanti and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-10-18 with Medical categories.
This book aims to address the infusion sequence of the main protocols used in the treatment of varied solid cancers. Since an inadequate infusion sequence can compromise the patient's treatment, this work will provide support to professionals working in the field of oncology in assessing each chemotherapy infusion sequence. The introductory chapters present the definition, indication, and the risks and benefits of polypharmacy in cancer therapy, and discuss the importance of drug combination in cancer treatment. Chapter 2 focuses on the challenges and also the toxicity of combination therapy in cancer, while chapter 3 highlights the parameters that must be evaluated before defining the infusion sequence, such as pharmacodynamic and pharmacokinetic profiles, drugs’ stability when diluted or reconstituted, toxicological profile of each drug, among others. The remaining chapters are divided by type of cancer. The content is focused on solid tumors, dividing the chapters according to breast, gastrointestinal, genitourinary, gynecological, head and neck, lung, and neurological cancers. In each chapter the epidemiological profiles, pathophysiology, therapeutic modalities, and the main chemotherapy protocols are addressed, as well as efficacy studies and data on the infusion sequence of each mentioned protocol. This work will be a valuable resource to physicians, nurses, and pharmacists, and may help to improve health service practices.