The Nucleic Acid Protocols Handbook

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The Nucleic Acid Protocols Handbook

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Author : Ralph Rapley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-06-29

The Nucleic Acid Protocols Handbook written by Ralph Rapley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-29 with Science categories.

A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

Handbook Of Nucleic Acid Purification

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Author : Dongyou Liu
language : en
Publisher: CRC Press
Release Date : 2009-01-14

Handbook Of Nucleic Acid Purification written by Dongyou Liu and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-14 with Science categories.

An Indispensable Roadmap for Nucleic Acid Preparation Although Friedrich Miescher described the first isolation of nucleic acid in 1869, it was not until 1953 that James Watson and Francis Crick successfully deciphered the structural basis of DNA duplex. Needless to say, in the years since, enormous advances have been made in the study of nucleic a

Dna Sequencing Ii

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Author : Jan Kieleczawa
language : en
Publisher: Jones & Bartlett Learning
Release Date : 2006

Dna Sequencing Ii written by Jan Kieleczawa and has been published by Jones & Bartlett Learning this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006 with Medical categories.

Dr. Kieleczawa's second volume, DNA Sequencing II: Optimizing the Preparation and Clean-Up, is devoted to the various methods used for extraction, clean-up, quantification, and analysis of DNA. This volume is divided into four comprehensive sections - DNA Purification, Cleanup of DNA Fragments, Storage of DNA, and Quantifying DNA and RNA - and offers the reader an in-depth presentation of DNA technologies. The text also touches upon the many tools and software programs that are found in a typical modern biology laboratory. This fascinating text is a wonderful addition to your molecular biology library.

Peptide Nucleic Acids

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Author : Peter E. Nielsen
language : en
Publisher: Garland Science
Release Date : 2004

Peptide Nucleic Acids written by Peter E. Nielsen and has been published by Garland Science this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Medical categories.

Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.

Current Protocols In Nucleic Acid Chemistry

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Author : Serge L. Beaucage
language : en
Publisher: Current Protocols
Release Date : 2000

Current Protocols In Nucleic Acid Chemistry written by Serge L. Beaucage and has been published by Current Protocols this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000 with Science categories.

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.

Techniques In Molecular Systematics And Evolution

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Author : Rob DeSalle
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-04-01

Techniques In Molecular Systematics And Evolution written by Rob DeSalle and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-01 with Science categories.

The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the

Rna Methodologies

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Author : Robert E. Farrell Jr.
language : en
Publisher: Elsevier
Release Date : 2010-07-22

Rna Methodologies written by Robert E. Farrell Jr. and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-07-22 with Science categories.

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Purification Of Laboratory Chemicals

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Author : W.L.F. Armarego
language : en
Publisher: Butterworth-Heinemann
Release Date : 2009-07-23

Purification Of Laboratory Chemicals written by W.L.F. Armarego and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-23 with Science categories.

A best seller since 1966, Purification of Laboratory Chemicals keeps engineers, scientists, chemists, biochemists and students up to date with the purification of the chemical reagents with which they work, the processes for their purification, and guides readerd on critical safety and hazards for the safe handling of chemicals and processes.The Sixth Edition is updated and provides expanded coverage of the latest chemical products and processing techniques, safety and hazards. The book has been reorganised and is now fully indexed by CAS Registry Numbers. Compounds are now grouped to make navigation easier and literature references for all substances and techniques have been added, and ambiguous alternate names and cross references have been removed. - The only comprehensive chemical purification reference, a market leader since 1966, Amarego delivers essential information for research and industrial chemists, pharmacists and engineers: '... (it) will be the most commonly used reference book in any chemical or biochemical laboratory' (MDPI Journal) - An essential lab practice and proceedures manual. Improves efficiency, results and safety by providing critical information for day-to-day lab and processing work. Improved, clear organization and new indexing delivers accurate, reliable information on processes and techniques of purification along with detailed physical properties. - The Sixth Edition has been reorganised and is fully indexed by CAS Registry Numbers; compounds are now grouped to make navigation easier; literature references for all substances and techniques have been added; ambiguous alternate names and cross references removed; new chemical products and processing techniques are covered; hazards and safety remain central to the book.

Encyclopedia Of Genetics

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Author : Eric C.R. Reeve
language : en
Publisher: Routledge
Release Date : 2014-01-14

Encyclopedia Of Genetics written by Eric C.R. Reeve and has been published by Routledge this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-14 with Reference categories.

The Encyclopedia includes 125 entries, beginning with the origins of genetics including historical background on the work of Gregor Mendel and Charles Darwin, and progressing to the structure of DNA and modern theories such as selfish genes. All branches of genetics are covered, including the genetics of bacteria, viruses, insects, animals and plants, as well as humans. Important topical issues such as the human genome project, bioethics, the law and genetics, genetic disorders, GM crops, and the use of transgenic animals for food and pharmaceutical products are fully surveyed. A section on techniques and biotechnology includes modern methods of analysis, from DNA fingerprinting to the new science of bioinformatics. The articles, all written by specialists, are largely non-mathematical and progress from general concepts to deeper understanding. Each essay is fully referenced, with suggestions for further reading. The text is supplemented by extensive illustrations, tables and a color plate section. The Encyclopedia of Genetics will be a valuable companion for all those working or studying in the various fields of genetical research, and a fascinating reference for all readers with a basic background in biology. Also includes color inserts.

Trends In Mrna Vaccine Research

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Author : Gabor Tamas Szabo
language : en
Publisher: John Wiley & Sons
Release Date : 2025-03-31

Trends In Mrna Vaccine Research written by Gabor Tamas Szabo and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-31 with Medical categories.

The authoritative guide to the revolutionary concept behind the successful Covid-19 vaccines In 'Trends in mRNA Vaccine Research', a team of distinguished researchers delivers a practical and up-to-date discussion of the biochemical and biomedical foundations of mRNA vaccines. They also explore the regulatory and manufacturing conditions required for successful vaccine development and review recent progress in a variety of medical fields, including vaccines against pathogens like SARS-CoV-2, HIV, Plasmodium, Mycobacterium tuberculosis, as well as anticancer vaccines. Volume highlights include: - A historical overview of mRNA vaccine development - Immune responses to mRNA vaccines and the choice between modified or unmodified mRNA vaccines - The use of circular RNA therapeutics, self-replicating RNA viruses, and GMP and up-scaling of mRNA vaccine production - Latest data on mRNA vaccines current in development for tick-borne diseases, malaria and other parasitic diseases, AIDS, and cancer Perfect for medicinal chemists, immunologists, and epidemiologists, 'Trends in mRNA Vaccine Research' will also benefit researchers and scientists working in the pharmaceutical industry, as well as cancer researchers with an interest in vaccine development.