Cytokine Receptor Protocols
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Cytokine Receptor Protocols
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Author : V. Barak
language : en
Publisher:
Release Date : 2002-12-01
Cytokine Receptor Protocols written by V. Barak and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-12-01 with categories.
Cytokine Protocols
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Author : Marc De Ley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Cytokine Protocols written by Marc De Ley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Medical categories.
A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Enjoy a quick and smooth introduction to the key methods used in cytokine research Use readily reproducible techniques that ensure successful experimental results Employ antisense-RNA, RT-PCR of small amounts of mRNA, and in situ hybridization.
Tissue Protective Cytokines
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Author : Pietro Ghezzi
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Tissue Protective Cytokines written by Pietro Ghezzi and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.
Thorough and informative, this Methods in Molecular Biology book presents key models used to characterize the tissue-protective actions of cytokines. Offers lists of materials and reagents, step-by-step laboratory protocols, troubleshooting tips and pitfalls."
Toll Like Receptors
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Author : Claire E. McCoy
language : en
Publisher: Humana Press
Release Date : 2011-01-26
Toll Like Receptors written by Claire E. McCoy and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-01-26 with Medical categories.
In addition to their invaluable role in the fight against infection, Toll-Like Receptors (TLRs) in an under- or over-active state can lead to the pathogenesis of disease, making these receptors a key focal point for many research laboratories. In Toll-Like Receptors: Methods and Protocols, experts in the field contribute techniques currently used to study TLRs, their downstream signalling pathways, and their role in the pathogenesis of disease, with sections examining TLR expression and the application of genetic techniques and microarray analysis to TLR research. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections highlighting tips on troubleshooting and avoiding known pitfalls. Versatile and cutting-edge, Toll-Like Receptors: Methods and Protocols provides an ideal manual for a wide range of biologists and medical researchers studying these vital components of the immune system.
Chemokine Protocols
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Author : Amanda E.I. Proudfoot
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Chemokine Protocols written by Amanda E.I. Proudfoot and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Medical categories.
The chemokines family of small proteins are involved in numerous b- logical processes ranging from hematopoiesis, angiogenesis, and basal l- kocyte trafficking to the extravasation and tissue infiltration of leukocytes in response to inflammatory agents, tissue damage, and bacterial or viral infection. Chemokines exert their effects through a family of seven G-protein coupled transmembrane receptors. Worldwide interest in the chemokine field surged dramatically early in 1996, with the finding that certain chemokine receptors were the elusive coreceptors, required along with CD4, for HIV infection. Today, though over 40 human chemokines have been described, the n- ber of chemokine receptors lags behind—only 17 human chemokine receptors have been identified so far. What has emerged over the years is that most chemokine receptors bind several distinct ligands, and indeed the majority of chemokines are able to bind to multiple chemokine receptors, explaining to some extent the apparent disparity in the numbers of chemokines and rec- tors. Yet in spite of the apparent redundancy in chemokine/chemokine rec- tor interactions, it is clear that in vivo, spatial, temporal, and indeed cell- and tissue-specific expression of both chemokines and their receptors are imp- tant factors in determining the precise nature of cellular infiltrates in phy- ological and pathological processes.
Cytokines And Cytokine Receptors
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Author : Constantin A. Bona
language : en
Publisher: CRC Press
Release Date : 2003-09-02
Cytokines And Cytokine Receptors written by Constantin A. Bona and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-09-02 with Science categories.
The field of cytokine research is expanding at a rapid pace Contributions from the major leading groups in the world on the structure and biological properties of cytokine and cytokine receptors, as well as integrated reviews on cytokines in various physiological and pathological conditions were presented in three issues of International Reviews of Immunology This collection of articles provided a unique source of information However, important discoveries are emerging very rapidly and some of the reviews written in 1997 are already outdated In this book, the editors assemble reviews that have been updated by their authors to include all the recent publications and unpublished data from the authors' laboratories This volume should serve as an excellent reference source for all those concerned by the multiple faces of cytokines in basic research and in the clinic
Human Cytokines And Cytokine Receptors
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Author : Humana Press
language : en
Publisher: Humana Press
Release Date : 2002-01-01
Human Cytokines And Cytokine Receptors written by Humana Press and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-01-01 with Medical categories.
Short Protocols In Immunology
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Author : John E. Coligan
language : en
Publisher: Wiley
Release Date : 2005-05-02
Short Protocols In Immunology written by John E. Coligan and has been published by Wiley this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-05-02 with Science categories.
Short Protocols in Immunology is an easy-to use companion to Current Protocols in Immunology. Drawn from the most fundamental and widely used methods in the well-known parent volume, this book provides step-by-step protocols in their most easily accessible format for use at the bench. Immunologists in basic science and clinical work alike will find techniques they rely on, including production, purification, and fragmentation of antibodies; assays for lymphocyte function; measurement of cytokines and cytokine receptors; biochemistry of lymphocyte activation; innate immunity, isolation of human immune cell populations, detection and analysis of HIV, and animal models for a variety of autoimmune and infectious diseases.
Cytokine Bioassays
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Author : Ivana Vancurova
language : en
Publisher:
Release Date : 2014
Cytokine Bioassays written by Ivana Vancurova and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Biological assay categories.
Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.
Jak Stat Signalling
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Author : Sandra E. Nicholson
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Jak Stat Signalling written by Sandra E. Nicholson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Science categories.
This Methods in Molecular Biology volume covers JAK and STAT specific approaches, the negative regulators of the pathway, and production and crystallization of JAK and STAT proteins, and more.Includes protocols, materials lists, tips and troubleshooting advice."
