Dendritic Cell Protocols
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Dendritic Cell Protocols
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Author : Stephen P. Robinson
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Dendritic Cell Protocols written by Stephen P. Robinson and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But what is a dendritic cell? The explosion of studies on this cell type over the last few years is breathtaking. We now have so-called “myeloid” and “plas- cytoid” dendritic cells; are they of separate lineages or are they related? There are claims that dendritic cells can be derived from B cells and from granulocytes, as well as from “monocytes. ” Lymphocytes can be switched on or off by dendritic cells. Dendritic cells can preferentially cause Th1 or Th2 responses or some may stimulate B cells directly. When there is so much scope for exploring these new developments, it is particularly important that the data that are established and the practical basis for this work are clearly available, a task that this book fulfils.
Dendritic Cell Protocols
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Author : Elodie Segura
language : en
Publisher: Methods in Molecular Biology
Release Date : 2018-05-18
Dendritic Cell Protocols written by Elodie Segura and has been published by Methods in Molecular Biology this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-18 with Medical categories.
The third edition of this volume is aimed at providing both beginners and more experienced researchers a choice of methods to isolate and analyze dendritic cells(DC). An introductory review provides an overview of recent advances in the characterization of DC subsets in mouse and human. While additional chapters provide methods to culture human and mouse dendritic cells, protocols for the isolation of dendritic cells, the isolation of dendritic cell progenitors from mouse, and the purification of dendritic cells from human blood. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Dendritic Cell Protocols, Third Edition aims to ensure successful results in the further study of this vital field.
T Cell Protocols
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Author : Kelly P. Kearse
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
T Cell Protocols written by Kelly P. Kearse and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Medical categories.
The purpose of T Cell Protocols: Development and Activation is to c- lect a series of protocols, particularly those that have been developed within the past few years, to help investigators master new techniques (or improve existing ones) for the study of T-cell Biology. Invariably, in putting together a book like this it is difficult to decide which methods to include and which to leave out. To this end methods were selected from a variety of disciplines, including cellular immunology, b- chemistry, and molecular biology, to try to provide something of interest for everyone who works on T-cell development and activation. I would like to mention that my primary reason for agreeing to put this book together is that, when I was a graduate student, I purchased a copy of Selected Methods in Cellular Immunology by Mishell and Shigii which proved a tremendous help in learning the basics of one-and two- dimensional gel te- niques (and other methods). The cover has long since fallen off, but it still remains one of my most valued reference books for the laboratory. It is my hope that T Cell Protocols: Development and Activation will prove similarly useful to current and future scientists wishing to learn new methods for expl- ing the development and activation of T cells.
Dendritic Cells
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Author : Vanja Sisirak
language : en
Publisher: Springer Nature
Release Date : 2023-03-11
Dendritic Cells written by Vanja Sisirak and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-03-11 with Science categories.
This detailed volume provides methods that can be used to study dendritic cell (DC) ontogeny, isolation, migration, and functions. After an introduction to murine and human DC subsets and their unique transcriptional, phenotypic, and functional properties, the book continues with sections covering in vivo studies, in vitro differentiation, enrichment, functional characterization, as well as Omics approaches to study dendritic cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Dendritic Cells: Methods and Protocols is an ideal guide to familiarize readers with the current state of the art techniques to investigate these vital cells.
The Impact Of Food Bioactives On Health
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Author : Kitty Verhoeckx
language : en
Publisher: Springer
Release Date : 2015-04-29
The Impact Of Food Bioactives On Health written by Kitty Verhoeckx and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-04-29 with Technology & Engineering categories.
“Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Tumor Immunology And Immunotherapy Cellular Methods Part B
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Author :
language : en
Publisher: Academic Press
Release Date : 2020-01-28
Tumor Immunology And Immunotherapy Cellular Methods Part B written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-28 with Science categories.
Tumor Immunology and Immunotherapy - Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.
Dendritic Cell Protocols
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Author : Stephen P. Robinson
language : en
Publisher:
Release Date : 2010-10-28
Dendritic Cell Protocols written by Stephen P. Robinson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-10-28 with Medical categories.
Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.
Signaling By Toll Like Receptors
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Author : Gregory W. Konat
language : en
Publisher: CRC Press
Release Date : 2008-06-20
Signaling By Toll Like Receptors written by Gregory W. Konat and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-06-20 with Medical categories.
The discovery of toll-like receptors (TLRs) spurred the field of innate immunity into a renaissance after many years of neglect. Since then, TLR research has grown at an exponential rate. Taking an integrated methodological approach, Signaling by Toll-Like Receptors offers a comprehensive review of important techniques in molecular biology,
Dendritic Cell Protocols Methods In Molecular Medicine
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Author : Stephen P. Robinson
language : en
Publisher:
Release Date : 2001
Dendritic Cell Protocols Methods In Molecular Medicine written by Stephen P. Robinson and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.
New Trends In Allergy V
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Author : Johannes Ring
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
New Trends In Allergy V written by Johannes Ring and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
New Trends in Allergy V' is the fifth volume of a series focussing on new trends in experimental and clinical allergology. The interdisciplinary character is documented by articles from such different fields as basic immunology, chemistry, molecular biology, pharmacology, epidemiology, pneumology, dermatology, otolaryngology, pediatrics and psychosomatic medicine. Special emphasis is given to the role of both anthropogenic (pollutants) as well as natural environmental factors in the development of allergy. Among new pathophysiological concepts, dendritic cells, lymphocyte interactions, mast cells, eosinophils and neutrophils as well as psycho-neuro-immunological influences are featured. Practical aspects focus on asthma, rhinoconjunctivitis, atopic eczema, occupational and food allergy as well as anaphylaxis. The most recent therapeutic strategies comprise novel pharmacotherapy and allergen-specific as well as unspecific (e. g. anti-IgE) immunotherapy.