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Detection And Typing Strategies For Pathogenic Escherichia Coli


Detection And Typing Strategies For Pathogenic Escherichia Coli
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Detection And Typing Strategies For Pathogenic Escherichia Coli


Detection And Typing Strategies For Pathogenic Escherichia Coli
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Author : Lucia Rivas
language : en
Publisher: Springer
Release Date : 2015-01-28

Detection And Typing Strategies For Pathogenic Escherichia Coli written by Lucia Rivas and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-01-28 with Technology & Engineering categories.


This Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. Pathogenic forms of E. coli can cause a variety of diarrheal diseases in hosts due to the presence of specific colonization and virulence factors, and pathogenicity-associated genes, which are generally not present in other E. coli. Six pathotypes of pathogenic E. coli are recognized (Shiga toxigenic E. coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, Enteroinvasive E. coli, Enteroaggregative E. coli and Diffusely Adherent E. coli) and certain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies.



Ensuring Safety And Quality In The Production Of Beef Volume 1


Ensuring Safety And Quality In The Production Of Beef Volume 1
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Author : Prof. Gary R. Acuff
language : en
Publisher: Burleigh Dodds Science Publishing
Release Date : 2017-06-09

Ensuring Safety And Quality In The Production Of Beef Volume 1 written by Prof. Gary R. Acuff and has been published by Burleigh Dodds Science Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-09 with Technology & Engineering categories.


Reviews current research on the main pathogens affecting beef; Summarises best practice in pathogen detection and safety management on the farm; Discusses methods for ensuring safety in the food chain from slaughter to consumer handling of fresh beef



Methods For Monitoring Mitochondrial Functions During Pathogen Infection


Methods For Monitoring Mitochondrial Functions During Pathogen Infection
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Author :
language : en
Publisher: Elsevier
Release Date : 2025-03-10

Methods For Monitoring Mitochondrial Functions During Pathogen Infection written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-03-10 with Science categories.


Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 194 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization, and applications in CAR T Cells



Emerging Approaches For Typing Detection Characterization And Traceback Of Escherichia Coli 2nd Edition


Emerging Approaches For Typing Detection Characterization And Traceback Of Escherichia Coli 2nd Edition
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Author : Pina M. Fratamico
language : en
Publisher: Frontiers Media SA
Release Date : 2018-05-02

Emerging Approaches For Typing Detection Characterization And Traceback Of Escherichia Coli 2nd Edition written by Pina M. Fratamico and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-05-02 with categories.


Pathogenic Escherichia coli strains cause a large number of diseases in humans, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, urinary tract infections, and neonatal meningitis, while in animals they cause diseases such as calf scours and mastitis in cattle, post-weaning diarrhea and edema disease in pigs, and peritonitis and airsacculitis in chickens. The different E. coli pathotypes are characterized by the presence of specific sets of virulence-related genes. Therefore, it is not surprising that pathogenic E. coli constitutes a genetically heterogeneous family of bacteria, and they are continuing to evolve. Rapid and accurate molecular methods are critically needed to detect and trace pathogenic E. coli in food and animals. They are also needed for epidemiological investigations to enhance food safety, as well as animal and human health and to minimize the size and geographical extent of outbreaks. The serotype of E. coli strains has traditionally been determined using antisera raised against the >180 different O- (somatic) and 53 H- (flagellar) antigens. However, there are many problems associated with serotyping, including: it is labor-intensive and time consuming; cross reactivity of the antisera with different serogroups occurs; antisera are available only in specialized laboratories; and many strains are non-typeable. Molecular serotyping targeting O-group-specific genes within the E. coli O-antigen gene clusters and genes that are involved in encoding for the different flagellar types offers an improved approach for determining the E. coliO- and H-groups. Furthermore, molecular serotyping can be coupled with determination of specific sets of virulence genes carried by the strain offering the possibility to determine O-group, pathotype, and the pathogenic potential simultaneously. Sequencing of the O-antigen gene clusters of all of the known O-groups of E. coli is now complete, and the sequences have been deposited in the GenBank database. The sequence information has revealed that some E. coli serogroups have identical sequences while others have point mutations or insertion sequences and type as different serogroups in serological reactions. There are also a number of other ambiguities in serotyping that need to be resolved. Furthermore, new E. coli O-groups are being identified. Therefore, there is an essential need to resolve these issues and to revise the E. coli serotype nomenclature based on these findings. There are emerging technologies that can potentially be applied for molecular serotyping and detection and characterization of E. coli. On a related topic, the genome sequence of thousands of E. coli strains have been deposited in GenBank, and this information is revealing unique markers such as CRISPR (clustered regularly interspaced short palindromic repeats) and virulence gene markers that could be used to identify E. coli pathotypes. Whole genome sequencing now provides the opportunity to study the role of horizontal gene transfer in the evolution and emergence of pathogenic E. coli strains. Whole genome sequencing approaches are being investigated for genotyping and outbreak investigation for regulatory and public health needs; however, there is a need for establishing bioinformatics pipelines able to handle large amounts of data as we move toward the use of genetic approaches for non-culture-based detection and characterization of E. coli and for outbreak investigations.



Molecular Medical Microbiology


Molecular Medical Microbiology
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Author : Yi-Wei Tang
language : en
Publisher: Academic Press
Release Date : 2023-11-21

Molecular Medical Microbiology written by Yi-Wei Tang and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-11-21 with Science categories.


Molecular Medical Microbiology, Third Edition presents the latest release in what is considered to be the first book to synthesize new developments in both molecular and clinical research. The molecular age has brought about dramatic changes in medical microbiology, along with great leaps in our understanding of the mechanisms of infectious disease. This third edition is completely updated, reviewed and expanded, providing a timely and helpful update for microbiologists, students and clinicians in the era of increasing use of molecular techniques, changing epidemiology and prevalence, and increasing resistance of many pathogenic bacteria. Written by experts in the field, chapters include cutting-edge information and clinical overviews for each major bacterial group, along with the latest updates on vaccine development, molecular technology and diagnostic technology. - Completely updated and revised edition of this comprehensive and accessible reference on molecular medical microbiology - Includes full color presentations throughout - Delves into in-depth discussions on individual pathogenic bacteria in a system-oriented approach - Includes a clinical overview for each major bacterial group - Presents the latest information on vaccine development, molecular technology and diagnostic technology - Provides more than 100 chapters on all major groups of bacteria



Antimicrobial Resistance Monitoring And Surveillance Guidelines For Food Producing Animals And Their Products In Eastern Africa


Antimicrobial Resistance Monitoring And Surveillance Guidelines For Food Producing Animals And Their Products In Eastern Africa
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Author : Food and Agriculture Organization of the United Nations
language : en
Publisher: Food & Agriculture Org. [Author] [Author]
Release Date : 2024-05-22

Antimicrobial Resistance Monitoring And Surveillance Guidelines For Food Producing Animals And Their Products In Eastern Africa written by Food and Agriculture Organization of the United Nations and has been published by Food & Agriculture Org. [Author] [Author] this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-05-22 with Science categories.


This publication is a building block of the Eastern Africa antimicrobial resistance (AMR) surveillance roadmap that was described in April 2019, by AMR experts from Djibouti, Eritrea, Ethiopia, Kenya, Rwanda, South Sudan, Sudan and Uganda. [Author] The roadmap is presented in chapter six of this document. [Author] The national AMR experts came together in a regional meeting organized by the Food and Agriculture Organization of the United Nations (FAO) and also attended by other national, regional and international organizations such as the World Organisation for Animal Health (WOAH), International Livestock Research Institute (ILRI), Centres for Disease Control and Prevention (CDC)-Kenya, University of Nairobi (UON), Kenya Medical Research Institute (KEMRI), World Animal Protection (WAP) and African Union-Interagency Bureau for Animal Resources (AU-IBAR). [Author] The aim of the roadmap is to set out the processes, tools and coordination that technical experts and decision-makers from within national governments in East Africa agreed should be undertaken at regional level to support development and implementation of national AMR surveillance strategies and plans. [Author]



Encyclopedia Of Microbiology


Encyclopedia Of Microbiology
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Author : Thomas M. Schmidt
language : en
Publisher: Academic Press
Release Date : 2019-09-11

Encyclopedia Of Microbiology written by Thomas M. Schmidt and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-09-11 with Science categories.


Encyclopedia of Microbiology, Fourth Edition, Five Volume Set gathers both basic and applied dimensions in this dynamic field that includes virtually all environments on Earth. This range attracts a growing number of cross-disciplinary studies, which the encyclopedia makes available to readers from diverse educational backgrounds. The new edition builds on the solid foundation established in earlier versions, adding new material that reflects recent advances in the field. New focus areas include `Animal and Plant Microbiomes’ and ‘Global Impact of Microbes`. The thematic organization of the work allows users to focus on specific areas, e.g., for didactical purposes, while also browsing for topics in different areas. Offers an up-to-date and authoritative resource that covers the entire field of microbiology, from basic principles, to applied technologies Provides an organic overview that is useful to academic teachers and scientists from different backgrounds Includes chapters that are enriched with figures and graphs, and that can be easily consulted in isolation to find fundamental definitions and concepts



Trending Topics In Escherichia Coli Research


Trending Topics In Escherichia Coli Research
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Author : Alfredo G. Torres
language : en
Publisher: Springer Nature
Release Date : 2023-06-28

Trending Topics In Escherichia Coli Research written by Alfredo G. Torres and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-06-28 with Science categories.


The Latin American region has been at the forefront in combating infections caused by Escherichia coli strains in humans, animals, and the environment. The continuous emergence and evolution of pathogenic E. coli strains associated with human and animal infections have demonstrated that (i) groups of related pathogenic E. coli are responsible for most infections caused by this bacterial species; (ii) diverse virulence phenotypes expressed during infection defined each one of these pathogroups; (iii) the geographical distribution of pathogroups in Latin America and the evolution of new isolates was defined by the dominant pathogroup and presence of distinct virulence strains; (iv) acquisition of mobile elements or accumulation of point mutations accelerate the development of antibiotic resistance in some of these strains. The Latin American Coalition for Escherichia coli Research (LACER), a multidisciplinary network of over seventy research groups in eleven Latin American countries and the USA, was established in 2009 to apply One Health principles in defining and combating this pathogen. The previous edition of this text, Escherichia coli in the Americas (c. 2016) was the culmination of their wisdom about E. coli, from its role as a commensal bacterium to its characteristics as a pathogen causing disease in animals and humans. This new edition presents recent advances and contextualizes all aspects of E. coli in a One Health perspective, from the environment, to animals, to humans. It addresses E. coli interactions with host microbiota, CNS, and phages. It includes cutting edge insights on hybrid strains, molecular mechanisms of virulence and pathogenesis, resistance, domestic and wild reservoirs, disease surveillance in Latin America, genomic analysis, food safety, and new therapies. Crucially, it also provides translations and analysis of key reports on Escherichia coli published in Spanish and Portuguese. This book serves as a critical resource for scientists in industry and academia, clinicians managing associated infections, and trainees and students studying basic and clinical aspects of E. coli pathogenesis.



Conference Research Topic 9th Symposium On Antimicrobial Resistance In Animals And The Environment Arae 2023


Conference Research Topic 9th Symposium On Antimicrobial Resistance In Animals And The Environment Arae 2023
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Author : Benoit Doublet
language : en
Publisher: Frontiers Media SA
Release Date : 2025-02-03

Conference Research Topic 9th Symposium On Antimicrobial Resistance In Animals And The Environment Arae 2023 written by Benoit Doublet and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2025-02-03 with Science categories.


The emergence of antimicrobial resistance is a seminally important public health concern. Significant progress has been made in recent years regarding an understanding of the genetic and biochemical basis for antimicrobial resistance, the emergence of resistance genes, and factors promoting their widespread dissemination including the role of lateral gene transfer. Nevertheless, there is a dearth of information regarding the key ‘hotspots’ and genetic mechanisms responsible for resistance development, and the exposure routes leading to the failure of antimicrobial agents important in human and animal medicine. There is thus an urgent need for research to provide governments, public health stakeholders, and the agricultural sector the knowledge required to develop policies and practices that effectively mitigate resistance development. This, within a growing recognition that humans, animals and the environment must be considered as intimately linked together if any resistance management strategy is to be successful.



Biosensing Technologies For The Detection Of Pathogens


Biosensing Technologies For The Detection Of Pathogens
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Author : Toonika Rinken
language : en
Publisher: BoD – Books on Demand
Release Date : 2018-03-21

Biosensing Technologies For The Detection Of Pathogens written by Toonika Rinken and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-21 with Medical categories.


Rapid multiplex detection of pathogens in the environment and in our food is a key factor for the prevention and effective treatment of infectious diseases. Biosensing technologies combining the high selectivity of biomolecular recognition and the sensitivity of modern signal detection platforms are a prospective option for automated analyses. They allow rapid detection of single molecules as well as cellular substances. This book, including 12 chapters from 50 authors, introduces the principles of identification of specific pathogen biomarkers along with different biosensor-based technologies applied for pathogen detection.