Development Of Photonic Crystal Biosensor For Label Free Bioassays
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Development Of Photonic Crystal Biosensor For Label Free Bioassays
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Author : Syed Hussain
language : en
Publisher:
Release Date : 2011
Development Of Photonic Crystal Biosensor For Label Free Bioassays written by Syed Hussain and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Biological assay categories.
Study And Development Of Label Free Optical Biosensors For Biomedical Applications
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Author : Charles J. Choi
language : en
Publisher:
Release Date : 2011
Study And Development Of Label Free Optical Biosensors For Biomedical Applications written by Charles J. Choi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
For the majority of assays currently performed, fluorescent or colorimetric chemical labels are commonly attached to the molecules under study so that they may be readily visualized. The methods of using labels to track biomolecular binding events are very sensitive and effective, and are employed as standardized assay protocol across research labs worldwide. However, using labels induces experimental uncertainties due to the effect of the label on molecular conformation, active binding sites, or inability to find an appropriate label that functions equivalently for all molecules in an experiment. Therefore, the ability to perform highly sensitive biochemical detection without the use of fluorescent labels would further simplify assay protocols and would provide quantitative kinetic data, while removing experimental artifacts from fluorescent quenching, shelf-life, and background fluorescence phenomena. In view of the advantages mentioned above, the study and development of optical label-free sensor technologies have been undertaken here. In general, label-free photonic crystal (PC) biosensors and metal nanodome array surface-enhanced Raman scattering (SERS) substrates, both of which are fabricated by nanoreplica molding process, have been used as the method to attack the problem. Chapter 1 shows the work on PC label-free biosensor incorporated microfluidic network for bioassay performance enhancement and kinetic reaction rate constant determination. Chapter 2 describes the work on theoretical and experimental comparison of label-free biosensing in microplate, microfluidic, and spot-based affinity capture assays. Chapter 3 shows the work on integration of PC biosensor with actuate-to-open valve microfluidic chip for pL-volume combinatorial mixing and screening application. In Chapter 4, the development and characterization of SERS nanodome array is shown. Lastly, Chapter 5 describes SERS nanodome sensor incorporated tubing for point-of-care monitoring of intravenous drugs and metabolites.
Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms
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Author : Rashmi Sriram
language : en
Publisher:
Release Date : 2015
Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms written by Rashmi Sriram and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
"Optical label-free biosensors promise high-throughput, rapid, and sensitive detection of biomolecules. As such, these biodetection platforms have applications in clinical diagnostics, pharmaceutical industry, homeland security and in environmental monitoring. Motivated by these attributes, my thesis has focused on advancing the biosensing technology of two novel silicon-based optical label-free sensors: arrayed imaging reflectometry (AIR) and two-dimensional photonic crystal structures (2D PhC). The AIR biosensor is an interferometry-based technique that relies on the creation and destruction of a near-zero reflectivity condition in a carefully chosen thin film. To thoroughly quantify the biosensor detection signal, a correlation between the sensor response and the solution analyte concentration needs to be determined. In the first half of this thesis, a theoretical framework was developed to predict the AIR sensor response for a given solution analyte concentration. The developed model was subsequently validated using spectroscopic ellipsometry and surface plasmon resonance techniques in addition to AIR by utilizing two model probe-analyte pairs. It was established that the probe-analyte interaction on AIR substrate across two formats: macrospots and microarrays closely represented one-site and two-site Langmuir models, respectively. Two-dimensional photonic crystals are periodic dielectric structures with spatial periodicity in two dimensions. In the presence of a "defect" microcavity that disrupts the spatial periodicity, light of specific wavelength can be captured within the defect cavity. This manifests as a "dip" in the transmission spectrum of the crystal and is very sensitive to the local refractive index changes in the immediate vicinity of the defect cavity. The second half of this thesis investigates the utility of two designs of the 2D PhC: "small" and "large" point-defect microcavities coupled to a transmission waveguide for sensing latex particles in microfluidic environment. With the "small" defect design, detection of ~100 nm size latex particles in microfluidic environment was confirmed. This is the first study that demonstrates particle detection in the presence of a fluid cover using a 2D PhC sensor, a significant technological advancement for the incorporation of this sensor into lab-on-a-chip device. Using a "large" point-defect cavity design, real-time detection of latex particles of size ~280 nm was demonstrated. This to our knowledge is the first demonstration of detection of latex particles in real-time using 2D PhCs without the aid of any optical forces. This proof-of-principle demonstration of particle detection lays the foundation for the further development of these devices for the detection of a single biological particle, which when accomplished will have a huge impact in the field of clinical diagnostics"--Pages ix-x.
Biophotonics And Biosensing
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Author : Andrea Armani
language : en
Publisher: Elsevier
Release Date : 2024-06-03
Biophotonics And Biosensing written by Andrea Armani and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2024-06-03 with Technology & Engineering categories.
Biophotonics and Biosensing: From Fundamental Research to Clinical Trials Through Advances of Signal and Image Processing brings together the knowledge of the basic principles of the field of light-biological tissue interaction, detection methods, data processing techniques, and research, diagnostic and clinical applications. It is suitable for new entrants, while also highlighting the latest developments for experts in the field. This volume includes perspectives by leading experts from the biophotonics, biomedical engineering, and data science communities. The reader will receive a basic grounding in the key theoretical principles and practical components of biophotonics and biosensing. Working principles of devices used in spectroscopy, microscopy, and optical sensing are presented along with their application domains. The reader will learn about existing microscopy-based techniques used in biomedical applications for diagnosis and get to know different signal processing algorithms as used in biophotonics. Finally, through concrete examples, including sample preparation and measurement approaches, see how the field has developed thanks to the integration of biophotonics and optical biosensing with signal processing. Introduces key principles of light-biological tissue interactions and biosensing Discusses how the most promising optical diagnostic methods can exploit contemporary signal and image processing algorithms and data analytics Includes examples of clinical studies with detailed descriptions of their implementation, along with practical guidance
Photonic Crystal Slabs For Low Cost Biosensors
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Author : Yousef Nazirizadeh
language : en
Publisher: KIT Scientific Publishing
Release Date : 2014-10-16
Photonic Crystal Slabs For Low Cost Biosensors written by Yousef Nazirizadeh and has been published by KIT Scientific Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-16 with Science categories.
Biosensors are devices that utilize biological recognition elements to selectively detect and analyze specific biological and chemical analyte substances. In this work a technology platform for label-free optical biosensors based on surface-functionalized photonic crystal slabs is proposed. Using this technology platform, low-cost solutions for three biotechnical questions are presented.
Label Free Assays On Photonic Crystal Biosensor
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Author : Maria Fernanda Pineda
language : en
Publisher:
Release Date : 2008
Label Free Assays On Photonic Crystal Biosensor written by Maria Fernanda Pineda and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Development And Optimization Of A Label Free Optical Biosensor Based On Bloch Surface Waves Sustained By One Dimensional Photonic Crystals
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Author : Valeria Moi
language : en
Publisher:
Release Date : 2015
Development And Optimization Of A Label Free Optical Biosensor Based On Bloch Surface Waves Sustained By One Dimensional Photonic Crystals written by Valeria Moi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
Development Of A Two Dimensional Photonic Crystal Biosensing Platform
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Author : James E.. Baker
language : en
Publisher:
Release Date : 2015
Development Of A Two Dimensional Photonic Crystal Biosensing Platform written by James E.. Baker and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
"Early detection of illness is critical both for controlling the spread of disease and for administering successful treatment, but it requires rapid and sensitive diagnostic capabilities. Label-free sensing provides a significant advantage in achieving this goal because it eliminates the need for auxiliary reagent materials or sample preparation. Various refractive index (RI) based optical sensors have shown promise as sensitive, label-free platforms for pathogen detection. Sensing platforms using two-dimensional photonic bandgap crystal structures, in particular, have shown the potential to achieve single-pathogen detection capabilities. The work contained in this dissertation describes the design, fabrication, and characterization of two-dimensional photonic crystal (PhC) structures to be implemented as sensitive, label-free diagnostic sensors for the detection of viral pathogens at low, or even single, copy numbers. Photonic crystals are composite materials in which unique optical properties result from a structurally periodic arrangement of materials with differing refractive indices (RI). Two-dimensional slab-PhCs are one such implementation in which a sub-wavelength layer of high-RI dielectric material is perforated with low-RI holes. By carefully designing the geometry of the slab-PhC, resonant optical cavities that are extraordinarily sensitive to small changes in local RI can be engineered so that infiltration of pathogenic analyte within the PhC structure causes a shift in optical resonance. Reduced optical transmission at the frequency of resonance allows detection to be observed by monitoring the transmission spectrum of the PhC device. These sensor transducers are compatible with standard semiconductor fabrication procedures and with microfluidic channel integration, permitting implementation in a lab-on-a-chip style sensing tool. We have pursued two 2D waveguide-coupled slab-PhC sensor geometries in this regard: one implementing a "small-defect" alteration of a periodic structure array, and the other incorporating a "large-defect" lattice disruption. Both have been interfaced with microfluidic flow channels to perform real-time sensing measurements. Detection of antibody proteins and virus-sized particles was demonstrated with the developed sensing platforms, and single-particle detection capabilities were confirmed for the large-defect PhC sensor. Computational electromagnetic field simulations indicate that both virus capture and small-molecule binding can be simultaneously measured and discerned from one another using this large-defect design, thus enabling background-corrected, single-pathogen target detection"--Pages iii-iv.
Photonic Crystal Biosensor Integrated Microfluidic Networks For High Throughput Label Free Bio Detection Applications
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Author : Charles J. Choi
language : en
Publisher:
Release Date : 2007
Photonic Crystal Biosensor Integrated Microfluidic Networks For High Throughput Label Free Bio Detection Applications written by Charles J. Choi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Using Photonic Crystal Biosensors For Pharmaceutical Screening
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Author : Sherine George
language : en
Publisher:
Release Date : 2010
Using Photonic Crystal Biosensors For Pharmaceutical Screening written by Sherine George and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
The application of photonic crystal surfaces for label-free bioimaging and sensing is presented. A label-free biosensor assay is used to monitor the density of cell attachment to the sensor surface, and to quantify changes in attached cell density induced by the introduction of plant extracts. This image-based biosensor detection approach can effectively identify plant extracts with cytotoxic effects on cancer cells using only 500 cells per test. A library of sixty-one plant extract from Bangladesh is studied. We have also demonstrated that cell death in extracttreated cells occurs via apoptosis. Next, we take a closer look at the cytotoxicity of extracts from a single genus 0́3 the Amoora genus, on a panel of five different cancer cell lines. The effect of the extraction method used in preparing the extract sample on cell cytotoxicity is explored. An ongoing project involving the screening of plant protein samples from Pakistan is then presented. In further work, the description of the photonic crystal biosensor used for a protein macromolecule binding assay is presented. A high-throughput screen aimed at identifying small molecule disrupters of protein-DNA binding is designed and executed. Both the cell-based assay and the protein-DNA based assay described here find important applications in the modern drug discovery process.