Label Free Assays On Photonic Crystal Biosensor
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Label Free Assays On Photonic Crystal Biosensor
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Author : Maria Fernanda Pineda
language : en
Publisher:
Release Date : 2008
Label Free Assays On Photonic Crystal Biosensor written by Maria Fernanda Pineda and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with categories.
Photonic Crystal Slabs For Low Cost Biosensors
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Author : Yousef Nazirizadeh
language : en
Publisher: KIT Scientific Publishing
Release Date : 2014-10-16
Photonic Crystal Slabs For Low Cost Biosensors written by Yousef Nazirizadeh and has been published by KIT Scientific Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-16 with Science categories.
Biosensors are devices that utilize biological recognition elements to selectively detect and analyze specific biological and chemical analyte substances. In this work a technology platform for label-free optical biosensors based on surface-functionalized photonic crystal slabs is proposed. Using this technology platform, low-cost solutions for three biotechnical questions are presented.
Using Photonic Crystal Biosensors For Pharmaceutical Screening
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Author : Sherine George
language : en
Publisher:
Release Date : 2010
Using Photonic Crystal Biosensors For Pharmaceutical Screening written by Sherine George and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010 with categories.
The application of photonic crystal surfaces for label-free bioimaging and sensing is presented. A label-free biosensor assay is used to monitor the density of cell attachment to the sensor surface, and to quantify changes in attached cell density induced by the introduction of plant extracts. This image-based biosensor detection approach can effectively identify plant extracts with cytotoxic effects on cancer cells using only 500 cells per test. A library of sixty-one plant extract from Bangladesh is studied. We have also demonstrated that cell death in extracttreated cells occurs via apoptosis. Next, we take a closer look at the cytotoxicity of extracts from a single genus 0́3 the Amoora genus, on a panel of five different cancer cell lines. The effect of the extraction method used in preparing the extract sample on cell cytotoxicity is explored. An ongoing project involving the screening of plant protein samples from Pakistan is then presented. In further work, the description of the photonic crystal biosensor used for a protein macromolecule binding assay is presented. A high-throughput screen aimed at identifying small molecule disrupters of protein-DNA binding is designed and executed. Both the cell-based assay and the protein-DNA based assay described here find important applications in the modern drug discovery process.
Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms
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Author : Rashmi Sriram
language : en
Publisher:
Release Date : 2015
Advancing The Biosensing Technologies Of Two Optical Label Free Sensing Platforms written by Rashmi Sriram and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015 with categories.
"Optical label-free biosensors promise high-throughput, rapid, and sensitive detection of biomolecules. As such, these biodetection platforms have applications in clinical diagnostics, pharmaceutical industry, homeland security and in environmental monitoring. Motivated by these attributes, my thesis has focused on advancing the biosensing technology of two novel silicon-based optical label-free sensors: arrayed imaging reflectometry (AIR) and two-dimensional photonic crystal structures (2D PhC). The AIR biosensor is an interferometry-based technique that relies on the creation and destruction of a near-zero reflectivity condition in a carefully chosen thin film. To thoroughly quantify the biosensor detection signal, a correlation between the sensor response and the solution analyte concentration needs to be determined. In the first half of this thesis, a theoretical framework was developed to predict the AIR sensor response for a given solution analyte concentration. The developed model was subsequently validated using spectroscopic ellipsometry and surface plasmon resonance techniques in addition to AIR by utilizing two model probe-analyte pairs. It was established that the probe-analyte interaction on AIR substrate across two formats: macrospots and microarrays closely represented one-site and two-site Langmuir models, respectively. Two-dimensional photonic crystals are periodic dielectric structures with spatial periodicity in two dimensions. In the presence of a "defect" microcavity that disrupts the spatial periodicity, light of specific wavelength can be captured within the defect cavity. This manifests as a "dip" in the transmission spectrum of the crystal and is very sensitive to the local refractive index changes in the immediate vicinity of the defect cavity. The second half of this thesis investigates the utility of two designs of the 2D PhC: "small" and "large" point-defect microcavities coupled to a transmission waveguide for sensing latex particles in microfluidic environment. With the "small" defect design, detection of ~100 nm size latex particles in microfluidic environment was confirmed. This is the first study that demonstrates particle detection in the presence of a fluid cover using a 2D PhC sensor, a significant technological advancement for the incorporation of this sensor into lab-on-a-chip device. Using a "large" point-defect cavity design, real-time detection of latex particles of size ~280 nm was demonstrated. This to our knowledge is the first demonstration of detection of latex particles in real-time using 2D PhCs without the aid of any optical forces. This proof-of-principle demonstration of particle detection lays the foundation for the further development of these devices for the detection of a single biological particle, which when accomplished will have a huge impact in the field of clinical diagnostics"--Pages ix-x.
Study And Development Of Label Free Optical Biosensors For Biomedical Applications
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Author : Charles J. Choi
language : en
Publisher:
Release Date : 2011
Study And Development Of Label Free Optical Biosensors For Biomedical Applications written by Charles J. Choi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with categories.
For the majority of assays currently performed, fluorescent or colorimetric chemical labels are commonly attached to the molecules under study so that they may be readily visualized. The methods of using labels to track biomolecular binding events are very sensitive and effective, and are employed as standardized assay protocol across research labs worldwide. However, using labels induces experimental uncertainties due to the effect of the label on molecular conformation, active binding sites, or inability to find an appropriate label that functions equivalently for all molecules in an experiment. Therefore, the ability to perform highly sensitive biochemical detection without the use of fluorescent labels would further simplify assay protocols and would provide quantitative kinetic data, while removing experimental artifacts from fluorescent quenching, shelf-life, and background fluorescence phenomena. In view of the advantages mentioned above, the study and development of optical label-free sensor technologies have been undertaken here. In general, label-free photonic crystal (PC) biosensors and metal nanodome array surface-enhanced Raman scattering (SERS) substrates, both of which are fabricated by nanoreplica molding process, have been used as the method to attack the problem. Chapter 1 shows the work on PC label-free biosensor incorporated microfluidic network for bioassay performance enhancement and kinetic reaction rate constant determination. Chapter 2 describes the work on theoretical and experimental comparison of label-free biosensing in microplate, microfluidic, and spot-based affinity capture assays. Chapter 3 shows the work on integration of PC biosensor with actuate-to-open valve microfluidic chip for pL-volume combinatorial mixing and screening application. In Chapter 4, the development and characterization of SERS nanodome array is shown. Lastly, Chapter 5 describes SERS nanodome sensor incorporated tubing for point-of-care monitoring of intravenous drugs and metabolites.
Photonic Crystal Biosensor Integrated Microfluidic Networks For High Throughput Label Free Bio Detection Applications
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Author : Charles J. Choi
language : en
Publisher:
Release Date : 2007
Photonic Crystal Biosensor Integrated Microfluidic Networks For High Throughput Label Free Bio Detection Applications written by Charles J. Choi and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with categories.
Development Of Photonic Crystal Biosensor For Label Free Bioassays
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Author : Syed Hussain
language : en
Publisher:
Release Date : 2011
Development Of Photonic Crystal Biosensor For Label Free Bioassays written by Syed Hussain and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011 with Biological assay categories.
Tunable Photonic Crystal Biosensors For Portable Label Free Diagnostics
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Author : Meredith Marie Lee
language : en
Publisher:
Release Date : 2012
Tunable Photonic Crystal Biosensors For Portable Label Free Diagnostics written by Meredith Marie Lee and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012 with categories.
Although there is a pressing global need for widely-deployable disease detection and monitoring systems, today's options for biochemical analysis are often bulky, slow, expensive, and reliant on trained medical personnel. In contrast, the miniaturization and integration of devices based on arrays of sources, detectors, and active or passive biosensing surfaces provides a means to achieve handheld diagnostic capabilities with a "lab-on-a-chip" that would be vastly less expensive and fully automated. In this thesis, we design, fabricate, and characterize tunable biosensors with compact and low-cost Vertical Cavity Surface Emitting Lasers (VCSELs), integrated detectors, Peltier coolers, photonic crystal slab resonators, and polymer microfluidics. All of the components utilize scalable semiconductor and soft-lithography techniques to significantly reduce the required device footprint, simplify system assembly, and enable large-scale, economical manufacturing. The sensors operate in the visible to near-infrared 650-900 nm wavelength range for low absorption of water, hemoglobin, and other background elements found in tissue or aqueous samples. Moreover, the 670 nm label-free sensors we demonstrate are designed for compatibility with previously characterized monolithically integrated fluorimeters that capitalize on emerging deep-red fluorescent proteins and molecular probes approved for pre-clinical use by the Federal Drug Administration.
External Cavity Laser Label Free Biosensor
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Author :
language : en
Publisher:
Release Date : 2013
External Cavity Laser Label Free Biosensor written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Label Free And Multi Parametric Monitoring Of Cell Based Assays With Substrate Embedded Sensors
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Author : Maximilian Oberleitner
language : en
Publisher: Springer
Release Date : 2017-09-26
Label Free And Multi Parametric Monitoring Of Cell Based Assays With Substrate Embedded Sensors written by Maximilian Oberleitner and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-26 with Science categories.
This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.