Dna Isolation And Sequencing
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Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2004
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.
Dna Isolation And Sequencing
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Author : Bruce A. Roe
language : en
Publisher: Wiley-Blackwell
Release Date : 1996-07-25
Dna Isolation And Sequencing written by Bruce A. Roe and has been published by Wiley-Blackwell this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-07-25 with Medical categories.
This book provides immediate information on how to perform DNA sequencing and isolation techniques. It presents step-by-step instructions for 40 of the most commonly used methods in the field.
Basic Techniques In Molecular Biology
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Author : Stefan Surzycki
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Basic Techniques In Molecular Biology written by Stefan Surzycki and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Nanopore Sequencing An Introduction
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Author : Daniel Branton
language : en
Publisher: World Scientific
Release Date : 2019-04-05
Nanopore Sequencing An Introduction written by Daniel Branton and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-04-05 with Science categories.
This is an introductory text and laboratory manual to be used primarily in undergraduate courses. It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing. The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration. Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology. The book addresses this complaint by concretely demonstrating the range of topics — from electricity to biochemistry, protein structure, molecular engineering, and informatics — that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
Techniques In Molecular Systematics And Evolution
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Author : Rob DeSalle
language : en
Publisher: Springer Science & Business Media
Release Date : 2002-04-01
Techniques In Molecular Systematics And Evolution written by Rob DeSalle and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-01 with Science categories.
The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased exponentially over the last ten years. The need for more rapid and efficient methods of data acquisition and analysis is growing accordingly. This manual presents some of the most important techniques for data acquisition developed over the last years. The choice and justification of data analysis techniques is also an important and critical aspect of modern phylogenetic and evolutionary analysis and so a considerable part of this volume addresses this important subject. The book is mainly written for students and researchers from evolutionary biology in search for methods to acquire data, but also from molecular biology who might be looking for information on how data are analyzed in an evolutionary context. To aid the user, information on web-located sites is included wherever possible. Approaches that will push the amount of information which systematics will gather in the
Biotechnologies For Plant Mutation Breeding
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Author : Joanna Jankowicz-Cieslak
language : en
Publisher: Springer
Release Date : 2016-12-08
Biotechnologies For Plant Mutation Breeding written by Joanna Jankowicz-Cieslak and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-12-08 with Science categories.
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Protocols In Lichenology
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Author : Ilse Kranner
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Protocols In Lichenology written by Ilse Kranner and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.
As an intricate association between a fungus and one or more green algae or cyanobacteria, lichens are one of the most successful examples of symbiosis. These fascinating organisms survive extreme desiccation and temperatures. They are adapted to a great variety of habitats, from deserts to intertidal zones, from tropical rain forests to the peaks of the Himalayas and to circumpolar ecosystems. Lichens are extremely efficient accumulators of atmospherically deposited pollutants, and are therefore widely used to monitor environmental pollution. Their wide range of secondary products show pharmaceutically interesting fungicidal, antibacterial and antiviral properties. Lichens are extremely difficult to culture. This manual provides well-tested tissue culture protocols, protocols for studying lichen ultrastructure, (eco)physiology, primary and secondary compounds, and for using lichens as bioindicators.
The Barley Genome
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Author : Nils Stein
language : en
Publisher: Springer
Release Date : 2018-08-18
The Barley Genome written by Nils Stein and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-18 with Science categories.
This book presents an overview of the state-of-the-art in barley genome analysis, covering all aspects of sequencing the genome and translating this important information into new knowledge in basic and applied crop plant biology and new tools for research and crop improvement. Unlimited access to a high-quality reference sequence is removing one of the major constraints in basic and applied research. This book summarizes the advanced knowledge of the composition of the barley genome, its genes and the much larger non-coding part of the genome, and how this information facilitates studying the specific characteristics of barley. One of the oldest domesticated crops, barley is the small grain cereal species that is best adapted to the highest altitudes and latitudes, and it exhibits the greatest tolerance to most abiotic stresses. With comprehensive access to the genome sequence, barley’s importance as a genetic model in comparative studies on crop species like wheat, rye, oats and even rice is likely to increase.
Protocols In Advanced Genomics And Allied Techniques
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Author : Aruna Pal
language : en
Publisher: Springer Nature
Release Date : 2021-11-14
Protocols In Advanced Genomics And Allied Techniques written by Aruna Pal and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-14 with Science categories.
This laboratory manual includes the latest tools and techniques involved in genomic research. It starts with an introductory chapter on genomics and the various tools and applications involved. The initial chapters present protocols for basic techniques such as DNA isolation, electrophoresis, PCR, cDNA synthesis etc. The book then goes on to describe more advanced techniques such as next-generation sequencing, exome sequencing, use of RNAi, RNAseq, genome editing, single cell genomics etc. Each topic includes a brief description, information on the principles involved, materials & methods, protocol, and expected results, with diagrams and graphs. All protocols are presented in a very lucid and precise way, to make it easy for readers to follow and replicate them.
Sample Preparation Techniques For Soil Plant And Animal Samples
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Author : Miodrag Micic
language : en
Publisher: Humana
Release Date : 2018-03-31
Sample Preparation Techniques For Soil Plant And Animal Samples written by Miodrag Micic and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-31 with Science categories.
The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation.