Dna Repair Methods And Protocols
DOWNLOAD
Download Dna Repair Methods And Protocols PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Dna Repair Methods And Protocols book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Dna Repair
DOWNLOAD
Author : Lata Balakrishnan
language : en
Publisher:
Release Date : 2019
Dna Repair written by Lata Balakrishnan and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019 with DNA repair categories.
Base Excision Repair Pathway
DOWNLOAD
Author : Kishor K. Bhakat
language : en
Publisher: Springer Nature
Release Date : 2023-09-14
Base Excision Repair Pathway written by Kishor K. Bhakat and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-09-14 with Science categories.
This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.
Dna Damage Responses
DOWNLOAD
Author : Nima Mosammaparast
language : en
Publisher:
Release Date : 2022
Dna Damage Responses written by Nima Mosammaparast and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022 with DNA damage categories.
This volume provides detailed methods and key approaches used to mechanistically study DNA damage, as well as the factors involved in the damage response. Chapters guide readers through proteomics and biophysical approaches, analyzing protein function, quantifying DNA replication dynamics and nucleic acid base damage, as well as biochemical reconstitution of key pathways involved in DNA repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Damage Responses: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.
In Situ Detection Of Dna Damage
DOWNLOAD
Author : Vladimir V. Didenko
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
In Situ Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Medical categories.
Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.
Dna Repair Protocols
DOWNLOAD
Author : Lotte Bjergb K
language : en
Publisher: Humana Press
Release Date : 2016-05-01
Dna Repair Protocols written by Lotte Bjergb K and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.
In its 3rd edition, this Methods in Molecular Biology book covers the eukaryotic response to genomic insult including advanced protocols and standard techniques in the field of DNA repair. Offers expert guidance for DNA repair, recombination, and replication."
Dna Repair Protocols
DOWNLOAD
Author : Pat Vaughan
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-02
Dna Repair Protocols written by Pat Vaughan and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-02 with Science categories.
When setting out to decide on the content of DNA Repair Protocols: Prokaryotic Systems, I was conscious of the need to portray the vast array of pathways and enzymatic activities that are part of the discipline of DNA repair. In addition to the classical DNA repair activities, I wanted to convey the significant interest that has been generated in recent years in the use of the proteins and repair systems as research tools, much like the use of restriction enzymes over the last few decades. Therefore, in addition to chapters deta- ing protocols for investigating specific repair activities, I have included s- eral chapters in this book on the applied use of DNA repair proteins and systems. The many years of research on bacterial DNA repair systems have allowed us to really understand the majority of DNA repair pathways in bac- rial cells. Building on this knowledge, research has lead to major advances in understanding mammalian DNA repair and uncovered its links to human d- ease, such as DNA mismatch repair and colon cancer, nucleotide excision repair and xeroderma pigmentosum, DNA helicase function in Bloom’s s- drome, and so on. Such have been the advances that Science magazine iden- fied the collective DNA repair systems as its “Molecule of the Year” in 1994.
Fast Detection Of Dna Damage
DOWNLOAD
Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2017-07-14
Fast Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-14 with Medical categories.
This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
Dna Recombination
DOWNLOAD
Author : Hideo Tsubouchi
language : en
Publisher: Humana Press
Release Date : 2011-06-09
Dna Recombination written by Hideo Tsubouchi and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-09 with Medical categories.
Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube. Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.
Homologous Recombination
DOWNLOAD
Author : Andres Aguilera
language : en
Publisher: Humana
Release Date : 2020-10-17
Homologous Recombination written by Andres Aguilera and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-10-17 with Medical categories.
This volume explores homologous recombination’s (HR) essential role in meiotic and somatic cells. It discusses the analysis of different steps of the HR process from the genetic, molecular biology, and cell biology perspectives. Using a variety of model systems, chapters in this book cover topics such as the genome-wide mapping of DNA double-strand breaks (DSB); analysis of DNA-end resection and recombination intermediates by gel electrophoresis and southern blotting; cell-based monitoring of HR activity in meiotic and mitotic cells; in vitro analysis of branch migration, DNA strand exchange and Holliday junction resolution; or super resolution imaging of HR repair at collapsed replication forks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Homologous Recombination: Methods and Protocols is a valuable resource that uses both classical and more recent approaches to answer questions on the HR mechanism. It is a useful tool for scientists working on the field of genome integrity, as well as those working in cancer biology and DNA repair.
Dna Repair Protocols
DOWNLOAD
Author : Daryl S. Henderson
language : en
Publisher: Humana Press
Release Date : 1999-06-21
Dna Repair Protocols written by Daryl S. Henderson and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1999-06-21 with Science categories.