In Situ Detection Of Dna Damage

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In Situ Detection Of Dna Damage

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Author : Vladimir V. Didenko
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05

In Situ Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Medical categories.

Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.

Dna Damage Detection In Situ Ex Vivo And In Vivo

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Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2011-06-23

Dna Damage Detection In Situ Ex Vivo And In Vivo written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-23 with Medical categories.

Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.

Technologies For Detection Of Dna Damage And Mutations

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Author : G.P. Pfeifer
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11

Technologies For Detection Of Dna Damage And Mutations written by G.P. Pfeifer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.

Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity. Throughout the evolution oflife, complex DNA repair systems have developed in all living organisms to cope with this damage. Unrepaired DNA lesions can promote genetic alterations (mutations) that may be linked to an altered phenotype, and, if growth-controlling genes are involved, these mutations can lead to cell transformation and the development of malignant tumors. Proto oncogenes and tumor suppressor genes may be critical targets for DNA damaging agents. In a number of animal model systems, correlations between exposure to a carcinogen, tumor develop ment, and genetic changes in tumor DNA have been established. To understand mutagenesis processes in more detail at the molecular level, we need to know the type and frequency of DNA adducts within cells, their distribution along genes and specific DNA sequences, as well as the rates at which they are repaired. We also need to know what types of mutations are produced and which gene positions are most prone to mutagenesis. This book provides a collection of techniques that are useful in mutagenesis research. The book is divided into three parts. In Part I, methods for DNA damage and repair analysis are provided.

In Situ Detection Of Dna Damage

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Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2002-04-30

In Situ Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-04-30 with Medical categories.

Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.

Fast Detection Of Dna Damage

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Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2017-07-14

Fast Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-14 with Medical categories.

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

Eukaryotic Dna Damage Surveillance And Repair

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Author : Keith William Caldecott
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-05-31

Eukaryotic Dna Damage Surveillance And Repair written by Keith William Caldecott and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-31 with Science categories.

Eukaryotic DNA Damage Surveillance and Repair contains chapters from experts in the field of DNA damage detection, repair, and cell cycle control. The work reviews current understanding of how different types of DNA damage are detected and focuses on how these surveillance mechanisms are coupled to processes of DNA repair, cell cycle control, and apoptosis. The title will be of interest to undergraduate/postgraduate students and academics alike.

Molecular Biology Of The Cell

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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002

Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.

Dna Repair Protocols

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Author : Lotte Bjergb K
language : en
Publisher: Humana Press
Release Date : 2016-05-01

Dna Repair Protocols written by Lotte Bjergb K and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-05-01 with Medical categories.

In its 3rd edition, this Methods in Molecular Biology book covers the eukaryotic response to genomic insult including advanced protocols and standard techniques in the field of DNA repair. Offers expert guidance for DNA repair, recombination, and replication."

Postlabelling Methods For Detection Of Dna Adducts

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Author : D. H. Phillips
language : en
Publisher: Oxford University Press, USA
Release Date : 1993

Postlabelling Methods For Detection Of Dna Adducts written by D. H. Phillips and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Affinity labeling categories.

In experimental animal studies the quantitation of DNA adducts has usually required the use of highly radioactive chemical carcinogens. However a major breakthrough in detection methods occurred in the early 1980s with the development of the 32P-postlab

Reproductive Toxicology

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Author : Jesús del Mazo
language : en
Publisher: Springer Science & Business Media
Release Date : 1998-10-31

Reproductive Toxicology written by Jesús del Mazo and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-10-31 with Medical categories.

Reviews the current practices of analyzing germ and interacting cell in vitro culture systems to investigate toxicological damage on germ cells and reproductive systems. Focuses on methodology rather than findings, interpretations, or clinical applications. Covers advances in improving human risk estimation, new approaches to analyzing the exposure of germ cells to toxicological agents in vitro, molecular and developmental approaches to assessing reproductive toxicity, and comparing the assessment of animal risk in vivo and in vitro. Among specific topics are assays to measure estrogen and androgen agonists and antagonists, flow cytometry to evaluate testicular toxicity, current and future contributions of transgenic mice in analyzing germline toxicology, and a new assay to assess aneuploidy in human-hamster embryos. The 25 papers are from an October 1997 workshop in Granada, Spain. Annotation copyrighted by Book News, Inc., Portland, OR