Fast Detection Of Dna Damage
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Fast Detection Of Dna Damage
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Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2017-07-14
Fast Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-07-14 with Medical categories.
This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the “research trenches” they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.
In Situ Detection Of Dna Damage
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Author : Vladimir V. Didenko
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
In Situ Detection Of Dna Damage written by Vladimir V. Didenko and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Medical categories.
Detection and analysis of DNA damage is of critical importance in a variety of biological disciplines studying apoptosis, cell cycle and cell di- sion, carcinogenesis, tumor growth, embryogenesis and aging, neu- degenerative and heart diseases, anticancer drug development, environmental and radiobiological research, and others. Individual cells within the same tissue or in cell culture may vary in the extent of their DNA damage and, consequently, can display different re- tions to it. These differences between individual cells in the same cell popu- tion are detected using in situ approaches. In situ is a Latin term meaning “on site” or “in place.” It is used to denote the processes occurring or detected in their place of origin. In mole- lar and cell biology this usually refers to undisrupted mounted cells or tissue sections. In that meaning “in situ” is used as part of the terms “in situ PCR,” “in situ transcription,” “in situ hybridization,” “in situ end labeling,” and “in situ ligation.” Sometimes the “in situ” term is applied at the subcellular level to cells disrupted in the process of analysis, for example, in the detection of specific sequences in chromosomes using fluorescent in situ hybridization (FISH). Historically, the term was used primarily in methods dealing with nucleic acids.
Technologies For Detection Of Dna Damage And Mutations
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Author : G.P. Pfeifer
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Technologies For Detection Of Dna Damage And Mutations written by G.P. Pfeifer and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
Man-made carcinogens, natural genotoxic agents in the environment, as well as ionizing and ultraviolet radiation can damage DNA and are a constant threat to genome integrity. Throughout the evolution oflife, complex DNA repair systems have developed in all living organisms to cope with this damage. Unrepaired DNA lesions can promote genetic alterations (mutations) that may be linked to an altered phenotype, and, if growth-controlling genes are involved, these mutations can lead to cell transformation and the development of malignant tumors. Proto oncogenes and tumor suppressor genes may be critical targets for DNA damaging agents. In a number of animal model systems, correlations between exposure to a carcinogen, tumor develop ment, and genetic changes in tumor DNA have been established. To understand mutagenesis processes in more detail at the molecular level, we need to know the type and frequency of DNA adducts within cells, their distribution along genes and specific DNA sequences, as well as the rates at which they are repaired. We also need to know what types of mutations are produced and which gene positions are most prone to mutagenesis. This book provides a collection of techniques that are useful in mutagenesis research. The book is divided into three parts. In Part I, methods for DNA damage and repair analysis are provided.
Dna Damage Detection In Situ Ex Vivo And In Vivo
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Author : Vladimir V. Didenko
language : en
Publisher: Humana Press
Release Date : 2011-06-23
Dna Damage Detection In Situ Ex Vivo And In Vivo written by Vladimir V. Didenko and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-23 with Medical categories.
Recent advances in organic chemistry, fluorescent microscopy, and materials science have created an entirely new range of techniques and probes for imaging DNA damage in molecular and cellular biology. In DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols, expert researchers explore the latest advances in the area, covering both recent and established techniques to detect and quantify DNA damage at scales ranging from subcellular to the level of a whole live organism. Chapters present all major assays used in molecular and cellular biology for the labeling of DNA damage in situ, ex vivo, and in vivo. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, DNA Damage Detection In Situ, Ex Vivo, and In Vivo: Methods and Protocols is an essential handbook for novice and experienced researchers in a variety of fields, including molecular and cellular biology, experimental and clinical pathology, toxicology, radiobiology, oncology, embryology, experimental pharmacology, drug design, and environmental science.
Eukaryotic Dna Damage Surveillance And Repair
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Author : Keith William Caldecott
language : en
Publisher: Springer Science & Business Media
Release Date : 2004-05-31
Eukaryotic Dna Damage Surveillance And Repair written by Keith William Caldecott and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-05-31 with Science categories.
Eukaryotic DNA Damage Surveillance and Repair contains chapters from experts in the field of DNA damage detection, repair, and cell cycle control. The work reviews current understanding of how different types of DNA damage are detected and focuses on how these surveillance mechanisms are coupled to processes of DNA repair, cell cycle control, and apoptosis. The title will be of interest to undergraduate/postgraduate students and academics alike.
The Comet Assay In Toxicology
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Author : Alok Dhawan
language : en
Publisher: Royal Society of Chemistry
Release Date : 2016-10-07
The Comet Assay In Toxicology written by Alok Dhawan and has been published by Royal Society of Chemistry this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-07 with Medical categories.
Concerns about the adverse effects of chemicals present in the environment have created a need for better systems to assess their potential consequences on human health. One potential solution is the versatile and state-of-the-art Comet assay. Simple, sensitive, rapid and visual, this modern toxicological method allows quantitative and qualitative assessment of DNA damage in single cells. This unique reference is devoted exclusively to the Comet assay and addresses, in-depth, the different protocols, statistical analyses and applications being used worldwide. It also includes the guidelines recommended by the working group on Comet assay. The book is aimed at students as well as scientists in the area of molecular epidemiology and genetic toxicology.
Postlabelling Methods For Detection Of Dna Adducts
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Author : D. H. Phillips
language : en
Publisher: Oxford University Press, USA
Release Date : 1993
Postlabelling Methods For Detection Of Dna Adducts written by D. H. Phillips and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 1993 with Affinity labeling categories.
In experimental animal studies the quantitation of DNA adducts has usually required the use of highly radioactive chemical carcinogens. However a major breakthrough in detection methods occurred in the early 1980s with the development of the 32P-postlab
Molecular Biology Of The Cell
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Author : Bruce Alberts
language : en
Publisher:
Release Date : 2002
Molecular Biology Of The Cell written by Bruce Alberts and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002 with Cytology categories.
Protein Phosphorylation In Human Health
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Author : Cai Huang
language : en
Publisher: IntechOpen
Release Date : 2012-09-06
Protein Phosphorylation In Human Health written by Cai Huang and has been published by IntechOpen this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-09-06 with Medical categories.
15 chapters on protein phosphorylation and human health written by expert scientists. Covers most important research hot points, such as Akt, AMPK and mTOR. Bridges the basic protein phosphorylation pathways with human health and diseases. Detailed and comprehensive text with excellent figure illustration.
Chemical Carcinogenesis
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Author : Claudio Nicolini
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-03-09
Chemical Carcinogenesis written by Claudio Nicolini and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-03-09 with Medical categories.
During October 18-30, 1981, the second course of the International School of Pure and Applied Biostructure, a NATO Advanced Study Institute, was held at the Ettore Majorana Center for Scientific Culture in Erice, Italy, co-sponsored by the International Union Against Cancer, the Italian League Against Cancer, the Italian Ministry of Public Education, the Italian Ministry of Scientific and Technological Research, the North Atlantic Treaty Organization, the Italian National Research Council, the Sicilian Regional Government and two pharmaceutical Companies (Zambeletti and Farmitalia). The subject of the course was "Chemical Carcino genesis" with participants selected world-wide from 18 different countries. It is now eminently clear that.the bulk of human cancers are related to one of several types of environmental exposure. Of the environmental hazards, chemicals are among the best characterized carcinogens. However, how chemicals induce cancer is still poorly understood. Because of the magnitude of the problem and the ob vious need for a much more critical scientific analysis of the process by which cancer is induced (carcinogenesis), it was highly desirable to expose a greater number of scientists with varying background to some of the latest thinking in chemical carcino genesis. The course had this as its major objective and the re sulting book does reflect it.