E Coli Plasmid Vectors

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E Coli Plasmid Vectors

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Author : Nicola Casali
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03

E Coli Plasmid Vectors written by Nicola Casali and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.

A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.

Vectors

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Author : Raymond L. Rodriguez
language : en
Publisher: Butterworth-Heinemann
Release Date : 1988

Vectors written by Raymond L. Rodriguez and has been published by Butterworth-Heinemann this book supported file pdf, txt, epub, kindle and other format this book has been release on 1988 with Science categories.

Vectors: A Survey of Molecular Cloning Vectors and Their Uses focuses on the functions of molecular cloning vectors.

Techniques In Molecular Biology

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Author : J.M. Walker
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Techniques In Molecular Biology written by J.M. Walker and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

The last few years have seen the rapid development of new methodology in the field of molecular biology. New techniques have been regularly introduced and the sensitivity of older techniques greatly improved upon. Developments in the field of genetic engineering in particular have contributed a wide range of new techniques. The purpose of this book therefore is to introduce the reader to a selection of the more advanced analytical and preparative techniques which the editors consider to be frequently used by research workers in the field of molecular biology. In choosing techniques for this book we have obviously had to be selective, and for the sake of brevity a knowledge of certain basic biochemical techniques and terminology has been assumed. However, since many areas of molecular biology are developing at a formidable rate and constantly generating new terminology, a glossary of terms has been included. The techniques chosen for this book are essentially based on those used in a series of workshops on 'techniques in molecular biology' that have been held at The Hatfield Polytechnic in recent years. In choosing these chapters we have taken into account many useful suggestions and observations made by participants at these workshops. Each chapter aims to describe both the theory and relevant practical details for a given technique, and to identify both the potential and limitations of the technique. Each chapter is written by authors who regularly use the technique in their own laboratories.

Gene Cloning And Dna Analysis

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Author : T. A. Brown
language : en
Publisher: John Wiley & Sons
Release Date : 2013-04-25

Gene Cloning And Dna Analysis written by T. A. Brown and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-25 with Science categories.

Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "... the book content is elegantly illustrated and well organized in clear-cut chapters and subsections... there is a Further Reading section after each chapter that contains several key references... What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)

Molecular Biology Pathogenicity And Ecology Of Bacterial Plasmids

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Author : Stuart B. Levy
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Molecular Biology Pathogenicity And Ecology Of Bacterial Plasmids written by Stuart B. Levy and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.

This book resulted from presentations at an international conference on bacterial p1asmids held January 5-9, 1981 in Santo Domingo, Dominican Republic. This was the first meeting of its kind in the Southern Hemisphere. The meeting place was selected for its relaxed and comfortable climate, conducive to interactions among participants. More importantly the locale facilitated the participation of nearby Latin American clinical and research scientists who deal directly with the health manifestations of pathogenic p1asmids. Diseases and socio-economic practices of developing countries exist in the Dominican Republic whose scientific community could directly benefit from having the meeting there. The book includes the talks as well as extended abstracts of poster presentations from the meeting. This combination, which provides readers with reviews as well as recent findings, captures the full scientific exchange which took place during the 5-day meeting. As one indication of pathogenicity related to p1asmids, the conferees were surveyed for gastro-intestina1 problems during and after their stay in the Dominican Republic. The results are summarized at the end of this book.

Molecular Biology Of The Cell

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Author :
language : en
Publisher:
Release Date : 2004

Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.

Genetic Techniques For Biological Research

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Author : Corinne A. Michels
language : en
Publisher: John Wiley & Sons
Release Date : 2002-06-10

Genetic Techniques For Biological Research written by Corinne A. Michels and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-06-10 with Science categories.

"Genetic Techniques for Biological Research" ist ein Lehrbuch für fortgeschrittene Studenten und Doktoranden der Genetik, Molekularbiologie und Zellbiologie. Es basiert auf Fallstudien zur Hefe Saccharomyces als genetischem Modellorganismus, an dem anschaulich Theorie und Praxis der molekulargenetischen Analyse demonstriert wird. Darüber hinaus bietet es dem Leser umfassende Informationen, damit er diesen Ansatz in seine eigenen Forschungsprojekte einbauen kann. Autorin Corinne Michels - eine Expertin auf dem Gebiet der Hefegenetik und Molekularbiologie - erklärt hier genau, wie man praktische genetische Studien mit Hilfe von durchgearbeiteten Beispielen kritisch bewertet. Auf diese Weise soll der Leser die Fähigkeit zu kritischem Denken entwickeln, um das Material in eigenen Forschungsarbeiten anwenden zu können. Ein idealer Studienbegleiter zu Theorie und Praxis der molekulargenetischen Analyse!

Vaccine Development And Manufacturing

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Author : Emily P. Wen
language : en
Publisher: John Wiley & Sons
Release Date : 2014-10-06

Vaccine Development And Manufacturing written by Emily P. Wen and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-06 with Technology & Engineering categories.

Vaccine Manufacturing and Production is an invaluable reference on how to produce a vaccine - from beginning to end - addressing all classes of vaccines from a processing, production, and regulatory viewpoint. It will provide comprehensive information on the various fields involved in the production of vaccines, from fermentation, purification, formulation, to regulatory filing and facility designs. In recent years, there have been tremendous advances in all aspects of vaccine manufacturing. Improved technology and growth media have been developed for the production of cell culture with high cell density or fermentation. Vaccine Manufacturing and Production will serve as a reference on all aspects of vaccine production by providing an in-depth description of the available technologies for making different types of vaccines and the current thinking in facility designs and supply issues. This book will provide insight to the issues scientists face when producing a vaccine, the steps that are involved, and will serve as a reference tool regarding state-of-the-art vaccine manufacturing technologies and facility set-up. Highlights include: Comprehensive coverage of vaccine production : from a process point of view- fermentation to purification to formulation developments; from a production point of view - from facility design to manufacturing; and from a regulatory point of view - requirements from government agencies Authors from different major pharmaceutical and biotechnology companies Describes the challenges and issues involved in vaccine production and manufacturing of the different classes of vaccines, an area not covered by other books currently on the market

Textbook On Cloning Expression And Purification Of Recombinant Proteins

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Author : Kakoli Bose
language : en
Publisher: Springer Nature
Release Date : 2022-01-25

Textbook On Cloning Expression And Purification Of Recombinant Proteins written by Kakoli Bose and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-25 with Medical categories.

This book is immensely useful for graduate students as well as researchers to understand the basics of molecular biology and Recombinant DNA Technology. It provides a comprehensive overview of different approaches for the synthesis of recombinant proteins from E. coli including their cloning, expression and purification. Recent advances in genomics, proteomics, and bioinformatics have facilitated the use of Recombinant DNA Technology for evaluating the biophysical and biochemical properties of various proteins. The book starts with an introductory chapter on gene cloning, protein expression and purification and its implication in current research and commercial applications. Each chapter provides a lucid set of principles, tools and techniques for both students and instructors. The protocols described have been aptly exemplified, and troubleshooting techniques have been included to aid better understanding. Moreover, the set of questions at the end of each chapter have been particularly formulated to help effective learning.