Electron Crystallography Of Biological Macromolecules
DOWNLOAD
Download Electron Crystallography Of Biological Macromolecules PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Electron Crystallography Of Biological Macromolecules book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Electron Crystallography Of Biological Macromolecules
DOWNLOAD
Author : Robert M. Glaeser
language : en
Publisher: Oxford University Press, USA
Release Date : 2007
Electron Crystallography Of Biological Macromolecules written by Robert M. Glaeser and has been published by Oxford University Press, USA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007 with Science categories.
This book provides a complete introduction to all major topics needed in order to use electron microscopy as a research tool in structural biology. Written by a group of 5 well-known pioneers of the field of electron cryo-microscopy of biological macromolecules, this book offers a depth of knowledge and expertise that could only be replicated from the primary literature with great difficulty.
Three Dimensional Electron Microscopy Of Macromolecular Assemblies
DOWNLOAD
Author : Joachim Frank
language : en
Publisher: Oxford University Press
Release Date : 2006-02-02
Three Dimensional Electron Microscopy Of Macromolecular Assemblies written by Joachim Frank and has been published by Oxford University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-02-02 with Science categories.
Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.
Macromolecular Crystallography
DOWNLOAD
Author : Maria Armenia Carrondo
language : en
Publisher: Springer
Release Date : 2011-12-01
Macromolecular Crystallography written by Maria Armenia Carrondo and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-01 with Science categories.
This volume is a collection of the contributions presented at the 42nd Erice Crystallographic Course whose main objective was to train the younger generation on advanced methods and techniques for examining structural and dynamic aspects of biological macromolecules. The papers review the techniques used to study protein assemblies and their dynamics, including X-ray diffraction and scattering, electron cryo-electron microscopy, electro nanospray mass spectrometry, NMR, protein docking and molecular dynamics. A key theme throughout the book is the dependence of modern structural science on multiple experimental and computational techniques, and it is the development of these techniques and their integration that will take us forward in the future.
Structural Biology Using Electrons And X Rays
DOWNLOAD
Author : Michael F Moody
language : en
Publisher: Academic Press
Release Date : 2011-03-03
Structural Biology Using Electrons And X Rays written by Michael F Moody and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-03 with Science categories.
Structural Biology Using Electrons and X-Rays discusses the diffraction and image-based methods used for the determination of complex biological macromolecules. The book focuses on the Fourier transform theory, which is a mathematical function that is computed to transform signals between time and frequency domain. Composed of five parts, the book examines the development of nuclear magnetic resonance (NMR), which allows the calculation of the images of a certain protein. Parts 1 to 4 provide the basic information and the applications of Fourier transforms, as well as the different methods used for image processing using X-ray crystallography and the analysis of electron micrographs. Part 5 focuses entirely on the mathematical aspect of Fourier transforms. In addition, the book examines detailed structural analyses of a specimen’s symmetry (i.e., crystals, helices, polyhedral viruses and asymmetrical particles). This book is intended for the biologist or biochemist who is interested in different methods and techniques for calculating the images of proteins using nuclear magnetic resonance (NMR). It is also suitable for readers without a background in physical chemistry or mathematics. Emphasis on common principles underlying all diffraction-based methods Thorough grounding in theory requires understanding of only simple algebra Visual representations and explanations of challenging content Mathematical detail offered in short-course form to parallel the text
International Tables For Crystallography Crystallography Of Biological Macromolecules
DOWNLOAD
Author : Eddy Arnold
language : en
Publisher: Wiley
Release Date : 2012-03-05
International Tables For Crystallography Crystallography Of Biological Macromolecules written by Eddy Arnold and has been published by Wiley this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-03-05 with Science categories.
International Tables for Crystallography Volume F is an expert guide to macromolecular crystallography for the structural biologist. It was commissioned by the International Union of Crystallography in recognition of the extraordinary contributions that knowledge of macromolecular structure has made, and will make, to the analysis of biological systems, from enzyme catalysis to the workings of a whole cell. The volume covers all stages of a crystallographic analysis from the preparation of recombinant proteins, through crystallization, diffraction data collection, phase determination, structure validation and structure analysis. Although the volume is written for experienced scientists, it is recognized that the reader is more likely to be a biologist interested in structure than a classical crystallographer interested in biology. Thus, there are chapters on the fundamentals, history and current perspectives of macromolecular crystallography, as well as on useful programs and databases such as the Protein Data Bank. Each chapter is written by one or more internationally recognized experts. This second edition features 19 new articles and many articles from the first edition have been revised. The new articles cover topics such as standard definitions for quality indicators, expression of membrane proteins, protein engineering, high-throughput crystallography, radiation damage, merohedral twinning, low-resolution ab initio phasing, robotic crystal loading, whole-cell X-ray diffraction imaging and halogen interactions in biological crystal structures. There are also new articles on relevant software, including software for electron microscopy. These enhancements will ensure that Volume F continues to be a key reference for macromolecular crystallographers and structural biologists. More information on the series can be found at: http://it.iucr.org
Single Particle Cryo Electron Microscopy
DOWNLOAD
Author : Joachim Frank
language : en
Publisher: World Scientific Publishing Company
Release Date : 2017-12-31
Single Particle Cryo Electron Microscopy written by Joachim Frank and has been published by World Scientific Publishing Company this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-12-31 with Electron microscopy categories.
The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as important results obtained by this technique in the study of biological macromolecules and their interactions. Importantly, neither symmetries nor ordered arrangements (as in two-dimensional crystals, helical assemblies, icosahedral viruses) are required. Although the biological applications are mainly in the area of ribosome structure and function, the elucidation of membrane channel structures and their activation and gating mechanisms are represented, as well. The book is introduced by a commentary that explains the original development of concepts, describes the contributions of the author's colleagues and students, and shows how challenges were overcome as the technique matured. Along the way, the ribosome served as an example for a macromolecule with intricate structure and conformational dynamics that pose challenges for three-dimensional visualization. Toward the end of the book -- bringing us to the present time -- molecular structures with near-atomic resolution are presented, and a novel type of computational analysis, manifold embedding, is introduced. Single-particle cryo-EM is currently revolutionizing structural biology, presenting a powerful alternative to X-ray crystallography as a means to solve the structure of biological macromolecules. The book presents in one place a number of articles containing key advances in mathematical and computational methods leading up to the present time. Secondly, the development of the technique over the years is reflected by ever-expanding discoveries in the field of ribosome structure and function. Thirdly, as all histories of ideas, the history of concepts pertaining to this new method of visualization is fascinating all in itself.
Electron Tomography
DOWNLOAD
Author : Joachim Frank
language : en
Publisher:
Release Date : 2014-01-15
Electron Tomography written by Joachim Frank and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-01-15 with categories.
Structural Study Of Heterogeneous Biological Samples By Cryoelectron Microscopy And Image Processing
DOWNLOAD
Author : H.E. White
language : en
Publisher: Createspace Independent Publishing Platform
Release Date : 2017-04-21
Structural Study Of Heterogeneous Biological Samples By Cryoelectron Microscopy And Image Processing written by H.E. White and has been published by Createspace Independent Publishing Platform this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-21 with categories.
In living organisms, biological macromolecules are intrinsically flexible and naturally exist in multiple conformations. Modern electron microscopy, especially at liquid nitrogen temperatures (cryo-EM), is able to visualise biocomplexes in nearly native conditions and in multiple conformational states. The advances made during the last decade in electronic technology and software development have led to the revelation of structural variations in complexes and also improved the resolution of EM structures. Nowadays, structural studies based on single particle analysis (SPA) suggests several approaches for the separation of different conformational states and therefore disclosure of the mechanisms for functioning of complexes. The task of resolving different states requires the examination of large datasets, sophisticated programs, and significant computing power. Some methods are based on analysis of two-dimensional images, while others are based on three-dimensional studies. In this review, we describe the basic principles implemented in the various techniques that are currently used in the analysis of structural conformations and provide some examples of successful applications of these methods in structural studies of biologically significant complexes.
X Ray Crystallography Of Biomacromolecules
DOWNLOAD
Author : Albrecht Messerschmidt
language : en
Publisher: John Wiley & Sons
Release Date : 2007-02-27
X Ray Crystallography Of Biomacromolecules written by Albrecht Messerschmidt and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-02-27 with Science categories.
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from this extremely clearly written book. Part A covers the theoretical basis and such experimental techniques as principles of x-ray diffraction, solutions for the phase problem and time-resolved x-ray crystallography. Part B includes case studies for different kinds of x-ray crystal structure determination, such as the MIRAS and MAD techniques, molecular replacement, and the difference Fourier technique.
Molecular Machines In Biology
DOWNLOAD
Author : Joachim Frank
language : en
Publisher: Cambridge University Press
Release Date : 2011-12-19
Molecular Machines In Biology written by Joachim Frank and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-12-19 with Science categories.
The concept of molecular machines in biology has transformed the medical field in a profound way. Many essential processes that occur in the cell, including transcription, translation, protein folding and protein degradation, are all carried out by molecular machines. This volume focuses on important molecular machines whose architecture is known and whose functional principles have been established by tools of biophysical imaging (X-ray crystallography and cryo-electron microscopy) and fluorescence probing (single-molecule FRET). This edited volume includes contributions from prominent scientists and researchers who understand and have explored the structure and functions of these machines. This book is essential for students and professionals in the medical field who want to learn more about molecular machines.