Electron Microscopy Of Model Systems

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Electron Microscopy Of Model Systems

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Author :
language : en
Publisher: Academic Press
Release Date : 2010-09-24

Electron Microscopy Of Model Systems written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-09-24 with Science categories.

The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. - Covers the preparation and analysis of model systems for biological electron microscopy - Includes the most popular systems but also organisms that are less frequently used in cell biology - Presents the currently most important methods for the preparation of biological specimens - First compendium covering the various aspects of sample preparation of very diverse biological systems

Cellular Electron Microscopy

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Author : J. Richard McIntosh
language : en
Publisher: Elsevier
Release Date : 2011-09-02

Cellular Electron Microscopy written by J. Richard McIntosh and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-02 with Science categories.

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. Cellular Electron Microscopy will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems. - Updates the numerous technological innovations that have improved the capabilities of electron microscopy - Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging - Chapters include a balance of "how to", "so what" and "where next", providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

Biological Electron Microscopy

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Author : Michael J. Dykstra
language : en
Publisher: Springer Science & Business Media
Release Date : 2003-12-31

Biological Electron Microscopy written by Michael J. Dykstra and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-12-31 with Science categories.

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Model Systems To Study The Mechanisms Of Neural Development And Disease

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Author : Parthiv Haldipur
language : en
Publisher: Frontiers Media SA
Release Date :

Model Systems To Study The Mechanisms Of Neural Development And Disease written by Parthiv Haldipur and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on with Science categories.

Scanning Electron Microscopy In Biology

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Author : R.G. Kessel
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Scanning Electron Microscopy In Biology written by R.G. Kessel and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

In the continuing quest to explore structure and to relate struc tural organization to functional significance, the scientist has developed a vast array of microscopes. The scanning electron microscope (SEM) represents a recent and important advance in the development of useful tools for investigating the structural organization of matter. Recent progress in both technology and methodology has resulted in numerous biological publications in which the SEM has been utilized exclusively or in connection with other types of microscopes to reveal surface as well as intracellular details in plant and animal tissues and organs. Because of the resolution and depth of focus presented in the SEM photograph when compared, for example, with that in the light microscope photographs, images recorded with the SEM have widely circulated in newspapers, periodicals and scientific journals in recent times. Considering the utility and present status of scanning electron microscopy, it seemed to us to be a particularly appropriate time to assemble a text-atlas dealing with biological applications of scanning electron microscopy so that such information might be presented to the student and to others not yet familiar with its capabilities in teaching and research. The major goal of this book, therefore, has been to assemble material that would be useful to those students beginning their study of botany or zoo logy, as well as to beginning medical students and students in advanced biology courses.

Correlative Light And Electron Microscopy

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Author :
language : en
Publisher: Academic Press
Release Date : 2012-10-23

Correlative Light And Electron Microscopy written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-23 with Science categories.

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. - Covers many areas of CLEM by the best international scientists in the field - Includes a brief history and overview on CLEM methods

Bioenergetics And Thermodynamics Model Systems

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Author : A. Braibanti
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06

Bioenergetics And Thermodynamics Model Systems written by A. Braibanti and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Science categories.

Proceedings of the NATO Advanced Study Institute, Tabiano, Parma, Italy, May 21-June 1, 1979

Electron Microscopy Based Tools For Imaging Cellular Circuits And Organisms

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Author : Yoshiyuki Kubota
language : en
Publisher: Frontiers Media SA
Release Date : 2019-12-30

Electron Microscopy Based Tools For Imaging Cellular Circuits And Organisms written by Yoshiyuki Kubota and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-12-30 with categories.

Organoids As Model Systems For Human Development Disease And Clinical Applications

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Author : Eumorphia Remboutsika
language : en
Publisher: Frontiers Media SA
Release Date : 2021-11-23

Organoids As Model Systems For Human Development Disease And Clinical Applications written by Eumorphia Remboutsika and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-23 with Science categories.

Electron Microscopy In Heterogeneous Catalysis

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Author : P.L Gai
language : en
Publisher: CRC Press
Release Date : 2003-01-14

Electron Microscopy In Heterogeneous Catalysis written by P.L Gai and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-01-14 with Science categories.

Catalysis is one of the most important technologies in the industrial world, controlling more than 90% of industrial chemical processes and essential for large-scale production of plastics and fuel. Exploring the most common type of catalysis used in industry, Electron Microscopy in Heterogeneous Catalysis provides a coherent account of heterogeneo