Electroporation And Sonoporation In Developmental Biology
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Electroporation And Sonoporation In Developmental Biology
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Author : Harukazu Nakamura
language : en
Publisher: Springer Science & Business Media
Release Date : 2009-01-31
Electroporation And Sonoporation In Developmental Biology written by Harukazu Nakamura and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-01-31 with Science categories.
In ovo electroporation is an epoch-making achievement in the study of developmental biology. With this method, experiments can be carried out in gain and loss of function in desired tissue at any desired stage in chick embryos. Introduction of a tetracycline-regulated gene expression system and a transposon system has further extended the potential of the method, making it possible to obtain long-term expression and to turn on and off a gene of interest. It is now applied to mice, aquatic animals, and even to plants for the study of developmental biology and for other purposes. In this book, the application of electroporation in many embryonic tissues and organs is introduced, with some chapters that deal with gene transfer in adults. Sonoporation, another useful tool, using ultrasonic waves instead of electric currents, for gene transfer to mesenchymal tissues is also introduced.
Animal Cell Electroporation And Electrofusion Protocols
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Author : Jac A. Nickoloff
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Animal Cell Electroporation And Electrofusion Protocols written by Jac A. Nickoloff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
A Device For Performing Sonoporation On Adherent Cell Cultures
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Author : Jonathan Lawrence Kivinen
language : en
Publisher:
Release Date : 2014
A Device For Performing Sonoporation On Adherent Cell Cultures written by Jonathan Lawrence Kivinen and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014 with Cells categories.
"Sonoporation, or cellular sonication, is the use of sound (typically ultrasonic frequencies) for modifying the permeability of the cell plasma membrane. This technique is usually used in molecular biology and non-viral gene therapy in order to allow uptake of large molecules such as DNA into the cell, in a cell disruption process called transfection or transformation. Sonoporation employs the acoustic cavitation of microbubbles to enhance delivery of these large molecules. The bioactivity of this technique is similar to, and in some cases found superior to, electroporation. Extended exposure to low-frequency (
Handbook Of Electroporation
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Author : Damijan Miklavčič
language : en
Publisher: Springer
Release Date : 2017-09-14
Handbook Of Electroporation written by Damijan Miklavčič and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-09-14 with Technology & Engineering categories.
This major reference work is a one-shot knowledge base on electroporation and the use of pulsed electric fields of high intensity and their use in biology, medicine, biotechnology, and food and environmental technologies. The Handbook offers a widespread and well-structured compilation of 156 chapters ranging from the foundations to applications in industry and hospital. It is edited and written by most prominent researchers in the field. With regular updates and growing in its volume it is suitable for academic readers and researchers regardless of their disciplinary expertise, and will also be accessible to students and serious general readers. The Handbook's 276 authors have established scholarly credentials and come from a wide range of disciplines. This is crucially important in a highly interdisciplinary field of electroporation and the use of pulsed electric fields of high intensity and its applications in different fields from medicine, biology, food processing, agriculture, process engineering, energy and environment. An Editorial Board of distinguished scholars from across the world has selected and reviewed the various chapters to ensure the highest quality of this Handbook. The book was edited by an international team of Section Editors: P. Thomas Vernier, Boris Rubinsky, Juergen Kolb, Damijan Miklavcic, Marie-Pierre Rols, Javier Raso, Richard Heller, Gregor Serša, Dietrich Knorr, and Eugene Vorobiev.
Septins
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Author :
language : en
Publisher: Academic Press
Release Date : 2016-07-26
Septins written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-07-26 with Science categories.
Septins provides established septin and molecular and developmental biologists and researchers new to the field with proven, state-of-art techniques and relevant historical background and theory to aid efficient design and effective implementation of experimental methodologies. Topics include the purification of septin proteins from diverse systems, their visualization in live cells, and their analysis by a variety of cutting-edge microscopy approaches. Provides the latest information on septins Includes both established and new technologies Brings together specialists from the field who contribute their expertise
Anticancer Genes
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Author : Stefan Grimm
language : en
Publisher: Springer
Release Date : 2014-07-08
Anticancer Genes written by Stefan Grimm and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-07-08 with Science categories.
This book discusses the emergence of a new class of genes with a specific anticancer activity. These genes, recently defined as “Anticancer Genes”, are reviewed in individual chapters on their mode of action, the specific cell death signals they induce, and the status of attempts to translate them into clinical application. Anticancer Genes provides an overview of this nascent field, its genesis, current state, and prospect. It discusses how Anticancer Genes might lead to the identification of a repertoire of signaling pathways directed against cellular alterations that are specific for tumor cells. With contributions from experts worldwide, Anticancer Genes is an essential guide to this dynamic topic for researchers and students in cancer research, molecular medicine, pharmacology and toxicology and genetics as well as clinicians and clinical researchers interested in the therapeutic potential of this exciting new field.
Handbook Of Seafood Quality Safety And Health Applications
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Author : Cesarettin Alasalvar
language : en
Publisher: John Wiley & Sons
Release Date : 2011-06-15
Handbook Of Seafood Quality Safety And Health Applications written by Cesarettin Alasalvar and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-15 with Technology & Engineering categories.
The global market for seafood products continues to increase year by year. Food safety considerations are as crucial as ever in this sector, and higher standards of quality are demanded even as products are shipped greater distances around the world. The current global focus on the connection between diet and health drives growth in the industry and offers commercial opportunities on a number of fronts. There is great interest in the beneficial effects of marine functional compounds such as omega-3 polyunsaturated fatty acids. Seafoods are well-known as low calorie foods, and research continues into the nutritional effects on, for example, obesity and heart disease. In addition, by-products of marine food processing can be used in nutraceutical applications. This book is a resource for those interested in the latest advances in the science and technology of seafood quality and safety as well as new developments in the nutritional effects and applications of marine foods. It includes chapters on the practical evaluation of seafood quality; novel approaches in preservation techniques; flavour chemistry and analysis; textural quality and measurement; packaging; the control of food-borne pathogens and seafood toxins. New research on the health-related aspects of marine food intake are covered, as well as the use of seafoods as sources of bioactives and nutraceuticals. The book is directed at scientists and technologists in academia, government laboratories and the seafood industries, including quality managers, processors and sensory scientists.
Nutraceutical Delivery Systems
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Author : Pankaj V. Dangre
language : en
Publisher: CRC Press
Release Date : 2022-08-18
Nutraceutical Delivery Systems written by Pankaj V. Dangre and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-08-18 with Medical categories.
This book highlights recent innovative work in nutraceutical delivery systems, focusing on strategies and approaches for delivering maximum health benefits from foods. It presents recent research-oriented work from diverse global perspectives on isolation techniques for nutraceutical components, phytosomes, liposomes, solid dispersions, micelles, self-emulsifying drug delivery systems, microemulsions, solid lipid nanoparticles, polyelectrolyte complexes, oral delivery, polymeric nanoparticles, and more. The book begins with an overview of recent facts and diverse perspectives on the use of nutraceuticals in medicine and proceeds to discuss recent techniques in isolation of nutraceuticals from plants and in solubility enhancement. It looks at innovations and advances in nanoparticles-based nutraceutical delivery, such as in solid lipid nanoparticles (SLNs), fabrication methods of therapeutic nanoparticles, and polymeric nanoparticles-based nutraceutical delivery system. It also discusses vesicular delivery systems and biphasic systems for nutraceutical applications. The book also looks at the challenges in oral delivery and the latest taste-masking techniques.
Avian Embryology
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-09-21
Avian Embryology written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-09-21 with Science categories.
This revised edition will continue to serve as the most complete and up-to-date guide to the use of the avian embryo in studies of vertebrate development. It will include new approaches to analysis of the chick genome, gene knock-out studies using RNA interference, morpholinos, and other cutting edge techniques. As with the original edition, emphasis has been placed on providing practical guidance, highlighting potentials and pitfalls of all key cell biological and embryological techniques. *fully revised second edition *organized into basic and advanced Methods *new section on Functional Genomics
Biomass Valorization
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Author : Davide Ravelli
language : en
Publisher: John Wiley & Sons
Release Date : 2021-05-24
Biomass Valorization written by Davide Ravelli and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-05-24 with Science categories.
Explore the potential of biomass-based chemicals with this comprehensive new reference from leading voices in the field With the depletion of fossil raw materials a readily ascertainable inevitability, the exploitation of biomass-based renewable derivatives becomes ever more practical and realistic. In Biomass Valorization: Sustainable Methods for the Production of Chemicals, accomplished researchers and authors Davide Ravelli and Chiara Samori deliver a thorough compilation of state-of-the-art techniques and most advanced strategies used to convert biomass into useful building blocks and commodity chemicals. Each chapter in this collection of insightful papers begins by detailing the core components of the described technology, along with a fulsome description of its advantages and limitations, before moving on to a discussion of recent advancements in the field. The discussions are grouped by the processed biomass, such as terrestrial biomass, aquatic biomass, and biomass-deriving waste. Readers will also benefit from the inclusion of: A thorough introduction to the role of biomass in the production of chemicals An exploration of biomass processing via acid, base and metal catalysis, as well as biocatalysis A practical discussion of biomass processing via pyrolysis and thermochemical-biological hybrid processes A concise treatment of biomass processing assisted by ultrasound and via electrochemical, photochemical and mechanochemical means Perfect for chemical engineers, catalytic chemists, biotechnologists, and polymer chemists, Biomass Valorization: Sustainable Methods for the Production of Chemicals will also earn a place in the libraries of environmental chemists and professionals working with organometallics and natural products chemists.