Enhanced Biological Phosphorus Removal At High Temperature Using Different Carbon Sources Key Microorganisms And Processes
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Enhanced Biological Phosphorus Removal At High Temperature Using Different Carbon Sources Key Microorganisms And Processes
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Author : Rogelio Ernesto Zuniga Montanez
language : en
Publisher:
Release Date : 2020
Enhanced Biological Phosphorus Removal At High Temperature Using Different Carbon Sources Key Microorganisms And Processes written by Rogelio Ernesto Zuniga Montanez and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020 with categories.
Municipal wastewater contains a number of constituents that can have detrimental effects if discharged to receiving water bodies. Phosphorus (P) is of specific interest as a limiting nutrient in aquatic ecosystems that can cause eutrophication. In enhanced biological phosphorus removal (EBPR), polyphosphate accumulating organisms (PAOs) store excess P intracellularly. To achieve this accumulation, the organisms are exposed consecutively to anaerobic and either aerobic or anoxic conditions. During the anaerobic phase, PAOs consume and store organic carbon with P release, followed by the aerobic/anoxic phase during which the stored carbon is oxidized and P is taken up and stored as polyphosphate. PAOs are not the only bacteria that can thrive under these cyclic conditions and they face competition from glycogen accumulating organisms (GAOs). The latter have a similar metabolism but do not accumulate P. Most research to date has focused on the use of certain volatile fatty acids (VFAs) as carbon sources and on process conditions at temperatures common in temperate climates. Much remains unknown about the potential of EBPR in tropical regions and the suitability of other carbon substrates to drive the accumulation of phosphate. The purpose of this dissertation is to contribute to the understanding of EBPR at high temperatures and with unconventional carbon sources. Three different studies were designed and conducted with the following aims: (a) to evaluate the long-term EBPR stability and key microbial community in a wastewater treatment plant (WWTP) designed to achieve P removal in Singapore, (b) to study the process efficiency, biochemical transformations and organisms involved in a laboratory-scale EBPR reactor fed by alternating the substrates acetate and glutamate, and (c) to assess the potential of using unconventional carbon sources for EBPR by testing glutamate and glucose as alternating substrates at the laboratory-scale. The research included experiments at the full- and laboratory-scale, all at a mean temperature of 30 °C. Sustained observations in all three studies served to uncover the biochemical and microbial community dynamics. In the full-scale study, I conducted a yearlong evaluation of the EBPR activity at a WWTP that had been retrofitted to facilitate EBPR in Singapore. A mean P removal efficiency of 90 % was observed throughout the sampling period, similar to temperate climate installations and contrary to earlier reports that EBPR was not feasible at high temperatures. The main PAOs present in the reactor were Tetrasphaera, Candidatus Accumulibacter (Accumulibacter) and Dechloromonas, with mean relative abundances of 1.53, 0.43 and 0.69 %, respectively. The PAO community underwent changes during the surveyed period, with a marked transition from a Tetrasphaera-dominated community to a more even one. The link between PAOs and the P released in the anaerobic compartment was supported by a statistically significant correlation between the relative abundance of these organisms and the measured P concentrations. GAOs and PAOs coexisted without compromising the EBPR activity. In one of the laboratory-scale studies, glutamate and acetate were alternated as the carbon source for a reactor operated at 30 °C. Complete and stable P removal was achieved with a predominantly glutamate-containing feed, after modifying operating parameters commonly used in VFA-fed systems to a COD/P ratio of 40:1 mg COD/mg P and a cycle duration of 8 h. Long-term EBPR with a feed dominated by glutamate in a laboratory-scale reactor has not been previously reported. The P and carbon cycling patterns were different for glutamate and acetate. Reduced P release and polyhydroxyalkanoate (PHA) accumulation happened when glutamate was fed, but not with acetate, where glutamate appeared to be stored as an unidentified non-PHA compound or as different compounds. The PAO Accumulibacter and the GAO Candidatus Competibacter (Competibacter) remained the only known EBPR bacteria during the period of good EBPR performance, at similar relative abundances. A canonical correlation analysis revealed that the relative abundance of some non-PAO organisms correlated more strongly with variables that denoted good EBPR activity than did the abundance of any of the known PAOs. In the last study, a laboratory-scale sequencing batch reactor was used to test the EBPR potential of glutamate and glucose as alternating carbon sources in a high temperature process. The recommended influent COD/P ratio and batch duration for VFA-fed systems were unsuccessful. After modifications, COD/P ratios of 20:1 and 40:1 mg COD/ mg P resulted in complete P removal, but only in the short term. The EBPR stoichiometry with these two carbon substrates differed from that of VFA-fed systems. For both, lower P and PHA cycling was observed, and intracellular carbon storage compounds that were not PHA appeared to contribute to P cycling, as shown from carbon balances. A very diverse EBPR community was present in the reactor, including Accumulibacter, Tetrasphaera and Dechloromonas PAOs, and Competibacter, Defluviicoccus, Micropruina and Kineosphaera GAOs. Most of these organisms have not been reported before in laboratory-scale EBPR reactors operated at high temperatures. The work presented in this dissertation expands the understanding of EBPR by showing that the process is possible and stable in full-scale treatment plants at high temperature, with removal efficiencies similar to those observed in temperate climates. In addition, it was shown that unconventional carbon sources, specifically, glutamate and glucose, do participate in EBPR and that complete and stable phosphorus removal can be achieved with glutamate as dominant substrate at high temperature. A core PAO and GAO community was present in the three systems, where the interactions among members were more complex than previously considered, including competition, coexistence and succession events. The results obtained from this work enhance our fundamental knowledge of EBPR as an industrial process, as well as the metabolic diversity, niches and dynamics of PAOs and GAOs. The study outcomes can inform design and operational strategies at full-scale treatment plants. Lastly, the consideration of both high temperatures and unconventional carbon sources for EBPR is expected to aid in the development of more efficient treatment processes.
Current Developments In Biotechnology And Bioengineering
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Author : Sunil Kumar
language : en
Publisher: Elsevier
Release Date : 2021-06-16
Current Developments In Biotechnology And Bioengineering written by Sunil Kumar and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-06-16 with Technology & Engineering categories.
Strategic Perspectives in Solid Waste and Wastewater Management explores conventional and advanced biotechnologies for waste management, including socio-economic aspects, techno-economic feasibility, models and modeling tools, and a detailed life-cycle assessment approach in solid waste (SW) and wastewater (WW). These innovative technologies are highly applicable to current real-world situations. The enormous increase in the quantum and diversity of SW and WW - including waste materials generated due to human activity and their potentially harmful effects on the environment and public health - have led to increasing awareness about an urgent need to adopt novel technologies for appropriate management of both SW and WW. While there is an obvious need to minimize the generation of wastes and to reuse and recycle them, the technologies for managing such wastes can play a vital role in mitigating problems. Besides recovery of substantial energy, these technologies can lead to a considerable reduction in the overall waste quantities requiring final disposal, which can be better managed for safe disposal in a controlled manner while meeting pollution control standards. Outlines appropriate technologies for solid waste and wastewater management systems and their applications Presents and evaluates the Best Available Technology (BAT) and includes global case studies Provides methods for evaluating the way to use appropriate technological systems to develop the best technically and economically feasible projects worldwide Offers an excellent resource for university students to use for their research and dissertations
Aerobic Granular Sludge
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Author : S. Bathe
language : en
Publisher: IWA Publishing
Release Date : 2005-03-31
Aerobic Granular Sludge written by S. Bathe and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2005-03-31 with Science categories.
Aerobic Granular Sludge has recently received growing attention by researchers and technology developers, worldwide. Laboratory studies and preliminary field tests led to the conclusion that granular activated sludge can be readily established and profitably used in activated sludge plants, provided 'correct' process conditions are chosen. But what makes process conditions 'correct'? And what makes granules different from activated sludge flocs? Answers to these question are offered in Aerobic Granular Sludge. Major topics covered in this book include: Reasons and mechanism of aerobic granule formation Structure of the microbial population of aerobic granules Role, composition and physical properties of EPS Diffuse limitation and microbial activity within granules Physio-chemical characteristics Operation and application of granule reactors Scale-up aspects of granular sludge reactors, and case studies Aerobic Granular Sludge provides up-to-date information about a rapidly emerging new technology of biological treatment.
Phosphorus Polluter And Resource Of The Future
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Author : Christian Schaum
language : en
Publisher: IWA Publishing
Release Date : 2018-03-15
Phosphorus Polluter And Resource Of The Future written by Christian Schaum and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-03-15 with Science categories.
This comprehensive book provides an up-to-date and international approach that addresses the Motivations, Technologies and Assessment of the Elimination and Recovery of Phosphorus from Wastewater. This book is part of the Integrated Environmental Technology Series.
Process Design Manual For Nitrogen Control
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Author : United States. Environmental Protection Agency. Office of Technology Transfer
language : en
Publisher:
Release Date : 1975
Process Design Manual For Nitrogen Control written by United States. Environmental Protection Agency. Office of Technology Transfer and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1975 with Nitrification categories.
Innovative Wastewater Treatment Resource Recovery Technologies Impacts On Energy Economy And Environment
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Author : Juan M. Lema
language : en
Publisher: IWA Publishing
Release Date : 2017-06-15
Innovative Wastewater Treatment Resource Recovery Technologies Impacts On Energy Economy And Environment written by Juan M. Lema and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-06-15 with Science categories.
This book introduces the 3R concept applied to wastewater treatment and resource recovery under a double perspective. Firstly, it deals with innovative technologies leading to: Reducing energy requirements, space and impacts; Reusing water and sludge of sufficient quality; and Recovering resources such as energy, nutrients, metals and chemicals, including biopolymers. Besides targeting effective C,N&P removal, other issues such as organic micropollutants, gases and odours emissions are considered. Most of the technologies analysed have been tested at pilot- or at full-scale. Tools and methods for their Economic, Environmental, Legal and Social impact assessment are described. The 3R concept is also applied to Innovative Processes design, considering different levels of innovation: Retrofitting, where novel units are included in more conventional processes; Re-Thinking, which implies a substantial flowsheet modification; and Re-Imagining, with completely new conceptions. Tools are presented for Modelling, Optimising and Selecting the most suitable plant layout for each particular scenario from a holistic technical, economic and environmental point of view.
Activated Sludge Models
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Author : The IWA Task Group on Mathematical Modelling for Design and Operation of Biological Wastewater Treatment
language : en
Publisher: IWA Publishing
Release Date : 2000-05-31
Activated Sludge Models written by The IWA Task Group on Mathematical Modelling for Design and Operation of Biological Wastewater Treatment and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2000-05-31 with Science categories.
This book has been produced to give a total overview of the Activated Sludge Model (ASM) family at the start of 2000 and to give the reader easy access to the different models in their original versions. It thus presents ASM1, ASM2, ASM2d and ASM3 together for the first time. Modelling of activated sludge processes has become a common part of the design and operation of wastewater treatment plants. Today models are being used in design, control, teaching and research. Contents ASM3: Introduction, Comparison of ASM1 and ASM3, ASM3: Definition of compounds in the model, ASM3: Definition of processes in the Model, ASM3: Stoichiometry, ASM3: Kinetics, Limitations of ASM3, Aspects of application of ASM3, ASM3C: A Carbon based model, Conclusion ASM 2d: Introduction, Conceptual Approach, ASM 2d, Typical Wastewater Characteristics and Kinetic and Stoichiometric Constants, Limitations, Conclusion ASM 2: Introduction, ASM 2, Typical Wastewater Characteristics and Kinetic and Stoichiometric Constants, Wastewater Characterization for Activated Sludge Processes, Calibration of the ASM 2, Model Limitations, Conclusion, Bibliography ASM 1: Introduction, Method of Model Presentation, Model Incorporating Carbon Oxidation Nitrification and Denitrification, Characterization of Wastewater and Estimation of Parameter Values, Typical Parameter Ranges, Default Values, and Effects of Environmental Factors, Assumptions, Restrictions and Constraints, Implementation of the Activated Sludge Model Scientific and Technical Report No.9
Waste Water Treatment Technologies Volume Ii
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Author : Saravanamuthu Vigneswaran
language : en
Publisher: EOLSS Publications
Release Date : 2009-09-25
Waste Water Treatment Technologies Volume Ii written by Saravanamuthu Vigneswaran and has been published by EOLSS Publications this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-09-25 with categories.
Water and Wastewater Treatment Technologies theme is a component of Encyclopedia of Water Sciences, Engineering and Technology Resources in the global Encyclopedia of Life Support Systems (EOLSS), which is an integrated compendium of twenty one Encyclopedias. The Theme on Water and Wastewater Treatment Technologies deals, in three volumes, and covers several topics, with several issues of great relevance to our world such as: Urban Wastewater Treatment; Characteristics of Effluent Organic Matter in Wastewater; Filtration Technologies in wastewater treatment; Air Stripping in Industrial Wastewater Treatment; Dissolved air flotation in industrial wastewater treatment; Membrane Technology for Organic Removal in Wastewater; Adsorption and Biological Filtration in Wastewater Treatment; Physico-chemical processes for Organic removal from wastewater effluent; Deep Bed Filtration: Modelling Theory And Practice ; Specific options in biological wastewater treatment for reclamation and reuse ; Biological Phosphorus Removal Processes For Wastewater Treatment ; Sequencing Batch Reactors: Principles, Design/Operation And Case Studies ; Wastewater stabilization ponds (WSP)for wastewater treatment; Treatment of industrial wastewater by membrane bioreactors; Stormwater treatment technologies; Sludge Treatment Technologies ; Wastewater Treatment Technology For Tanning Industry; Palm Oil And Palm Waste Potential In Indonesia ; Recirculating Aquaculture Systems – A Review ; Upflow anaerobic sludge blanket (UASB)reactor in wastewater treatment; Applied Technologies In Municipal Solid Waste Landfill Leachate Treatment; Water Mining: Planning and Implementation Issues for a successful project; Assessment methodologies for water reuse scheme and technology; Nanotechnology for Wastewater Treatment. These three volumes are aimed at the following five major target audiences: University and College students Educators, Professional practitioners, Research personnel and Policy analysts, Managers, and Decision makers and NGOs.
Inorganic Polyphosphates
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Author : Heinz C. Schröder
language : en
Publisher: Springer
Release Date : 2012-10-12
Inorganic Polyphosphates written by Heinz C. Schröder and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-10-12 with Science categories.
Inorganic polyphosphates - polymers of orthophosphate linked by high-energy phosphoanhydride bonds - have been found in apparently all forms of life, from bacteria, yeasts and fungi to higher plants and animals. These polymers, which had been neglected for a long time, have become a fascinating area of research in the last few years. This volume summarizes the present state of knowledge about the metabolism and function of inorganic polyphosphates. In addition, the methods to study these polymers as well as the biotechnological applications of inorganic polyphosphates are described. The 15 chapters of this volume, dealing with different aspects of polyphosphate research, are written by experts in the field. This book represents a valuable source of information not only for researchers working on this subject, but also for scientists interested in fundamental aspects of cell and energy metabolism.
Handbook Of Biological Wastewater Treatment
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Author : Adrianus van Haandel
language : en
Publisher: IWA Publishing
Release Date : 2012-02-20
Handbook Of Biological Wastewater Treatment written by Adrianus van Haandel and has been published by IWA Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-02-20 with Science categories.
The scope of this comprehensive new edition of Handbook of Biological Wastewater Treatment ranges from the design of the activated sludge system, final settlers, auxiliary units (sludge thickeners and digesters) to pre-treatment units such as primary settlers and UASB reactors. The core of the book deals with the optimized design of biological and chemical nutrient removal. The book presents the state-of-the-art theory concerning the various aspects of the activated sludge system and develops procedures for optimized cost-based design and operation. It offers a truly integrated cost-based design method that can be easily implemented in spreadsheets and adapted to the particular needs of the user. Handbook of Biological Wastewater Treatment: Second Edition incorporates valuable new material that improves the instructive qualities of the first edition. The book has a new structure that makes the material more readily understandable and the numerous additional examples clarify the text. On the website www.wastewaterhandbook.com three free excel design spreadsheets for different configurations (secondary treatment with and without primary settling and nitrogen removal) can be downloaded to get the reader started with their own design projects. New sections have been added throughout: to explain the difference between true and apparent yield while the section on the F/M ratio, and especially the reasons not to use it, has been expanded; to demonstrate the effect of the oxygen recycle to the anoxic zones on both the denitrification capacity and the concept of available nitrate is explained in more detail. the latest developments on the causes and solution to sludge bulking and scum formation to show the rapid developments of innovative nitrogen removal and sludge separation problems the anaerobic pre-treatment section is completely rewritten based on the experiences obtained from an extensive review of large full-scale UASB based sewage treatment plants a new section on industrial anaerobic wastewater treatment three new appendices have been added. These deal with the calibration of the denitrification model, empirical design guidelines for final settler design (STORA/STOWA and ATV) and with the potential for development of denitrification in the final settler. A new chapter on moving bed biofilm reactors Handbook of Biological Wastewater Treatment: Second Edition is written for post graduate students and engineers in consulting firms and environmental protection agencies. It is an invaluable resource for everybody working in the field of wastewater treatment. Lecturer support material is available when adopted for university courses. This includes course material for the first 7 modules in the form of PDF printouts and an exercise file with questions and answers and a symbol list. Authors: Prof. dr. ir. A.C. van Haandel, Federal University of Campina Grande - Brazil and Ir. J.G.M. van der Lubbe, Biothane Systems International - Veolia, The Netherlands