Experimental Manipulation Of Gene Expression
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Experimental Manipulation Of Gene Expression
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Author : Masayori Inouye
language : en
Publisher: Academic Press
Release Date : 2014-06-28
Experimental Manipulation Of Gene Expression written by Masayori Inouye and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-06-28 with Science categories.
Experimental Manipulation of Gene Expression discusses a wide range of host systems in which to clone and express a gene of interest. The aims are for readers to quickly learn the versatility of the systems and obtain an overview of the technology involved in the manipulation of gene expression. Furthermore, it is hoped that the reader will learn enough from the various approaches to be able to develop systems and to arrange for a gene of particular interest to express in a particular system. The book opens with a chapter on the design and construction of a plasmid vector system used to achieve high-level expression of a particular phage regulatory protein normally found in minute amounts in a phage-infected bacterial cell. This is followed by separate chapters on topics such as high-level expression vectors that utilize efficient Escherichia coli lipoprotein promoter as well as various other portions of the lipoprotein gene Ipp; DNA cloning systems for streptomycetes; and the design and application of vectors for high-level, inducible synthesis of the product of a cloned gene in yeast.
Experimental Manipulation Of Gene Expression
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Author : M Inouye (Ed)
language : en
Publisher:
Release Date : 1983
Experimental Manipulation Of Gene Expression written by M Inouye (Ed) and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1983 with categories.
Epigenetic Gene Expression And Regulation
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Author : Suming Huang
language : en
Publisher: Academic Press
Release Date : 2015-10-19
Epigenetic Gene Expression And Regulation written by Suming Huang and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2015-10-19 with Science categories.
Epigenetic Gene Expression and Regulation reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies. The book shows how these heritable mechanisms allow individual cells to establish stable and unique patterns of gene expression that can be passed through cell divisions without DNA mutations, thereby establishing how different heritable patterns of gene regulation control cell differentiation and organogenesis, resulting in a distinct human organism with a variety of differing cellular functions and tissues. The work begins with basic biology, encompasses methods, cellular and tissue organization, topical issues in epigenetic evolution and environmental epigenesis, and lastly clinical disease discovery and treatment. Each highly illustrated chapter is organized to briefly summarize current research, provide appropriate pedagogical guidance, pertinent methods, relevant model organisms, and clinical examples. - Reviews current knowledge on the heritable molecular mechanisms that regulate gene expression, contribute to disease susceptibility, and point to potential treatment in future therapies - Helps readers understand how epigenetic marks are targeted, and to what extent transgenerational epigenetic changes are instilled and possibly passed onto offspring - Chapters are replete with clinical examples to empower the basic biology with translational significance - Offers more than 100 illustrations to distill key concepts and decipher complex science
Cloning
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Author : Stephen D. Fairbanks
language : en
Publisher: Nova Publishers
Release Date : 2004
Cloning written by Stephen D. Fairbanks and has been published by Nova Publishers this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Science categories.
The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Seed Development And Germination
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Author : Jaime Kigel
language : en
Publisher: Routledge
Release Date : 2017-11-01
Seed Development And Germination written by Jaime Kigel and has been published by Routledge this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-11-01 with Science categories.
This text is intended for plant physiologists, molecular biologists, biochemists, biotechnologists, geneticists, horticulturalists, agromnomists and botanists, and upper-level undergraduate and graduate students in these disciplines. It integrates advances in the diverse and rapidly-expanding field of seed science, from ecological and demographic aspects of seed production, dispersal and germination, to the molecular biology of seed development. The book offers a broad, multidisciplinary approach that covers both theoretical and applied knowledge.
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2004
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.
Handbook Of Research On Computational Methodologies In Gene Regulatory Networks
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Author : Das, Sanjoy
language : en
Publisher: IGI Global
Release Date : 2009-10-31
Handbook Of Research On Computational Methodologies In Gene Regulatory Networks written by Das, Sanjoy and has been published by IGI Global this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-10-31 with Computers categories.
"This book focuses on methods widely used in modeling gene networks including structure discovery, learning, and optimization"--Provided by publisher.
Gene Cloning And Manipulation
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Author : Christopher Howe
language : en
Publisher: Cambridge University Press
Release Date : 2007-07-12
Gene Cloning And Manipulation written by Christopher Howe and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-07-12 with Medical categories.
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
Rna Methodologies
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Author : Robert E. Farrell Jr.
language : en
Publisher: Elsevier
Release Date : 2022-11-22
Rna Methodologies written by Robert E. Farrell Jr. and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-11-22 with Science categories.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. - Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology - Builds from basic information on RNA techniques to in-depth protocols for specific applications - Features new chapters on RNA sequencing and RNA in situ hybridization - Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus - Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics
Bios Instant Notes In Developmental Biology
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Author : Dr Richard Twyman
language : en
Publisher: Taylor & Francis
Release Date : 2023-05-31
Bios Instant Notes In Developmental Biology written by Dr Richard Twyman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2023-05-31 with Science categories.
Coverage of the field in Instant Notes in Developmental Biology is current and focuses largely on the principles of embryonic development. It is designed to provide a clear summary of the principles of developmental biology in a compact and easily manageable structure.