Extracellular Matrix Protocols
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Extracellular Matrix Protocols
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Author : Charles Streuli
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-05
Extracellular Matrix Protocols written by Charles Streuli and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-05 with Science categories.
It is now widely accepted that much of the dynamic function of cells and tissues is regulated from outside the cell by the extracellular matrix. In ad- tion to its conventional role in providing a scaffold for building tissues, the extracellular matrix acts as a directional highway for cellular movement and provides instructional information for promoting survival, proliferation, and differentiation. Indeed, the extracellular matrix is beginning to take a starring role in the choreography of cell and tissue function. The diverse roles of the extracellular matrix are reflected in its highly complicated structure, consisting of an ever increasing number of components. Yet the mechanisms of extracellular matrix assembly and how they influences cell behavior are only just beginning to be understood. In order to solve these problems new methodologies are, of necessity, being developed. Many of these technologies are highly sophisticated and are currently available only in a ha- ful of laboratories. However, we believe that they can readily be transported and established by other researchers. Thus, the purpose of Extracellular Matrix Protocols is to present some of these complicated techniques in a style that is relatively easy to reproduce.
Extracellular Matrix Protocols
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Author : Sharona Even-Ram
language : en
Publisher: Humana Press
Release Date : 2009-07-24
Extracellular Matrix Protocols written by Sharona Even-Ram and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2009-07-24 with Medical categories.
The study of the extracellular matrix (ECM) and its diverse roles in tissue scaffolding and cellular signaling in both physiological and pathological processes has significantly expanded over the past decade. Although well appreciated, the structural and biochemical complexity and the dynamic nature of the living matrix are still under extensive inv- tigation, yielding a growing number of methods with varying degree of sophistication and intricacy. In this edition of Extracellular Matrix Protocols, we compiled a variety of methods that can be readily reproduced in most cell biology laboratories, as well as several cutting-edge technologies that are indeed available in a limited number of centers, but are well worth the awareness and exposure to the ECM research community. As in the previous edition, the book chapters are divided into sections that rep- sent molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, cell biology methods for the asse- ment of cell behavior and matrix assembly and tissue engineering applications. All chapters were contributed by scientists who developed the methods or mastered and perfected methods that were routinely used in their laboratories. An effort was made to provide practical working details and helpful notes for the nonexpert user in order to assist reproducibility and accuracy. We hope that these valuable protocols will become helpful tools for ECM researchers and will be further developed and tailored to the s- cific needs of a growing number of applications.
The Extracellular Matrix
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Author : Davide Vigetti
language : en
Publisher: Humana Press
Release Date : 2019-03-31
The Extracellular Matrix written by Davide Vigetti and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-03-31 with Science categories.
This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.
Extracellular Matrix
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Author : Jennie B. Leach
language : en
Publisher: Humana
Release Date : 2016-08-23
Extracellular Matrix written by Jennie B. Leach and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-08-23 with Medical categories.
This volume provides comprehensive procedures for analyzing the extracellular matrix in native, injured, and engineered neural tissues. Divided into four parts, each focusing on different aspects of the extracellular matrix and the nervous system, Extracellular Matrix covers methods to analyze native tissue, in vitro models for investigating cell-extracellular matrix interactions in a variety of contexts, protocols to investigate the role of the extracellular matrix in nervous system injury, degeneration, and regeneration, as well as therapeutics and engineered systems. Each chapter is written by leading experts and presents established protocols in a concise format, encompassing current technologies as well as methods developed over years of research. Beginning with an introduction to the method, chapters continue with a listing of the materials and equipment, step-by-step protocols, and a Notes section with troubleshooting tips, supplemental details and alternative approaches, as well as a list of references for further information. As part of the practical and convenient Neuromethods series, Extracellular Matrix serves as an invaluable aid for researchers studying this vital area of neuroscience.
Methods In Cell Matrix Adhesion
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Author : Josephine Adams
language : en
Publisher: Academic Press
Release Date : 2002-06-12
Methods In Cell Matrix Adhesion written by Josephine Adams and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2002-06-12 with Science categories.
Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.
Integrin Protocols
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Author : Anthony R. Howlett
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Integrin Protocols written by Anthony R. Howlett and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. The emphasis is always on the practical steps necessary for experimental success and robust results. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis, as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.
The Extracellular Matrix An Overview
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Author : Robert Mecham
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-02-16
The Extracellular Matrix An Overview written by Robert Mecham and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-02-16 with Science categories.
Knowledge of the extracellular matrix (ECM) is essential to understand cellular differentiation, tissue development, and tissue remodeling. This volume of the series “Biology of Extracellular Matrix” provides a timely overview of the structure, regulation, and function of the major macromolecules that make up the extracellular matrix. It covers topics such as collagen types and assembly of collagen-containing suprastructures, basement membrane, fibronectin and other cell-adhesive glycoproteins, proteoglycans, microfibrils, elastin, fibulins and matricellular proteins, such as thrombospondin. It also explores the concept that ECM components together with their cell surface receptors can be viewed as intricate nano-devices that allow cells to physically organize their 3-D-environment. Further, the role of the ECM in human disease and pathogenesis is discussed as well as the use of model organisms in elucidating ECM function.
Cell Biology Of Extracellular Matrix
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Author : E.D. Hay
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-11-11
Cell Biology Of Extracellular Matrix written by E.D. Hay and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-11-11 with Science categories.
In the ten-year interval since the first edition of this volume went to press, our knowledge of extracellular matrix (ECM) function and structure has enor mously increased. Extracellular matrix and cell-matrix interaction are now routine topics in the meetings and annual reviews sponsored by cell biology societies. Research in molecular biology has so advanced the number of known matrix molecules and the topic of gene structure and regulation that we won dered how best to incorporate the new material. For example, we deliberated over the inclusion of chapters on molecular genetics. We decided that with judicious editing we could present the recent findings in molecular biology within the same cell biology framework that was used for the first edition, using three broad headings: what is extracellular matrix, how is it made, and what does it do for cells? Maintaining control over the review of literature on the subject of ECM was not always an easy task, but we felt it was essential to production of a highly readable volume, one compact enough to serve the the student as an introduction and the investigator as a quick update on graduate the important recent discoveries. The first edition of this volume enjoyed con hope the reader finds this edition equally useful. siderable success; we D. Hay Elizabeth vii Contents Introductory Remarks 1 Elizabeth D. Hay PART I. WHAT IS EXTRACELLULAR MATRIX? Chapter 1 Collagen T. F. Linsenmayer 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 2. The Collagen Molecule . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 2. 1. Triple-Helical Domain(s) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Holistic Medicine And The Extracellular Matrix
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Author : Matthew Wood
language : en
Publisher: Simon and Schuster
Release Date : 2021-09-28
Holistic Medicine And The Extracellular Matrix written by Matthew Wood and has been published by Simon and Schuster this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-09-28 with Health & Fitness categories.
• Examines the function of the extracellular matrix, the inner ocean that unifies all our cells and controls them in a coordinated and integrated fashion • Explores how the extracellular matrix builds and repairs itself and how holistic therapy can be applied based on this knowledge • Introduces new and old holistic and herbal protocols for treatment of the matrix The cells in our bodies are not independent units. They do not control their own feeding, elimination, migration, or reproduction; they are controlled by signals from the extracellular matrix (ECM) that surrounds them. This all-encompassing inner ocean unifies all our cells and controls them in a coordinated and integrated fashion. Revealing the stunning implications of the extracellular matrix, Matthew Wood shows how it clearly explains the actions and efficacy of holistic therapies. He explores the groundbreaking research of Alfred Pischinger, who discovered the ECM in 1975, as well as the role of the matrix in transmitting and enacting the genetic code, including the roles of the mitochondria, the nucleus, and ribosomes. Wood explains how modern drugs, directed at specific receptors on the cell membrane, interfere with bodily self-regulation. He details how holistic therapies modify the environment of the cell and strengthen the whole, bringing the body back to homeostasis and consequently offering true healing.
Tissue Engineering
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Author : Narine Sarvazyan
language : en
Publisher: Springer Nature
Release Date : 2020-04-02
Tissue Engineering written by Narine Sarvazyan and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-04-02 with Medical categories.
Tissue engineering and regenerative medicine is a new, interdisciplinary branch of science that combines knowledge from numerous scientific fields including biology, biochemistry, physics, chemistry, applied engineering, and medicine. It aims to restore damaged parts of the human body by rebuilding them in vitro using individual building blocks of biological tissues such as cells and the extracellular matrix that surrounds them. The authors hope to spark students’ interest in this exciting new field of science as well as give them a basic knowledge of its terminology. This book is based on a hands-on practical course in tissue engineering conducted by the Fulbright US Scholar recipient, Dr. Narine Sarvazyan (George Washington University, Washington USA). It provides an overview of the core topics of the tissue engineering field, including stem cell differentiation, the role of extracellular matrix and attachment proteins, scaffolds, and culturing of engineered tissues. Each chapter is accompanied by hands-on demonstrations and self-check questions. The text is easily readable for students of all backgrounds and the described protocols can be conducted using common lab equipment. This textbook is also useful for developing undergraduate and graduate courses that teach basic methods and approaches in this promising and rapidly developing field.