Fed Batch Cultures
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Fed Batch Cultures
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Author : Henry C. Lim
language : en
Publisher: Cambridge University Press
Release Date : 2013-04-22
Fed Batch Cultures written by Henry C. Lim and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-22 with Technology & Engineering categories.
Many, if not most, industrially important fermentation and bioreactor operations are carried out in fed-batch mode, producing a wide variety of products. In spite of this, there is no single book that deals with fed-batch operations. This is the first book that presents all the necessary background material regarding the 'what, why and how' of optimal and sub-optimal fed-batch operations. Numerous examples are provided to illustrate the application of optimal fed-batch cultures. This unique book, by world experts with decades of research and industrial experience, is a must for researchers and industrial practitioners of fed-batch processes (modeling, control and optimization) in biotechnology, fermentation, food, pharmaceuticals and waste treatment industries.
Cell Culture Engineering
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Author : Gyun Min Lee
language : en
Publisher: John Wiley & Sons
Release Date : 2020-01-13
Cell Culture Engineering written by Gyun Min Lee and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-13 with Science categories.
Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
Fed Batch Cultures
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Author : Henry C. Lim. Hwa Sung Shin
language : en
Publisher:
Release Date : 2013
Fed Batch Cultures written by Henry C. Lim. Hwa Sung Shin and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013 with categories.
Cell Culture Engineering
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Author : Wei-Shu Hu
language : en
Publisher: Springer
Release Date : 2006-08-16
Cell Culture Engineering written by Wei-Shu Hu and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-08-16 with Science categories.
Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.
Feeding Strategies For Fed Batch Cultures Of Animal Cells
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Author : Jan-Oliver Schwabe
language : en
Publisher:
Release Date : 2001
Feeding Strategies For Fed Batch Cultures Of Animal Cells written by Jan-Oliver Schwabe and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2001 with categories.
Recombinant Glycoprotein Production
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Author : Virgínia Picanço-Castro
language : en
Publisher: Humana
Release Date : 2018-08-18
Recombinant Glycoprotein Production written by Virgínia Picanço-Castro and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2018-08-18 with Science categories.
This volume covers a wide spectrum of techniques and approaches that are used in the upstream and downstream processing for recombinant glycoprotein production. Chapters guide the reader through state-of-art of therapeutic recombinant glycoproteins, explores the patent literature, expression systems used for glycoproteins production, methods employed in the downstream processing of different glycoproteins, and information about analytical tools and formulation strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Glycoprotein Production: Methods and Protocols aims to ensure successful results in the further study of this vital field
Physiological Engineering Aspects Of Penicillium Chrysogenum
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Author : Jens Nielsen
language : en
Publisher: World Scientific
Release Date : 1997
Physiological Engineering Aspects Of Penicillium Chrysogenum written by Jens Nielsen and has been published by World Scientific this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997 with Medical categories.
The book gives a review of penicillin production by Penicillium chrysogenum, and also deals with a number of general aspects of fungal cultivations, e.g. primary metabolism of filamentous fungi, morphology, monitoring of fungal cultivations, and bioreactor performance (more than 750 references).The first two chapters give an introduction to the area of penicillin production; with a review of the history and a survey of the present status of this industrially very important process in the first chapter. In the second chapter is given an introduction to the microorganism, i.e. its nutritional requirements, its taxonomy, and an overview of different strain development programmes.Chapter 3 gives an introduction to the concept of Physiological Engineering. This is followed by a review of various monitoring techniques and different theoretical techniques for analysis of cultivation processes, e.g. mathematic modeling, metabolic flux analysis, and metabolic control analysis.Chapter 4 and 5 give a review of the metabolism, with the primary metabolism being the topic of Chapter 4 and the secondary metabolism, i.e. penicillin biosynthesis, being the topic of Chapter 5. The review of the penicillin biosynthetic pathway is followed by a description of a number of results obtained using metabolic flux and metabolic control analysis.Chapter 6 is devoted to the morphology of the fungus, and it gives a detailed description of the growth mechanisms of filamentous fungi.Chapter 7 deals with the bioreactor performance during fungal cultivations, i.e. medium rheology, gas-liquid mass transfer, and mixing.Finally is the fed-batch process applied for penicillin production described in Chapter 8. It gives an overview of the most important factors influencing penicillin production.
Fed Batch Fermentation
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Author : G G Moulton
language : en
Publisher: Woodhead Publishing
Release Date : 2014-09-29
Fed Batch Fermentation written by G G Moulton and has been published by Woodhead Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-09-29 with Science categories.
Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.
Fed Batch Fermentation
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Author : G G Moulton
language : en
Publisher: Elsevier
Release Date : 2014-10-16
Fed Batch Fermentation written by G G Moulton and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-16 with Science categories.
Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies. This process can be used as a teaching tool for the inexperienced fermentation student or researcher in the fields of bioprocessing and bioreactors. It is an important segue from E. coli shake flask cultures to bioreactor The fed-batch fermentation is designed to be accomplished in a single day with the preparation work being done on the day prior The fed-batch fermentation described in this book is a robust process and can be easily scaled for CMO production of protein product
Modern Tools And Techniques To Understand Microbes
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Author : Ajit Varma
language : en
Publisher: Springer
Release Date : 2017-04-21
Modern Tools And Techniques To Understand Microbes written by Ajit Varma and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2017-04-21 with Science categories.
This book provides essential molecular techniques and protocols for analyzing microbes that are useful for developing novel bio-chemicals, such as medicines, biofuels, and plant protection substances. The topics and techniques covered include: microbial diversity and composition; microorganisms in the food industry; mass cultivation of sebacinales; host-microbe interaction; targeted gene disruption; function-based metagenomics to reveal the rhizosphere microbiome; mycotoxin biosynthetic pathways; legume-rhizobium symbioses; multidrug transporters of yeast; drug-resistant bacteria; the fungal endophyte piriformospora indica; medicinal plants; arbuscular mycorrhizal fungi; biosurfactants in microbial enhanced oil recovery; and biocontrol of the soybean cyst nematode with root endophytic fungi; as well as microbe-mediated drought tolerance in plants.