Flow Cytometry Applications In Cell Culture
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Flow Cytometry Applications In Cell Culture
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Author : Mohamed Al-Rubeai
language : en
Publisher: CRC Press
Release Date : 2020-07-24
Flow Cytometry Applications In Cell Culture written by Mohamed Al-Rubeai and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-07-24 with Science categories.
This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.
Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration
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Author : Awtar Krishan
language : en
Publisher: John Wiley and Sons
Release Date : 2011-05-12
Applications Of Flow Cytometry In Stem Cell Research And Tissue Regeneration written by Awtar Krishan and has been published by John Wiley and Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-05-12 with Science categories.
A much-needed primer on the use of laser flow cytometry for stem cell analysis Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells. Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction. Chapter coverage includes: Stem cell analysis and sorting using side population Flow cytometry in the study of proliferation and apoptosis Stem cell biology and application Identification and isolation of very small embryonic-like stem cells from murine and human specimens Hematopoietic stem cells—issues in enumeration Human embryonic stem cells: long-term culture and cardiovascular differentiation Limbal stem cells and corneal regeneration Flow cytometric sorting of spermatogonial stem cells Breast cancer stem cells Stem cell marker expression in cells from body cavity fluids This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.
Advanced Flow Cytometry Applications In Biological Research
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Author : R.C. Sobti
language : en
Publisher: Springer Science & Business Media
Release Date : 2013-04-17
Advanced Flow Cytometry Applications In Biological Research written by R.C. Sobti and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2013-04-17 with Medical categories.
Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.
Flow Cytometry
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Author : Marion G. Macey
language : en
Publisher: Springer Science & Business Media
Release Date : 2007-11-03
Flow Cytometry written by Marion G. Macey and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-11-03 with Medical categories.
Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers. The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. The book charts the history, development and basic principles of flow cytometry.
Flow Cytometry
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Author : Andrew Yen
language : en
Publisher: CRC Press
Release Date : 1989-08-31
Flow Cytometry written by Andrew Yen and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1989-08-31 with Science categories.
The current technology and its applications in flow cytometry are presented in this comprehensive reference work. Described in explicit detail are the instrumentation and its components, and applications of the technology in cell biology, immunology, pharmacology, genetics, hematology and clinical medicine. Methods for data analysis, including both hardware and software, and explicit experimental techniques for making specific measurements are presented. Material is divided by topic into two volumes: Volume I covers instrumentation, genetics, and cell structure; Volume II contains material on cell function studies by flow cytometry. This reference is essential for both the novice and the experienced investigator using flow cytometry in research, and for students of cell biology, biomedical engineering, and medical technology.
Flow Cytometry Protocols
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Author : Teresa S. Hawley
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Flow Cytometry Protocols written by Teresa S. Hawley and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).
Clinical Flow Cytometry
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Author : Ingrid Schmid
language : en
Publisher: BoD – Books on Demand
Release Date : 2012-05-16
Clinical Flow Cytometry written by Ingrid Schmid and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-05-16 with Medical categories.
"Clinical Flow Cytometry - Emerging Applications" contains a collection of reviews and original papers that illustrate the relevance of flow cytometry for the study of specific diseases and clinical evaluations. The chapters have been contributed by authors from a wide variety of countries showing the broad application and importance of this technology in medicine. Examples include chapters on autoimmune disease, cancer, and the evaluation of new drugs. The book is intended to give newcomers a helpful introduction, but also to provide experienced flow cytometrists with novel insights and a better understanding of clinical cytometry.
Recent Advances In Cytometry Part A
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Author :
language : en
Publisher: Academic Press
Release Date : 2011-06-13
Recent Advances In Cytometry Part A written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-06-13 with Science categories.
Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology
Flow Cytometry Part B
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Author :
language : en
Publisher: Elsevier
Release Date : 1994-12-23
Flow Cytometry Part B written by and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-12-23 with Science categories.
From the Reviews of the First Edition: "This is a good reference manual for multi-user facility faced with a wide variety of biological applications." - CYTOMETRY "Flow Cytometry includes an impressive array of methods applicable to chromosome analysis, plant biology, marine biology, fluorescence, insitu hybridization, and others. It succeeds in providing the reader with good insight into the power of the technology throughout biology." - KENNETH A. AULT, MAINE CYTOMETRY RESEARCH INSTITUTE, MAINE MEDICAL CENTER, IN CANCER CELLS Flow Cytometry, Second Edition provides a complete and comprehensive two-volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who use flow Cytometry to separate and analyze cells. * * Comprehensive methodological coverage in unique style * In depth treatment of procedures * Description of each procedure's: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the technique * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry * 10 methods cover assessment of apoptosis and other modes of cell death * Practical, handbook-style presentation works in lab or classroom * Printed on acid-free paper * Color plates
Cell Separation Methods And Applications
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Author : Diether Recktenwald
language : en
Publisher: CRC Press
Release Date : 1997-11-04
Cell Separation Methods And Applications written by Diether Recktenwald and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1997-11-04 with Medical categories.
"Offers complete coverage and assessment of cell separation technologies for analytical and preparative isolations of biological cell populations-demonstrating how to select and devise optimal sorting strategies for applications in biochemistry, immunology, cell and molecular biology, and clinical research. "