Fluorescence Fluctuation Spectroscopy Techniques To Quantify Molecular Interactions And Dynamics In Complex Biological Systems
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Fluorescence Fluctuation Spectroscopy Techniques To Quantify Molecular Interactions And Dynamics In Complex Biological Systems
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Author : Valentin Dunsing
language : en
Publisher:
Release Date : 2020*
Fluorescence Fluctuation Spectroscopy Techniques To Quantify Molecular Interactions And Dynamics In Complex Biological Systems written by Valentin Dunsing and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020* with categories.
Living cells rely on transport and interaction of biomolecules to perform their diverse functions. A powerful toolbox to study these highly dynamic processes in the native environment is provided by fluorescence fluctuation spectroscopy (FFS) techniques. In more detail, FFS takes advantage of the inherent dynamics present in biological systems, such as diffusion, to infer molecular parameters from fluctuations of the signal emitted by an ensemble of fluorescently tagged molecules. In particular, two parameters are accessible: the concentration of molecules and their transit times through the observation volume. In addition, molecular interactions can be measured by analyzing the average signal emitted per molecule - the molecular brightness - and the cross-correlation of signals detected from differently tagged species. In the present work, several FFS techniques were implemented and applied in different biological contexts. In particular, scanning fluorescence correlation spectroscopy (sFCS) was performed to measure protein dynamics ...
Fluorescence Fluctuation Spectroscopy Ffs
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-12-29
Fluorescence Fluctuation Spectroscopy Ffs written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-29 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells
Fluorescence Fluctuation Spectroscopy Ffs
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-12-28
Fluorescence Fluctuation Spectroscopy Ffs written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-28 with Science categories.
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers fluorescence fluctuation spectroscopy Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
The Role Of Biomembranes And Biophysics In Immune Cell Signaling
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Author : Yan Shi
language : en
Publisher: Frontiers Media SA
Release Date : 2021-11-15
The Role Of Biomembranes And Biophysics In Immune Cell Signaling written by Yan Shi and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-11-15 with Medical categories.
Fluorescence Methods For Investigation Of Living Cells And Microorganisms
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Author : Natalia Grigoryeva
language : en
Publisher: BoD – Books on Demand
Release Date : 2020-09
Fluorescence Methods For Investigation Of Living Cells And Microorganisms written by Natalia Grigoryeva and has been published by BoD – Books on Demand this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-09 with Cytofluorometry categories.
Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Spectroscopy And The Dynamics Of Molecular Biological Systems
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Author : Peter M. Bayley
language : en
Publisher:
Release Date : 1985
Spectroscopy And The Dynamics Of Molecular Biological Systems written by Peter M. Bayley and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985 with Biopolymers categories.
Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications
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Author : Vicente Micó
language : en
Publisher: Frontiers Media SA
Release Date : 2021-10-22
Optical Microscopic And Spectroscopic Techniques Targeting Biological Applications written by Vicente Micó and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2021-10-22 with Science categories.
Single Molecule Dynamics In Life Science
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Author : Toshio Yanagida
language : en
Publisher: John Wiley & Sons
Release Date : 2008-11-24
Single Molecule Dynamics In Life Science written by Toshio Yanagida and has been published by John Wiley & Sons this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-11-24 with Science categories.
In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.
Advanced Fluorescence Reporters In Chemistry And Biology Iii
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Author : Alexander P. Demchenko
language : en
Publisher: Springer Science & Business Media
Release Date : 2011-03-29
Advanced Fluorescence Reporters In Chemistry And Biology Iii written by Alexander P. Demchenko and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2011-03-29 with Science categories.
The key element of any fluorescence sensing or imaging technology is the fluorescence reporter, which transforms the information on molecular interactions and dynamics into measurable signals of fluorescence emission. This book, written by a team of frontline researchers, demonstrates the broad field of applications of fluorescence reporters, starting from nanoscopic properties of materials, such as self-assembled thin films, polymers and ionic liquids, through biological macromolecules and further to living cell, tissue and body imaging. Basic information on obtaining and interpreting experimental data is presented and recent progress in these practically important areas is highlighted. The book is addressed to a broad interdisciplinary audience.
Comprehensive Biophysics
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Author :
language : en
Publisher: Academic Press
Release Date : 2012-04-12
Comprehensive Biophysics written by and has been published by Academic Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-04-12 with Science categories.
Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource