Gene Delivery To Mammalian Cells
DOWNLOAD
Download Gene Delivery To Mammalian Cells PDF/ePub or read online books in Mobi eBooks. Click Download or Read Online button to get Gene Delivery To Mammalian Cells book now. This website allows unlimited access to, at the time of writing, more than 1.5 million titles, including hundreds of thousands of titles in various foreign languages. If the content not found or just blank you must refresh this page
Gene Delivery To Mammalian Cells
DOWNLOAD
Author : William C. Heiser
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-02-03
Gene Delivery To Mammalian Cells written by William C. Heiser and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-02-03 with Science categories.
Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Gene Transfer And Expression In Mammalian Cells
DOWNLOAD
Author : S.C. Makrides
language : en
Publisher: Gulf Professional Publishing
Release Date : 2003-10-24
Gene Transfer And Expression In Mammalian Cells written by S.C. Makrides and has been published by Gulf Professional Publishing this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-24 with Science categories.
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.
Gene Delivery To Mammalian Cells
DOWNLOAD
Author : William C. Heiser
language : en
Publisher: Humana Press
Release Date : 2003-10-28
Gene Delivery To Mammalian Cells written by William C. Heiser and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-28 with Science categories.
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.
Gene Delivery To Mammalian Cells
DOWNLOAD
Author : William C. Heiser
language : en
Publisher: Humana Press
Release Date : 2010-11-10
Gene Delivery To Mammalian Cells written by William C. Heiser and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2010-11-10 with Science categories.
Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Gene Delivery To Mammalian Cells
DOWNLOAD
Author : William C. Heiser
language : en
Publisher: Humana Press
Release Date : 2003-10-28
Gene Delivery To Mammalian Cells written by William C. Heiser and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-28 with Science categories.
The efficiency of delivering DNA into mammalian cells has increased t- mendously since DEAE dextran was first shown to be capable of enhancing transfer of RNA into mammalian cells in culture. Not only have other chemical methods been developed and refined, but also very efficient physical and viral delivery methods have been established. The technique of introducing DNA into cells has developed from transfecting tissue culture cells to delivering DNA to specific cell types and organs in vivo. Moreover, two important areas of biology—assessment of gene function and gene therapy—require succe- ful DNA delivery to cells, driving the practical need to increase the efficiency and efficacy of gene transfer both in vitro and in vivo. TM These two volumes of the Methods in Molecular Biology series, Gene Del- ery to Mammalian Cells, are designed as a compendium of those techniques that have proven most useful in the expanding field of gene transfer in mammalian cells. It is intended that these volumes will provide a thorough background on chemical, physical, and viral methods of gene delivery, a synopsis of the myriad techniques currently available to introduce genes into mammalian cells, as well as a practical guide on how to accomplish this. It is my expectation that it will be useful to the novice in the field as well as to the scientist with expertise in gene delivery.
Gene Delivery To Mammalian Cells
DOWNLOAD
Author : William C. Heiser
language : en
Publisher: Humana
Release Date : 2003-10-28
Gene Delivery To Mammalian Cells written by William C. Heiser and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2003-10-28 with Science categories.
Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Non Viral Gene Therapy
DOWNLOAD
Author : Kazunari Taira
language : en
Publisher: Springer Science & Business Media
Release Date : 2006-03-20
Non Viral Gene Therapy written by Kazunari Taira and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2006-03-20 with Science categories.
Presents information on non-viral gene-delivery techniques, covering a spectrum of disciplines that include chemistry, molecular biology, cell biology, and pharmacokinetics. This work is useful to researchers and engineers in genetic engineering, molecular medicine, biochemical engineering, and biotechnology.
Gene Transfer To Animal Cells
DOWNLOAD
Author : Richard Twyman
language : en
Publisher: Taylor & Francis
Release Date : 2004-12-01
Gene Transfer To Animal Cells written by Richard Twyman and has been published by Taylor & Francis this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-12-01 with Science categories.
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Gene Therapeutics
DOWNLOAD
Author : Jon A. Wolff
language : en
Publisher: Springer Science & Business Media
Release Date : 2012-12-06
Gene Therapeutics written by Jon A. Wolff and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2012-12-06 with Medical categories.
During the first half century of genetics, coinciding with the first half of this cen tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex tremely efficient in transferring their contents to the chromosomes of cultured cells.
Artificial Self Assembling Systems For Gene Delivery
DOWNLOAD
Author : Philip L. Felgner
language : en
Publisher:
Release Date : 1996
Artificial Self Assembling Systems For Gene Delivery written by Philip L. Felgner and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996 with Language Arts & Disciplines categories.
Discusses recent advances in artificial self-assembling systems, including retrotransposon vectors, hairpin ribozymes, triple-helix-forming oligonucleotides, liposome-mediated transfection, and photonic nanostructures. Addresses developing efficient synthetic vectors such as modular self-assembling systems mimicking important features of viral vectors. Presents new developments in synthetic self-assembling gene delivery systems, including innovations in nonviral systems, targeting nucleic acids, ligand-polylysine mediated transfer, dendrimer-mediated transfection, cationic liposomes, and polylysine DNA complexes.