Gene Expression In Recombinant Microorganisms
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Recombinant Protein Expression In Microbial Systems
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Author : Eduardo A. Ceccarelli
language : en
Publisher: Frontiers E-books
Release Date : 2014-10-02
Recombinant Protein Expression In Microbial Systems written by Eduardo A. Ceccarelli and has been published by Frontiers E-books this book supported file pdf, txt, epub, kindle and other format this book has been release on 2014-10-02 with Biotechnology categories.
With the advent of recombinant DNA technology, expressing heterologous proteins in microorganisms rapidly became the method of choice for their production at laboratory and industrial scale. Bacteria, yeasts and other hosts can be grown to high biomass levels efficiently and inexpensively. Obtaining high yields of recombinant proteins from this material was only feasible thanks to constant research on microbial genetics and physiology that led to novel strains, plasmids and cultivation strategies. Despite the spectacular expansion of the field, there is still much room for progress. Improving the levels of expression and the solubility of a recombinant protein can be quite challenging. Accumulation of the product in the cell can lead to stress responses which affect cell growth. Buildup of insoluble and biologically inactive aggregates (inclusion bodies) lowers the yield of production. This is particularly true for obtaining membrane proteins or high-molecular weight and multi-domain proteins. Also, obtaining eukaryotic proteins in a prokaryotic background (for example, plant or animal proteins in bacteria) results in a product that lack post-translational modifications, often required for functionality. Changing to a eukaryotic host (yeasts or filamentous fungi) may not be a proper solution since the pattern of sugar modifications is different than in higher eukaryotes. Still, many advances in the last couple of decades have provided to researchers a wide variety of strategies to maximize the production of their recombinant protein of choice. Everything starts with the careful selection of the host. Be it bacteria or yeast, a broad list of strains is available for overcoming codon use bias, incorrect disulfide bond formation, protein toxicity and lack of post-translational modifications. Also, a huge catalog of plasmids allows choosing for different fusion partners for improving solubility, protein secretion, chaperone co-expression, antibiotic resistance and promoter strength. Next, controlling culture conditions like temperature, inducer and media composition can bolster recombinant protein production. With this Research Topic, we aim to provide an encyclopedic account of the existing approaches to the expression of recombinant proteins in microorganisms, highlight recent discoveries and analyze the future prospects of this exciting and ever-growing field.
Gene Expression In Recombinant Microorganisms
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Author : Alan Smith
language : en
Publisher: CRC Press
Release Date : 1994-11-23
Gene Expression In Recombinant Microorganisms written by Alan Smith and has been published by CRC Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1994-11-23 with Science categories.
Describing the scientific and commercial applications of microbial recombinant DNA technology, this outstanding, single-source reference offers state-of-the-art reviews of gene expression in the most important classes of recombinant microorganisms-providing numerous examples of the expression of homologous genes or heterologous gene products. Presents a unique collection of safety and regulatory considerations from around the world and addresses specific measures to be taken for large-scale industrial operations!
Gene Expression Systems
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Author : Joseph M. Fernandez
language : en
Publisher: Elsevier
Release Date : 1998-12-21
Gene Expression Systems written by Joseph M. Fernandez and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-12-21 with Science categories.
Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. - Provides detailed information on expression systems - Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs - Includes timelines and examples - Compares pros and cons of each method
Molecular Biology Of The Cell
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Author :
language : en
Publisher:
Release Date : 2004
Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.
Recombinant Protein Production In Yeast
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Author : Brigitte Gasser
language : en
Publisher: Humana Press
Release Date : 2019-02-09
Recombinant Protein Production In Yeast written by Brigitte Gasser and has been published by Humana Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-02-09 with Science categories.
This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Basic And Applied Aspects Of Biotechnology
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Author : Varsha Gupta
language : en
Publisher: Springer
Release Date : 2016-10-22
Basic And Applied Aspects Of Biotechnology written by Varsha Gupta and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-10-22 with Technology & Engineering categories.
This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.
Safety Of Genetically Engineered Foods
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 2004-08-08
Safety Of Genetically Engineered Foods written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004-08-08 with Science categories.
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
New Directions For Biosciences Research In Agriculture
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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 1985-01-01
New Directions For Biosciences Research In Agriculture written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-01-01 with Technology & Engineering categories.
Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.
Microbial Biotechnology Providing Bio Based Components For The Food Industry
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Author : Laurent Dufossé
language : en
Publisher: Frontiers Media SA
Release Date : 2020-01-17
Microbial Biotechnology Providing Bio Based Components For The Food Industry written by Laurent Dufossé and has been published by Frontiers Media SA this book supported file pdf, txt, epub, kindle and other format this book has been release on 2020-01-17 with categories.