Gene Expression Systems

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Gene Expression Systems

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Author : Joseph M. Fernandez
language : en
Publisher: Elsevier
Release Date : 1998-12-21

Gene Expression Systems written by Joseph M. Fernandez and has been published by Elsevier this book supported file pdf, txt, epub, kindle and other format this book has been release on 1998-12-21 with Science categories.

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression. - Provides detailed information on expression systems - Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs - Includes timelines and examples - Compares pros and cons of each method

Cell Free Gene Expression

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Author : Ashty S. Karim
language : en
Publisher: Humana
Release Date : 2022-01-06

Cell Free Gene Expression written by Ashty S. Karim and has been published by Humana this book supported file pdf, txt, epub, kindle and other format this book has been release on 2022-01-06 with Science categories.

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell‐Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

Expression Systems

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Author : Michael Dyson
language : en
Publisher: Scion Publishing Ltd
Release Date : 2007-09-15

Expression Systems written by Michael Dyson and has been published by Scion Publishing Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2007-09-15 with Science categories.

1. Expression strategy (Michael Dyson) 2. Protein expression in Escherichia coli (Rosalind Kim) 3. Expression engineering of synthetic antibodies using ribosome display (Matthew DeLisa and Lydia M. Contreras Martinez) 4. Refolding proteins from inclusion bodies (Renaud Vincentelli) 5. Selection of protein variants with improved expression using GFP-derived folding and solubility reporters (Geoffrey Waldo and Stéphanie Cabantous) 6. Protein expression in the wheat germ cell-free system (Yaeta Endo and Tatsuya Sawasaki) 7. Saccharomyces cerevisiae ; A microbial eukaryotic expression system (Christine Lang) 8. Expression of proteins in Pichia pastoris (Geoff and Joan Lin-Cereghino and Wilson Leung) 9. Improved baculovirus expression vectors (Linda King, Richard Hitchman and Robert Possee) 10. Transient transfection of insect cells for rapid expression screening and protein production (Robert Novy et al.) 11. Generation of stable CHO cell lines for protein expression (Zhijian Lu et al.) 12. Transient expression in HEK293-EBNA1 cells (Yves Durocher, Roseanne Tom and Louis Bisson) 13. Nisin- and subtilin-controlled gene expression systems for Gram-positive bacteria (Oscar Kuipers and Jan Kok) 14. Protein expression using lentiviral vectors (Bernard Massie, Renald Gilbert and Sophie Broussau) 15. Expression in mammalian cells using BacMam viruses (Yu-Chen Hu and Hsiao-Ping Lee) List of suppliers;Index

Regulation Of Gene Expression

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Author : Gary H. Perdew
language : en
Publisher: Springer Science & Business Media
Release Date : 2008-08-17

Regulation Of Gene Expression written by Gary H. Perdew and has been published by Springer Science & Business Media this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008-08-17 with Science categories.

The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Gene Structure And Expression

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Author : John D. Hawkins
language : en
Publisher: Cambridge University Press
Release Date : 1996-09-12

Gene Structure And Expression written by John D. Hawkins and has been published by Cambridge University Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1996-09-12 with Science categories.

This third edition of a successful textbook is a concise description of the structure and function of genes.

Molecular Biology Of The Cell

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Author :
language : en
Publisher:
Release Date : 2004

Molecular Biology Of The Cell written by and has been published by this book supported file pdf, txt, epub, kindle and other format this book has been release on 2004 with Cells categories.

Gene Expression Systems In Fungi Advancements And Applications

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Author : Monika Schmoll
language : en
Publisher: Springer
Release Date : 2016-04-04

Gene Expression Systems In Fungi Advancements And Applications written by Monika Schmoll and has been published by Springer this book supported file pdf, txt, epub, kindle and other format this book has been release on 2016-04-04 with Science categories.

Biotechnology has emerged as one of the key environmentally safe technologies for the future which enables use of biomass to develop novel smart materials and to replace oil derived products. Fungi are the most efficient producers of the enzymes needed for this purpose and in addition they produce a plethora of secondary metabolites, among which novel antibiotics can be found. Industrial application and exploitation of the metabolic capacities of fungi requires highly productive and robust gene expression systems, which can be achieved by selection of appropriate species and strain improvement. In this book we aim to summarize homologous and heterologous gene expression systems of fungi for production of enzymes and secondary metabolites. A broad overview on requirements, challenges and successful applications shall serve as a basis for further development of fungi as biotechnological workhorses in research and industry.

New Directions For Biosciences Research In Agriculture

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Author : National Research Council
language : en
Publisher: National Academies Press
Release Date : 1985-01-01

New Directions For Biosciences Research In Agriculture written by National Research Council and has been published by National Academies Press this book supported file pdf, txt, epub, kindle and other format this book has been release on 1985-01-01 with Technology & Engineering categories.

Authored by an integrated committee of plant and animal scientists, this review of newer molecular genetic techniques and traditional research methods is presented as a compilation of high-reward opportunities for agricultural research. Directed to the Agricultural Research Service and the agricultural research community at large, the volume discusses biosciences research in genetic engineering, animal science, plant science, and plant diseases and insect pests. An optimal climate for productive research is discussed.

Recent Advances In Plant Biotechnology And Its Applications

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Author : Ashwani Kumar
language : en
Publisher: I. K. International Pvt Ltd
Release Date : 2008

Recent Advances In Plant Biotechnology And Its Applications written by Ashwani Kumar and has been published by I. K. International Pvt Ltd this book supported file pdf, txt, epub, kindle and other format this book has been release on 2008 with Science categories.

This book is divided into five sections. The first section deals with the methodology and bioresource generation, techniques related to genetic engineering, and gene transfer to the nuclear genome and chloroplast genome. The new techniques of genome profiling and gene silencing are also presented. The second section of the book covers the classical aspect of plant biotechnology viz. tissue culture and micropropagation. Use of genetic engineering via Agrobacterium and direct transfer of DNA through particle bombardment to develop transformed plants in Artemisia, castor and orchids, and production of recombinant proteins in plant cells have been dealt with in the third section. The fourth section addresses the abiotic and biotic stress tolerance in plants. The basic biology of some of the stress responses, and designing plants for stress tolerance is discussed in this section. The fifth section examines medicinal plants and alkaloid production.

Introduction To Basics Of Pharmacology And Toxicology

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Author : Gerard Marshall Raj
language : en
Publisher: Springer Nature
Release Date : 2019-11-16

Introduction To Basics Of Pharmacology And Toxicology written by Gerard Marshall Raj and has been published by Springer Nature this book supported file pdf, txt, epub, kindle and other format this book has been release on 2019-11-16 with Medical categories.

This book illustrates, in a comprehensive manner, the most crucial principles involved in pharmacology and allied sciences. The title begins by discussing the historical aspects of drug discovery, with up to date knowledge on Nobel Laureates in pharmacology and their significant discoveries. It then examines the general pharmacological principles - pharmacokinetics and pharmacodynamics, with in-depth information on drug transporters and interactions. In the remaining chapters, the book covers a definitive collection of topics containing essential information on the basic principles of pharmacology and how they are employed for the treatment of diseases. Readers will learn about special topics in pharmacology that are hard to find elsewhere, including issues related to environmental toxicology and the latest information on drug poisoning and treatment, analytical toxicology, toxicovigilance, and the use of molecular biology techniques in pharmacology. The book offers a valuable resource for researchers in the fields of pharmacology and toxicology, as well as students pursuing a degree in or with an interest in pharmacology.